Patents by Inventor Virginijus Siksnys
Virginijus Siksnys has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220356485Abstract: Compositions and methods are provided for rapid characterization of Cas endonuclease systems and the elements comprising such systems, including, but not limiting to, rapid characterization of PAM sequences, guide RNA elements and Cas endonucleases. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri MIc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.Type: ApplicationFiled: May 19, 2022Publication date: November 10, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, GIEDRIUS GASIUNAS, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K. YOUNG
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Patent number: 11371050Abstract: Compositions and methods are provided for rapid characterization of Cas endonuclease systems and the elements comprising such systems, including, but not limiting to, rapid characterization of PAM sequences, guide RNA elements and Cas endonucleases. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri Mlc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.Type: GrantFiled: May 12, 2016Date of Patent: June 28, 2022Inventors: Andrew Mark Cigan, Giedrius Gasiunas, Tautvydas Karvelis, Virginijus Siksnys, Joshua K Young
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Publication number: 20220073890Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.Type: ApplicationFiled: December 13, 2019Publication date: March 10, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ZHENGLIN HOU, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG
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Publication number: 20220010293Abstract: Compositions and methods are provided for novel Cas9 orthologs, including, but not limiting to, novel guide polynucleotide/Cas9 endonucleases complexes, single or dual guide RNAs, guide RNA elements, and Cas9 endonucleases. The present disclosure also describes methods for creating a double strand break in a target polynucleotide, methods for genome modification of a target sequence under various in vivo and in vitro conditions, in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell. Also provided are nucleic acid constructs and cells having a modified target site or altered polynucleotide of interest produced by the methods described herein.Type: ApplicationFiled: February 22, 2019Publication date: January 13, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ZHENGLIN HOU, JOSHUA K YOUNG, GIEDRIUS GASIUNAS, VIRGINIJUS SIKSNYS
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Publication number: 20210163908Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.Type: ApplicationFiled: January 21, 2021Publication date: June 3, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ZHENGLIN HOU, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG
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Publication number: 20210147909Abstract: Methods and compositions are provided for the identification, detection, characterization, and/or utilization of double strand breaks in a target polynucleotide; the identification, detection, characterization, and/or utilization of cutting sites for double-strand-break-inducing agents; and the identification, detection, characterization, and/or utilization of double-strand-break-inducing agents.Type: ApplicationFiled: May 10, 2019Publication date: May 20, 2021Applicants: PIONEER HI-BRED INTERNATIONAL, INC., VILNIUS UNIVERSITYInventors: STEPHANE DESCHAMPS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG, MINDAUGAS ZAREMBA
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Publication number: 20210139874Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.Type: ApplicationFiled: January 21, 2021Publication date: May 13, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ZHENGLIN HOU, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG
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Publication number: 20210130799Abstract: A method of synthetizing cyclic oligoadenylates using a novel catalytic activity of a protein possessing the Palm domain, such as the Cas10 protein, and using such compounds for activation of proteins possessing the CARF doman, such as the Csm6 protein.Type: ApplicationFiled: May 31, 2018Publication date: May 6, 2021Inventors: Virginijus SIKSNYS, Migle KAZLAUSKIENE, Georgij KOSTIUK, Gintautas TAMULAITIS
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Patent number: 10934536Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.Type: GrantFiled: December 13, 2019Date of Patent: March 2, 2021Assignee: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Zhenglin Hou, Tautvydas Karvelis, Virginijus Siksnys, Joshua K Young
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Publication number: 20200190494Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.Type: ApplicationFiled: December 13, 2019Publication date: June 18, 2020Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ZHENGLIN HOU, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG
