Abstract: The present invention disclosed a metal nanoparticles/single-walled carbon nanotube (MNP/SWCNT) hybrid based chemiresistive biosensor for the quantitative detection of human cardiac biomarkers troponin I (cTnI) and myoglobin (Mb). The highly specific cardiac-antibody, anti-cTnI (Ab-cTnI) or anti-Mb (Ab-Mb), was covalently immobilized to site-specific carboxyl groups on MNP anchored over SWCNT device. The biosensor device was characterized by the source-drain current-voltage measurements. The device performance was investigated with a change in conductance in SWCNT channel upon exposure to cTnI in human serum. MNP provided large surface area for high protein loading and improved electrical signal by inducing charge density in SWCNT, resulting in low level detection of cTnI and Mb with high sensitivity.