Abstract: A water-soluble polysaccharide gel having good gelling property and good antimicrobial activity was extracted from fruit-rind of durian (Durio zibethinus Murr.) comprises steps of boiling dried fruit rind with 25-30 volumes by weight of water, filtering, repeating boiling residue 1-2 times with 15 volume by weight of water. Filtrates were pooled and concentrated by rotary evaporating at 70° C. under reduced pressure and precipitating using acidified aqueous ethanol, collecting precipitate, spray-drying or drying and pulverizing. Pharmaceutical preparations were prepared using the polysaccharide gel such as anti-acne preparations, alcohol-free hand sanitizer preparation and wound antibacterial gel preparation.