Patents by Inventor Vineet Rajgarhia
Vineet Rajgarhia has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11634735Abstract: The present invention provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, upregulated or downregulated.Type: GrantFiled: July 1, 2020Date of Patent: April 25, 2023Assignee: Lallemand Hungary Liquidity Management LLCInventors: John E. McBride, Vineet Rajgarhia, Arthur J. Shaw, IV, Shital A. Tripathi, Elena Brevnova, Nicky Caiazza, Johannes Pieter Van Dijken, Allan C. Froehlich, William Ryan Sillers, James H. Flatt
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Publication number: 20210388398Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluyveromyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.Type: ApplicationFiled: December 22, 2020Publication date: December 16, 2021Inventors: John McBride, Elena Brevnova, Mark Mellon, Allan Froehlich, Kristen Deleault, Vineet Rajgarhia, Riaan Den Haan, Merja Penttila, Marja Ilmen, Matti Siika-Aho, Jaana Uusitalo, Emily A. Stonehouse, Alan Gilbert, Haowen Xu, Deidre Willies, John Bardsley, Anu Koivula, Sanni Voutilainen
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Publication number: 20200325500Abstract: The present invention provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, upregulated or downregulated.Type: ApplicationFiled: July 1, 2020Publication date: October 15, 2020Inventors: John E. McBride, Vineet Rajgarhia, Arthur J. Shaw, IV, Shital A. Tripathi, Elena Brevnova, Nicky Caiazza, Johannes Pieter Van Dijken, Allan C. Froehlich, William Ryan Sillers, James H. Flatt
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Patent number: 10428322Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: GrantFiled: January 10, 2019Date of Patent: October 1, 2019Assignee: Lallemand Hungary Liquidity Management LLCInventors: Elena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall
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Publication number: 20190127714Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: ApplicationFiled: January 10, 2019Publication date: May 2, 2019Inventors: Elena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall
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Patent number: 10214733Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: GrantFiled: November 28, 2017Date of Patent: February 26, 2019Assignee: Lallemand Hungary Liquidity Management LLCInventors: Elena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall
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Publication number: 20180258449Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluyveromyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.Type: ApplicationFiled: May 21, 2018Publication date: September 13, 2018Inventors: John McBride, Elena Brevnova, Mark Mellon, Allan Froehlich, Kristen Deleault, Vineet Rajgarhia, Riaan Den Haan, Merja Penttila, Marja Ilmen, Matti Siika-Aho, Jaana Uusitalo, Emily A. Stonehouse, Alan Gilbert, Haowen Xu, Deidre Willies, John Bardsley, Anu Koivula, Sanni Voutilainen
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Publication number: 20180208951Abstract: The present in provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, upregulated or downregulated.Type: ApplicationFiled: March 21, 2018Publication date: July 26, 2018Inventors: John E. McBride, Vineet Rajgarhia, Arthur J. Shaw, IV, Shital A. Tripathi, Elena Brevnova, Nicky Caiazza, Johannes Pieter Van Dijken, Allan C. Froehlich, William Ryan Sillers, James H. Flatt
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Publication number: 20180155700Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: ApplicationFiled: November 28, 2017Publication date: June 7, 2018Inventors: Elena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall
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Patent number: 9988652Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluveryomyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.Type: GrantFiled: July 1, 2015Date of Patent: June 5, 2018Assignees: Lallemand Hungary Liquidity Management LLC, Stellenbosch UniversityInventors: John McBride, Elena Brevnova, Mark Mellon, Allan Froehlich, Kristen Deleault, Vineet Rajgarhia, Riaan Den Haan, Merja Penttila, Marja Ilmen, Matti Siika-Aho, Jaana Uusitalo, Emily A. Stonehouse, Alan Gilbert, Haowen Xu, Deidre Willes, John Bardsley, Anu Koivula, Sanni Voutilainen
