Abstract: A method is disclosed for preparing a purified extraction residue fraction (antigen) of a microorganism isolated from the blood or urine of a warm-blooded animal or tumor carried by a warm-blooded animal, the microorganism having the capacity to synthesize the polypeptide hormone known as "chorionic gonadotropin" in its total form or in its alpha and beta subunits. The purified extraction residue fraction is an activator and modulator of immunological response and is capable of evoking pronounced prophylactic and therapeutic effects against a variety of tumors in laboratory animals and man. The immune response is stimulated by administering an effective immuno-stimulating amount of the residue fraction and/or co-administering the residue fraction together with an adjuvant such as the complete Freund's adjuvant.

Abstract: Method for the production of chorionic gonadotropin (CG) with properties similar to those of human CG (HCG) from a microorganism or mutant thereof isolated by natural or hybridization procedure from the body or body extract carrier of a malignant tumor carrier and having the capacity to synthesize the polypeptide hormone known as human chorionic gonadotropin in its total form or in its sub-units (.alpha. & .beta.) which comprises:(a) culturing the microorganism or mutant thereof in a culture media;(b) incubating the culture of the microorganism or mutant thereof, whereby the microorganism or mutant thereof in vivo produces a crude material containing chorionic gonadotropin and/or its sub-units (.alpha. & .beta.);(c) separating the crude material containing chorionic gonadotropin and/or its sub-units (.alpha. & .beta.) from the culture media and the microorganism or mutant thereof.

Abstract: A method is disclosed for preparing a purified extraction residue fraction (antigen) of a microorganism isolated from the blood or urine of a warm-blooded animal or tumor carried by a warm-blooded animal, the microorganism having the capacity to synthesize the polypeptide hormone known as "chorionic gonadotropin" in its total form or in its alpha and beta subunits. The purified extraction residue fraction is an activator and modulator of immunological response and is capable of evoking pronounced prophylactic and therapeutic effects against a variety of tumors in laboratory animals and man. The immune response is stimulated by administering an effective immunostimulating amount of the residue fraction.