Abstract: Compositions and methods for targeting polypeptides to plant vacuoles are provided. Nucleic acid molecules having nucleotide sequences encoding a vacuole-targeting peptide, variants, or fragments thereof are provided. The sequences also can be used for targeting defensin proteins or other polypeptides to vacuoles in plants. Transformed plants, plant cells, tissues, and seed also are provided.

Abstract: The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a tissue-preferred maize promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the tissue-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

Abstract: The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a tissue-preferred maize promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the tissue-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

Abstract: The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a tissue-preferred maize promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the tissue-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

Abstract: Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided.

Abstract: The invention relates to compositions including amino acid sequences isolated from fungal fermentation broths and methods for protecting a plant from a pathogen, particularly a fungal pathogen.

Abstract: The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a tissue-preferred maize promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the tissue-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

Abstract: Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided.

Abstract: The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a tissue-preferred maize promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the tissue-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

Abstract: Methods and compositions for modulating development and defense response are provided. Nucleotide sequences encoding a maize proteinase inhibitor-like protein is provided. Nucleotide sequences comprising the proteinase inhibitor-like promoter are also provided. Methods of using the promoter to express nucleic acid sequences of interest are provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and the plant defense response. Plants, plant cells, tissues, and seed transformed with the proteinase inhibitor-like gene or its promoter are also provided.

Abstract: Methods and compositions for modulating development and defense response are provided. Nucleotide sequences encoding a maize proteinase inhibitor-like protein is provided. Nucleotide sequences comprising the proteinase inhibitor-like promoter are also provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and the plant defense response. Transformed plants, plant cells, tissues, and seed are also provided.

Abstract: The invention relates to the genetic manipulation of plants, particularly to the expression of genes involved in oxylipin metabolism in plants. Nucleotide sequences encoding homologues of Old Yellow Enzyme, and inducible promoters and proteins thereof, are provided. The sequences find use in modifying oxylipin metabolism in plants, increasing the resistance of plants to stress, regulating gene expression in plants, and in the production of oxylipins in plants.

Abstract: Histone deacetylases and nucleotide sequences encoding said histone deacetylases are provided. The sequences as well as corresponding antisense constructs are useful for modulating gene activity in plants. Additionally, the sequences are useful for enhancing disease resistance in transformed plants.

Abstract: The present invention provides compositions and methods for enhancing the disease resistance of plants. The compositions are novel nucleic acid molecules isolated from maize, which encode pathogenesis-related (PR1) proteins, and the PR1 proteins encoded thereby. The methods comprise introducing into a plant cell at least one nucleotide construct comprising a PR1 nucleotide sequence of the invention operably linked to a promoter that drives expression in a plant cell. The methods additionally involve regenerating a stably transformed plant from the transformed plant cell. Transformed plants and seeds having enhanced disease resistance are also provided.

Abstract: The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for inducible and constitutive promoters isolated from a family of maize genes encoding pathogenesis-related (PR-1) proteins. Methods for regulating expression of a heterologous nucleotide sequence in a plant in an inducible or constitutive manner are provided. The methods comprise transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the inducible or constitutive promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell. Transformed plants and seeds are also provided.

Abstract: The invention provides isolated WRKY nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering WRKY concentration and/or composition of plants. The present invention also relates to transcriptional regulatory regions of WRKY polynucleotides and their use to regulate heterologous gene expression. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.

Abstract: Histone deacetylases and nucleotide sequences encoding said histone deacetylases are provided. The sequences as well as corresponding antisense constructs are useful for modulating gene activity in plants. Additionally, the sequences are useful for enhancing disease resistance in transformed plants.

Abstract: The invention provides isolated PR1-C10 nucleic acids and their encoded polypeptides. The present invention provides methods and compositions relating to altering PR1-C10 concentration and/or composition of plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.

Abstract: The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for inducible and constitutive promoters isolated from a family of maize genes encoding pathogenesis-related (PR-1) proteins, nucleotide sequences for these maize PR-1 genes, and amino acid sequences for the PR-1 proteins encoded thereby. Methods for regulating expression of a heterologous nucleotide sequence in a plant in an inducible or constitutive manner are provided, more particularly regulating expression of a PR-1 gene to enhance disease resistance in a plant. The methods comprise transforming a plant cell to comprise a heterologous nucleotide sequence or PR-1 gene operably linked to one of the inducible or constitutive promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell. Transformed plants and seeds having enhanced disease resistance are also provided.

Abstract: Histone deacetylases and nucleotide sequences encoding said histone deacetylases are provided. The sequences as well as corresponding antisense constructs are useful for modulating gene activity in plants. Additionally, the sequences are useful for enhancing disease resistance in transformed plants.