Abstract: Methods and biosensor systems for improved evaluation of an interaction between an analyte in a fluid sample and a ligand immobilized on a sensor surface of a biosensor are provided. In one example, a method is provided which includes allowing a plurality of fluid samples to flow across a first sensor surface and a second sensor surface having a ligand immobilized thereon, where the fluid samples include a solvent (for example, an organic solvent with bulk effects such as DMSO) at known concentrations. The method further includes creating a data set for each fluid sample and forming a clean data set with outliers removed. Software for performing steps of methods disclosed and a computer readable medium for storing the software are also provided.

Abstract: Methods and biosensor systems for improved evaluation of an interaction between an analyte in a fluid sample and a ligand immobilized on a sensor surface of a biosensor are provided. In one example, a method is provided which includes allowing a plurality of fluid samples to flow across a first sensor surface and a second sensor surface having a ligand immobilized thereon, where the fluid samples include a solvent (for example, an organic solvent with bulk effects such as DMSO) at known concentrations. The method further includes creating a data set for each fluid sample and forming a clean data set with outliers removed. Software for performing steps of methods disclosed and a computer readable medium for storing the software are also provided.