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Publication number: 20190264232Abstract: Compositions and methods are provided for novel Cas9 orthologs, including, but not limiting to, novel guide polynucleotide/Cas9 endonucleases complexes, single or dual guide RNAs, guide RNA elements, and Cas9 endonucleases. The present disclosure also describes methods for creating a double strand break in a target polynucleotide, methods for genome modification of a target sequence under various in vivo and in vitro conditions, in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell. Also provided are nucleic acid constructs and cells having a modified target site or altered polynucleotide of interest produced by the methods described herein.Type: ApplicationFiled: February 22, 2019Publication date: August 29, 2019Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ZHENGLIN HOU, JOSHUA K. YOUNG, GIEDRIUS GASIUNAS, VIRGINIJUS SIKSNYS
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Patent number: 10385336Abstract: A Type III-A CRISPR-Cas (StCsm) complex of Streptococcus thermophilus comprising crRNA, Csm4, and Csm3 and use for cleavage of RNA bearing a nucleotide sequence complementary to the crRNA, in vitro or in vivo. Methods for site-specific cleavage/shredding of a target RNA molecule using an RNA-guided RNA endonuclease comprising a minimal complex of crRNA, Csm4, and Csm3, and methods of RNA knock-down and RNA knock-out are disclosed.Type: GrantFiled: March 3, 2017Date of Patent: August 20, 2019Assignee: VILNIUS UNIVERSITYInventors: Virginijus Siksnys, Migle Kazlauskiene, Gintautas Tamulaitis
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Publication number: 20190136248Abstract: Compositions and methods are provided for novel guide RNA/Cas endonuclease systems. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri MIc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.Type: ApplicationFiled: May 12, 2016Publication date: May 9, 2019Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, GIEDRIUS GASIUNAS, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG
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Publication number: 20180346895Abstract: Compositions and methods are provided for novel guide RNA/Cas endonuclease systems. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri Mlc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.Type: ApplicationFiled: July 25, 2018Publication date: December 6, 2018Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, GIEDRIUS GASIUNAS, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K. YOUNG
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Publication number: 20180258417Abstract: Compositions and methods are provided for rapid characterization of Cas endonuclease systems and the elements comprising such systems, including, but not limiting to, rapid characterization of PAM sequences, guide RNA elements and Cas endonucleases. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri MIc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.Type: ApplicationFiled: May 12, 2016Publication date: September 13, 2018Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, GIEDRIUS GASIUNAS, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG
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Publication number: 20180187195Abstract: Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule in vitro or in vivo using an RNA-guided DNA endonuclease comprising RNA sequences and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro and using a cassette containing a single repeat-spacer-repeat unit.Type: ApplicationFiled: December 7, 2017Publication date: July 5, 2018Applicant: VILNIUS UNIVERSITYInventors: Virginijus SIKSNYS, Giedrius GASIUNAS, Tautvydas KARVELIS, Arvydas LUBYS, Lolita ZALIAUSKIENE, Monika GLEMZAITE, Anja SMITH
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Publication number: 20180105835Abstract: Methods and compositions using a CRISPR-Cas Type IIIA resulting in RNA gene knockdown and knockout in an animal.Type: ApplicationFiled: August 31, 2017Publication date: April 19, 2018Inventors: Thomas FRICKE, Matthias BOCHTLER, Gintautas TAMULAITIS, Migle KAZLAUSKIENE, Virginijus SIKSNYS
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Publication number: 20170198286Abstract: A Type III-A CRISPR-Cas (StCsm) complex of Streptococcus thermophilus comprising crRNA, Csm4, and Csm3 and use for cleavage of RNA bearing a nucleotide sequence complementary to the crRNA, in vitro or in vivo. Methods for site-specific cleavage/shredding of a target RNA molecule using an RNA-guided RNA endonuclease comprising a minimal complex of crRNA, Csm4, and Csm3, and methods of RNA knock-down and RNA knock-out are disclosed.Type: ApplicationFiled: March 3, 2017Publication date: July 13, 2017Inventors: Virginijus Siksnys, Migle Kazlauskiene, Gintautas Tamulaitis
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Publication number: 20150291961Abstract: Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule using an RNA-guided DNA endonuclease comprising at least one RNA sequence and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one pint mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro or using a cassette containing a single repeat-spacer-repeat unit.Type: ApplicationFiled: June 18, 2015Publication date: October 15, 2015Inventors: Virginijus SIKSNYS, Giedrius GASIUNAS, Tautvydas KARVELIS
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Publication number: 20150240261Abstract: Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule using an RNA-guided DNA endonuclease comprising at least one RNA sequence and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro or using a cassette containing a single repeat-spacer-repeat unit.Type: ApplicationFiled: April 10, 2015Publication date: August 27, 2015Inventors: Virginijus SIKSNYS, Giedrius GASIÜNAS, Tautvydas KARVELIS