Patents by Inventor Virginijus {hacek over (S)}ik{hacek over (s)}nys

Virginijus {hacek over (S)}ik{hacek over (s)}nys has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • RNA-directed DNA cleavage by the Cas9-crRNA complex
    Patent number: 11555187
    Abstract: Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule in vitro or in vivo using an RNA-guided DNA endonuclease comprising RNA sequences and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro and using a cassette containing a single repeat-spacer-repeat unit.
    Type: Grant
    Filed: December 7, 2017
    Date of Patent: January 17, 2023
    Assignee: Vilnius University
    Inventors: Virginijus {hacek over (S)}ik{hacek over (s)}nys, Giedrius Gasiunas, Tautvydas Karvelis, Arvydas Lubys, Lolita Zaliauskiene, Monika Gasiuniene, Anja Smith
  • Targeted RNA knockdown and knockout by type III-A Csm complexes
    Patent number: 11274317
    Abstract: Methods and compositions using a CRISPR-Cas Type IIIA resulting in RNA gene knockdown and knockout in an animal.
    Type: Grant
    Filed: August 31, 2017
    Date of Patent: March 15, 2022
    Assignee: VILNIUS UNIVERSITY
    Inventors: Thomas Fricke, Matthias Bochtler, Gintautas Tamulaitis, Miglé Kazlauskiene, Virginijus {hacek over (S)}ik{hacek over (s)}nys
  • RNA-directed DNA cleavage by the Cas9-crRNA complex
    Patent number: 10844378
    Abstract: Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule using an RNA-guided DNA endonuclease comprising at least one RNA sequence and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro or using a cassette containing a single repeat-spacer-repeat unit.
    Type: Grant
    Filed: October 1, 2018
    Date of Patent: November 24, 2020
    Assignee: Vilnius University
    Inventors: Virginijus {hacek over (S)}ik{hacek over (s)}nys, Giedrius Gasiūnas, Tautvydas Karvelis
  • RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX
    Publication number: 20190085329
    Abstract: Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule using an RNA-guided DNA endonuclease comprising at least one RNA sequence and at least one clan RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro or using a cassette containing a single repeat-spacer-repeat unit.
    Type: Application
    Filed: October 1, 2018
    Publication date: March 21, 2019
    Applicant: Vilnius University
    Inventors: Virginijus {hacek over (S)}IK{hacek over (S)}NYS, Giedrius Gasiunas, Tautvydas Karvelis
  • RNA-directed DNA cleavage by the Cas9-crRNA complex
    Patent number: 9637739
    Abstract: Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule using an RNA-guided DNA endonuclease comprising at least one RNA sequence and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro or using a cassette containing a single repeat-spacer-repeat unit.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: May 2, 2017
    Assignee: VILNIUS UNIVERSITY
    Inventors: Virginijus {hacek over (S)}ik{hacek over (s)}nys, Giedrius Gasiunas, Tautvydas Karvelis