Abstract: A method for performing an assessment related to blood flow in tissue includes receiving fluorescence imaging data associated with movement of a fluorescence imaging agent through a target, determining at least one characteristic associated with a current stage of the movement of the fluorescence imaging agent through the target based on the fluorescence imaging data, comparing the at least one characteristic associated with the current stage to predefined criteria to determine whether the current stage corresponds to a suitable stage for performing an assessment related to blood flow; and, in accordance with the current stage corresponding to the suitable stage, performing the assessment related to blood flow in at least a portion of the target based on the fluorescence imaging data.

Abstract: A method for fluorescence imaging includes receiving first fluorescence imaging data, receiving a user input selecting a reference region associated with the first fluorescence imaging data, determining a reference fluorescence intensity level based on the reference region, receiving second fluorescence imaging data, determining a relative fluorescence intensity level of a region associated with the second fluorescence imaging data that is relative to the reference fluorescence intensity level, and displaying an indication of the relative fluorescence intensity level.