Patents by Inventor Virginijus Siksnys

Virginijus Siksnys has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220356485
    Abstract: Compositions and methods are provided for rapid characterization of Cas endonuclease systems and the elements comprising such systems, including, but not limiting to, rapid characterization of PAM sequences, guide RNA elements and Cas endonucleases. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri MIc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.
    Type: Application
    Filed: May 19, 2022
    Publication date: November 10, 2022
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ANDREW MARK CIGAN, GIEDRIUS GASIUNAS, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K. YOUNG
  • Patent number: 11371050
    Abstract: Compositions and methods are provided for rapid characterization of Cas endonuclease systems and the elements comprising such systems, including, but not limiting to, rapid characterization of PAM sequences, guide RNA elements and Cas endonucleases. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri Mlc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.
    Type: Grant
    Filed: May 12, 2016
    Date of Patent: June 28, 2022
    Inventors: Andrew Mark Cigan, Giedrius Gasiunas, Tautvydas Karvelis, Virginijus Siksnys, Joshua K Young
  • Publication number: 20220073890
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.
    Type: Application
    Filed: December 13, 2019
    Publication date: March 10, 2022
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ZHENGLIN HOU, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG
  • Publication number: 20220010293
    Abstract: Compositions and methods are provided for novel Cas9 orthologs, including, but not limiting to, novel guide polynucleotide/Cas9 endonucleases complexes, single or dual guide RNAs, guide RNA elements, and Cas9 endonucleases. The present disclosure also describes methods for creating a double strand break in a target polynucleotide, methods for genome modification of a target sequence under various in vivo and in vitro conditions, in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell. Also provided are nucleic acid constructs and cells having a modified target site or altered polynucleotide of interest produced by the methods described herein.
    Type: Application
    Filed: February 22, 2019
    Publication date: January 13, 2022
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ZHENGLIN HOU, JOSHUA K YOUNG, GIEDRIUS GASIUNAS, VIRGINIJUS SIKSNYS
  • Publication number: 20210163908
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.
    Type: Application
    Filed: January 21, 2021
    Publication date: June 3, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ZHENGLIN HOU, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG
  • Publication number: 20210147909
    Abstract: Methods and compositions are provided for the identification, detection, characterization, and/or utilization of double strand breaks in a target polynucleotide; the identification, detection, characterization, and/or utilization of cutting sites for double-strand-break-inducing agents; and the identification, detection, characterization, and/or utilization of double-strand-break-inducing agents.
    Type: Application
    Filed: May 10, 2019
    Publication date: May 20, 2021
    Applicants: PIONEER HI-BRED INTERNATIONAL, INC., VILNIUS UNIVERSITY
    Inventors: STEPHANE DESCHAMPS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG, MINDAUGAS ZAREMBA
  • Publication number: 20210139874
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.
    Type: Application
    Filed: January 21, 2021
    Publication date: May 13, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ZHENGLIN HOU, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG
  • Publication number: 20210130799
    Abstract: A method of synthetizing cyclic oligoadenylates using a novel catalytic activity of a protein possessing the Palm domain, such as the Cas10 protein, and using such compounds for activation of proteins possessing the CARF doman, such as the Csm6 protein.
    Type: Application
    Filed: May 31, 2018
    Publication date: May 6, 2021
    Inventors: Virginijus SIKSNYS, Migle KAZLAUSKIENE, Georgij KOSTIUK, Gintautas TAMULAITIS
  • Patent number: 10934536
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.
    Type: Grant
    Filed: December 13, 2019
    Date of Patent: March 2, 2021
    Assignee: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Zhenglin Hou, Tautvydas Karvelis, Virginijus Siksnys, Joshua K Young
  • Publication number: 20200190494
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.
    Type: Application
    Filed: December 13, 2019
    Publication date: June 18, 2020
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ZHENGLIN HOU, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG
  • Publication number: 20190264232
    Abstract: Compositions and methods are provided for novel Cas9 orthologs, including, but not limiting to, novel guide polynucleotide/Cas9 endonucleases complexes, single or dual guide RNAs, guide RNA elements, and Cas9 endonucleases. The present disclosure also describes methods for creating a double strand break in a target polynucleotide, methods for genome modification of a target sequence under various in vivo and in vitro conditions, in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell. Also provided are nucleic acid constructs and cells having a modified target site or altered polynucleotide of interest produced by the methods described herein.
