Abstract: A method of producing recombinant plasmid DNA using substantially solid growth medium and disposable vessels in place of conventional liquid fermentation processes. The method includes inoculating a host organism containing the recombinant plasmid DNA onto the substantially solid growth medium in a disposable vessel; allowing the host organism to grow on the growth medium under conditions conducive to such growth; removing the host organism from the growth medium and lysing the host organism to access the recombinant plasmid DNA; and purifying the recombinant plasmid DNA.

Abstract: A method of producing recombinant proteins, or polypeptides, in bacteria or other host cells grown on a substantially solid, i.e., solid or semi-solid, nutrient growth medium harboring DNA sequences of interest for encoding the recombinant proteins under the control of an inducer-regulated promoter or constitutive promoter. The bacteria are harvested from the solid or semi-solid nutrient medium and the desired recombinant protein is recovered by disrupting the surface of the bacteria.

Abstract: A method of producing recombinant proteins, or polypeptides, in bacteria or other host cells grown on a substantially solid, i.e., solid or semi-solid, nutrient growth medium harboring DNA sequences of interest for encoding the recombinant proteins under the control of an inducer-regulated promoter or constitutive promoter. The bacteria are harvested from the solid or semi-solid nutrient medium and the desired recombinant protein is recovered by disrupting the surface of the bacteria.