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Patent number: 9957530Abstract: The present invention provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, upregulated or downregulated.Type: GrantFiled: August 20, 2010Date of Patent: May 1, 2018Assignee: Lallemand Hungary Liquidity Management LLCInventors: John E. McBride, Vineet Rajgarhia, Arthur J. Shaw, IV, Shital A. Tripathi, Elena Brevnova, Nicky Caiazza, Johannes Pieter Van Dijken, Allan C. Froehlich, William Ryan Sillers, James H. Flatt
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Patent number: 9856465Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: GrantFiled: March 8, 2016Date of Patent: January 2, 2018Assignee: Lallemand Hungary Liquidity Management LLCInventors: Elena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall
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Patent number: 9758799Abstract: Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.Type: GrantFiled: July 5, 2016Date of Patent: September 12, 2017Assignee: Cargill IncorporatedInventors: Vineet Rajgarhia, Kari Koivuranta, Merja Penttila, Marja Ilmen, Pirkko Suominen, Aristos Aristidou, Christopher Kenneth Miller, Stacey Olson, Laura Ruohonen
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Publication number: 20160340697Abstract: Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.Type: ApplicationFiled: July 5, 2016Publication date: November 24, 2016Inventors: Vineet Rajgarhia, Kari Koivuranta, Merja Penttila, Marja Ilmen, Pirkko Suominen, Aristos Aristidou, Christopher Kenneth Miller, Stacey Olson, Laura Ruohonen
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Patent number: 9410158Abstract: Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.Type: GrantFiled: September 24, 2013Date of Patent: August 9, 2016Assignee: Cargill IncorporatedInventors: Vineet Rajgarhia, Kari Koivuranta, Merja Penttila, Marja Ilmen, Pirkko Suominen, Aristos Aristidou, Christopher Kenneth Miller, Stacey Olson, Laura Ruohonen
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Publication number: 20160177283Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: ApplicationFiled: March 8, 2016Publication date: June 23, 2016Inventors: Elena E. BREVNOVA, Vineet RAJGARHIA, Mark MELLON, Anne WARNER, John MCBRIDE, Chhayal GANDHI, Erin WISWALL
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Patent number: 9315833Abstract: The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction.Type: GrantFiled: February 18, 2010Date of Patent: April 19, 2016Assignees: Lallemand Hungary Liquidity Management LLC, Stellenbosch UniversityInventors: John McBride, Mark Mellon, Vineet Rajgarhia, Elena E. Brevnova, Erin Wiswall, David A. Hogsett, Danie LaGrange, Shaunita Rose, Emile Van Zyl
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Publication number: 20160010117Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluveryomyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.Type: ApplicationFiled: July 1, 2015Publication date: January 14, 2016Inventors: John McBride, Elena Brevnova, Mark Mellon, Allan Froehlich, Kristen Deleault, Vineet Rajgarhia, Riaan Den Haan, Merja Penttila, Marja Ilmen, Matti Siika-Aho, Jaana Uusitalo, Emily A. Stonehouse, Alan Gilbert, Haowen Xu, Deidre Willes, John Bardsley, Anu Koivula, Sanni Voutilainen
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Patent number: 9102955Abstract: Host cells, comprising Kluveryomyces expressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes.Type: GrantFiled: November 23, 2009Date of Patent: August 11, 2015Assignee: Lallemand Hungary Liquidity Management LLCInventors: John McBride, Elena Brevnova, Mark Mellon, Allan Froehlich, Kristen Deleault, Vineet Rajgarhia, Riaan Den Haan, Merja Penttila, Marja Ilmen, Matti Siika-Aho, Jaana Uusitalo, Emily A. Stonehouse, Alan Gilbert, Haowen Xu, Deidre Willes, John Bardsley, Anu Koivula, Sanni Voutilainen
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Publication number: 20150024438Abstract: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: ApplicationFiled: February 24, 2014Publication date: January 22, 2015Applicant: Mascoma CorporationInventors: Elena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall