Abstract: The present invention relates to a diagnostic device and method for diagnosis thereof for kidney functioning configured for diagnosing one or more ailments associated with the kidneys of a human being. The device comprising of a housing (10A) and a tray (10B). The housing (10A) forms the outer cover in which the tray (10B) is placed. The housing (10A) has a plurality of at least six cavities configured thereon. The tray (10B) is a filter paper printed with a hydrophobic pattern. The device is with the image capturing and image processing unit to process the image to identify the change of colour in reaction zones due to specific biomarkers such as total proteins, creatinine, uric acid, nitrite test, leukocyte esterase (LE), and phosphates. The device is with data interpretation unit to compute the quantitative values.