Abstract: The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.

Abstract: The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.

Abstract: A method provides flow to multiple channels in an endoscope during an endoscope decontamination procedure. A member is inserted into a chamber in a body of an endoscope through an opening into the chamber, the chamber being intersected by a first lumen and a second lumen. The member separates fluidly the first lumen from the second lumen. Fluid is flowed through a first channel through the member into the first lumen and through a second channel through the member into the second lumen. Additional lumens can be so treated at the same time. Fluids can include detergents and other cleaning fluids, disinfectants and sterilants.

Abstract: A method provides flow to multiple channels in an endoscope during an endoscope decontamination procedure. A member is inserted into a chamber in a body of an endoscope through an opening into the chamber, the chamber being intersected by a first lumen and a second lumen. The member separates fluidly the first lumen from the second lumen. Fluid is flowed through a first channel through the member into the first lumen and through a second channel through the member into the second lumen. Additional lumens can be so treated at the same time. Fluids can include detergents and other cleaning fluids, disinfectants and sterilants.

Abstract: An endoscope processor, according to the present invention, provides for cleaning and sterilizing an endoscope having a body, and a first flexible tube attached to the body. It includes an operational housing and an enclosure which is attachable to and detachable from the operational housing, the enclosure being sealed from ingress of potentially contaminating microorganisms when detached from the operational housing. The enclosure is shaped, sized and oriented to receive the endoscope in an orientation with the first flexible tube depending vertically downwardly from the endoscope body. The enclosure includes a liquid inlet and a liquid outlet.

Abstract: The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.

Abstract: A device for performing polymerase chain reactions using cooling members not used in conventional thermal cyclers. A device for performing polymerase chain reactions may include a sample holder configured to receive a nucleic acid sample, a heating system configured to raise the temperature of the sample, a cooling system configured to lower the temperature of the sample, and a controller configured to operably control the heating system and the cooling system to cycle the device through a desired time-temperature profile. The cooling system may comprise at least one cooling member selected from a synthetic jet ejector array, a vibration-induced droplet atomization system, a vibrating diaphragm system, a piezo fan, a Cold Gun, a microchannel cooling loop, and a Cool Chip.

Abstract: A device for performing biological analysis may include at least one reaction chamber configured to receive at least one sample for biological analysis and a thermal system configured to modulate a temperature of the at least one reaction chamber to cycle a temperature of the at least one biological sample. The thermal system may include a cooling system configured to cool the at least one reaction chamber. The cooling system may include a cooling fluid source positioned distally from the at least one reaction chamber, the cooling fluid source being in flow communication with at least one conduit configured to flow cooling fluid from the cooling fluid source to at least one location in thermal communication with the at least one reaction chamber.

Abstract: The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.

Abstract: An endoscope enclosure for sterilizing an endoscope having a body, and a first flexible tube attached to the body. The endoscope enclosure has an interior space for receiving the endoscope in an orientation with the first flexible tube depending vertically downwardly from the endoscope body. It includes both an inlet fluid connector and an outlet fluid connector, each fluidly communicating with the interior space and which has an closure for isolating the interior space from an exterior environment therethrough. The interior space is sealed from the exterior environment so that an endoscope can be sterilized by passing fluid through the interior space via the inlet and outlet fluid connectors and stored therein in an orientation with the first flexible tube depending downwardly, and in sterile form being protected from ingress of potentially contaminating microorganisms.

Abstract: An endoscope processor, according to the present invention, provides for cleaning and sterilizing an endoscope having a body, and a first flexible tube attached to the body. It includes an operational housing and an enclosure which is attachable to and detachable from the operational housing, the enclosure being sealed from ingress of potentially contaminating microorganisms when detached from the operational housing. The enclosure is shaped, sized and oriented to receive the endoscope in an orientation with the first flexible tube depending vertically downwardly from the endoscope body. The enclosure includes a liquid inlet and a liquid outlet.

Abstract: A method for stabilizing the temperature of an LED is provided. A method is provided that includes providing a system comprising an LED, a reaction region, and a sample in the reaction region; generating excitation beams with the LED; directing excitation beams to the sample; detecting an optical property of the sample to obtain detection data; measuring the operating temperature of the light emitting diode; and adjusting the detection data of an excitation beam characteristic shift related to the operating temperature, when the LED is operated at the operating temperature to generate the excitation beams.

Abstract: A method provides flow to multiple channels in an endoscope during an endoscope decontamination procedure. A member is inserted into a chamber in a body of an endoscope through an opening into the chamber, the chamber being intersected by a first lumen and a second lumen. The member separates fluidly the first lumen from the second lumen. Fluid is flowed through a first channel through the member into the first lumen and through a second channel through the member into the second lumen. Additional lumens can be so treated at the same time. Fluids can include detergents and other cleaning fluids, disinfectants and sterilants.

Abstract: The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.

Abstract: The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.

Abstract: A soil standard for monitoring a cleaning process for a medical instrument, employs a soil which contains moisture so as to more accurately mimic soil which may be present upon an instrument to be cleaned in the cleaning process.

Abstract: The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.

Abstract: A system is provided that includes a light-emitting diode (LED); a temperature sensor in thermal contact with the LED and capable of measuring an operating temperature and generating an operating temperature signal; and a temperature regulating system capable of receiving the operating temperature signal and regulating the operating temperature based on the operating temperature signal. A method for stabilizing the temperature of an LED is provided. A method is provided that includes providing a system comprising an LED, a reaction region, and a sample in the reaction region; generating excitation beams with the LED; directing excitation beams to the sample; detecting an optical property of the sample to obtain detection data; measuring the operating temperature of the light emitting diode; and adjusting the detection data of an excitation beam characteristic shift related to the operating temperature, when the LED is operated at the operating temperature to generate the excitation beams.

Abstract: The invention provides for dynamic channel/capacity assignment within an 802.11 wireless data communications network (10) consisting of several access points (20). The access points are linked to each by inter access point channels 11. Each access point 20 has an associated local coverage area 13 which services roaming terminal devices 14 or local 802.11 stations. The invention allows for increased throughput between the access points or between an access point and local stations within the local coverage area of the access point (20) by allowing additional channel capacity to be dynamically re-assigned. The invention provides a degree of quality of service for multimedia traffic transfer in wireless networks such as the 802.11 based extended service set.

Abstract: A single rotating polygon mirror with v-shaped facets having upper and lower reflective facet surfaces reflects and separates dual beams to two photoreceptors in a ROS. Each facet surface will have a different tilt angle. The two independently modulated beams will share common optical elements between the light sources and the mirror and may share a common f-theta scan lens. Two sets of two beams can be incident upon the facets on opposite sides of the rotating polygon mirror. The polygon mirror facet can also have three or four reflective facet surfaces to reflect and separate three or four independently modulated beams to three or four different photoreceptors.