    Type: Application
    Filed: February 22, 2019
    Publication date: August 29, 2019
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ZHENGLIN HOU, JOSHUA K. YOUNG, GIEDRIUS GASIUNAS, VIRGINIJUS SIKSNYS
  • Patent number: 10385336
    Abstract: A Type III-A CRISPR-Cas (StCsm) complex of Streptococcus thermophilus comprising crRNA, Csm4, and Csm3 and use for cleavage of RNA bearing a nucleotide sequence complementary to the crRNA, in vitro or in vivo. Methods for site-specific cleavage/shredding of a target RNA molecule using an RNA-guided RNA endonuclease comprising a minimal complex of crRNA, Csm4, and Csm3, and methods of RNA knock-down and RNA knock-out are disclosed.
    Type: Grant
    Filed: March 3, 2017
    Date of Patent: August 20, 2019
    Assignee: VILNIUS UNIVERSITY
    Inventors: Virginijus Siksnys, Migle Kazlauskiene, Gintautas Tamulaitis
  • Publication number: 20190136248
    Abstract: Compositions and methods are provided for novel guide RNA/Cas endonuclease systems. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri MIc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.
    Type: Application
    Filed: May 12, 2016
    Publication date: May 9, 2019
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ANDREW MARK CIGAN, GIEDRIUS GASIUNAS, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG
  • Publication number: 20180346895
    Abstract: Compositions and methods are provided for novel guide RNA/Cas endonuclease systems. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri Mlc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.
    Type: Application
    Filed: July 25, 2018
    Publication date: December 6, 2018
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ANDREW MARK CIGAN, GIEDRIUS GASIUNAS, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K. YOUNG
  • Publication number: 20180258417
    Abstract: Compositions and methods are provided for rapid characterization of Cas endonuclease systems and the elements comprising such systems, including, but not limiting to, rapid characterization of PAM sequences, guide RNA elements and Cas endonucleases. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri MIc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.
    Type: Application
    Filed: May 12, 2016
    Publication date: September 13, 2018
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ANDREW MARK CIGAN, GIEDRIUS GASIUNAS, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG
  • Publication number: 20180187195
    Abstract: Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule in vitro or in vivo using an RNA-guided DNA endonuclease comprising RNA sequences and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro and using a cassette containing a single repeat-spacer-repeat unit.
    Type: Application
    Filed: December 7, 2017
    Publication date: July 5, 2018
    Applicant: VILNIUS UNIVERSITY
    Inventors: Virginijus SIKSNYS, Giedrius GASIUNAS, Tautvydas KARVELIS, Arvydas LUBYS, Lolita ZALIAUSKIENE, Monika GLEMZAITE, Anja SMITH
  • Publication number: 20180105835
    Abstract: Methods and compositions using a CRISPR-Cas Type IIIA resulting in RNA gene knockdown and knockout in an animal.
    Type: Application
    Filed: August 31, 2017
    Publication date: April 19, 2018
    Inventors: Thomas FRICKE, Matthias BOCHTLER, Gintautas TAMULAITIS, Migle KAZLAUSKIENE, Virginijus SIKSNYS
  • Publication number: 20170198286
    Abstract: A Type III-A CRISPR-Cas (StCsm) complex of Streptococcus thermophilus comprising crRNA, Csm4, and Csm3 and use for cleavage of RNA bearing a nucleotide sequence complementary to the crRNA, in vitro or in vivo. Methods for site-specific cleavage/shredding of a target RNA molecule using an RNA-guided RNA endonuclease comprising a minimal complex of crRNA, Csm4, and Csm3, and methods of RNA knock-down and RNA knock-out are disclosed.
    Type: Application
    Filed: March 3, 2017
    Publication date: July 13, 2017
    Inventors: Virginijus Siksnys, Migle Kazlauskiene, Gintautas Tamulaitis
  • Publication number: 20150291961
    Abstract: Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule using an RNA-guided DNA endonuclease comprising at least one RNA sequence and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one pint mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro or using a cassette containing a single repeat-spacer-repeat unit.
    Type: Application
    Filed: June 18, 2015
    Publication date: October 15, 2015
    Inventors: Virginijus SIKSNYS, Giedrius GASIUNAS, Tautvydas KARVELIS
  • Publication number: 20150240261
    Abstract: Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule using an RNA-guided DNA endonuclease comprising at least one RNA sequence and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro or using a cassette containing a single repeat-spacer-repeat unit.
    Type: Application
    Filed: April 10, 2015
    Publication date: August 27, 2015
    Inventors: Virginijus SIKSNYS, Giedrius GASI√úNAS, Tautvydas KARVELIS