Patents by Inventor Vladimir Georgievich Debabov

Vladimir Georgievich Debabov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for producing web protein, a fused protein, recombinant DNA, an expression vector, a host cell and strain-producers
    Patent number: 9475852
    Abstract: The invention relates to the field of biotechnology and provides a method for producing recombinant proteins from the orb-weaving spider silk in yeast cells. This involves the construction of an expression vector which comprises a DNA sequence encoding a recombinant protein of the orb-weaving spider silk fused with a sequence encoding an ubiquitin-like protein occupying an N-terminal position with respect to the spider silk recombinant protein within the fused protein. The expression of a hybrid gene makes it possible to increase tens of times the production of recombinant spider silk protein, wherein the recombinant protein accumulates in the yeast cells in a water-insoluble fraction in the form of a processed protein free of a hybrid component.
    Type: Grant
    Filed: December 13, 2010
    Date of Patent: October 25, 2016
    Inventors: Vladimir Grigorievich Bogush, Mihail Yurievich Beburov, Vladimir Georgievich Debabov, Dmitriy Georgievich Kozlov, Irek Ilyasovich Gubaydullin, Lyubov Ivanovna Davydova, Igor Arsenievich Zalunin, Konstantin Vasilievich Sidoruk, Sergey Eduardovich Cheperegin
  • METHOD FOR PRODUCING WEB PROTEIN, A FUSED PROTEIN, RECOMBINANT DNA, AN EXPRESSION VECTOR, A HOST CELL AND STRAIN-PRODUCERS
    Publication number: 20140094589
    Abstract: The invention relates to the field of biotechnology and provides a method for producing recombinant proteins from the orb-weaving spider silk in yeast cells. This involves the construction of an expression vector which comprises a DNA sequence encoding a recombinant protein of the orb-weaving spider silk fused with a sequence encoding an ubiquitin-like protein occupying an N-terminal position with respect to the spider silk recombinant protein within the fused protein. The expression of a hybrid gene makes it possible to increase tens of times the production of recombinant spider silk protein, wherein the recombinant protein accumulates in the yeast cells in a water-insoluble fraction in the form of a processed protein free of a hybrid component.
    Type: Application
    Filed: December 13, 2010
    Publication date: April 3, 2014
    Inventors: Vladimir Grigorievich Bogush, Mihail Yurievich Beburov, Vladimir Georgievich Debabov, Dmitriy Georgievich Kozlov, Irek Ilyasovich Gubaydullin, Lyubov Ivanovna Davydova, Igor Arsenievich Zalunin, Konstantin Vasilievich Sidoruk, Sergey Eduardovich Cheperegin
  • Bacterial strain of BKIIM B-3996 as the producer of L-threonine
    Patent number: 7138266
    Abstract: A bacterial strain of Escherichia coli is described which produces L-threonine, and is obtained by a process comprising transduction by bacteriophage P1 which bears a transposon which inactivates threonine dehydrogenase activity, and isolation of a transductant lacking threonine dehydrogenase activity.
    Type: Grant
    Filed: September 2, 2003
    Date of Patent: November 21, 2006
    Assignee: Ajinomoto Co., Inc.
    Inventors: Vladimir Georgievich Debabov, Jury Ivanovich Kozlov, Evgeny Moiseevich Khurges, Vitaly Arkadievich Livshits, Nelli Isaakovna Zhdanova, Mikhail Markovich Gusyatiner, Alexandr Konstantinovich Sokolov, Tatyana Alexandrovna Bachina, Nikolai Kazimirovich Yankovsky, Jury Dmitrievich Tsygankov, Andrei Jurievich Chistoserdov, Tatyana Grigorievna Plotnikova, Irina Olegovna Shakalis, Alla Valentinovna Belareva, Raisa Alexandrovna Arsatiants, Albert Fedorovich Sholin, Tamara Mikhailovna Pozdnyakova
  • Bacterial strain of escherichia coli BKIIM B-3996 as the producer of L-threonine
    Publication number: 20040038380
    Abstract: A bacterial strain of Escherichia coli BKIIM B-3996, a producer of L-threonine, containing a recombinant plasmid pVIC40 and deposited on Nov. 19, 1987 in the collection of microorganism cultures at the USSR Antibiotics Research Institute under Reg. No. 1867.
    Type: Application
    Filed: September 2, 2003
    Publication date: February 26, 2004
    Applicant: Ajinomoto Co., Inc.
    Inventors: Vladimir Georgievich Debabov, Jury Ivanovich Kozlov, Evgeny Moiseevich Khurges, Vitaly Arkadievich Livshits, Nelli Isaakovna Zhdanova, Mikhail Markovich Gusyatiner, Alexandr Konstantinovich Sokolov, Tatyana Alexandrovna Bachina, Nikolai Kazimirovich Yankovsky, Jury Dmitrievich Tsygankov, Andrei Jurievich Chistoserdov, Tatyana Grigorievna Plotnikova, Irina Olegovna Shakalis, Alla Valentinovna Belareva, Raisa Alexandrovna Arsatiants, Albert Fedorovich Sholin, Tamara Mikhailovna Pozdnyakova
  • Bacterial strain of Escherichia coli BKIIM B-3996 as the producer of L-threonine
    Patent number: 6653111
    Abstract: The present invention provides a method for deriving an E. coli strain which produces L-theronine from E.coli strain VNIIgenetika 472T23 by transducing with bacteriophage P1 which bears a transposon.
    Type: Grant
    Filed: July 13, 2001
    Date of Patent: November 25, 2003
    Assignee: Ajinomoto Co., Inc.
    Inventors: Vladimir Georgievich Debabov, Jury Ivanovich Kozlov, Evgeny Moiseevich Khurges, Vitaly Arkadievich Livshits, Nelli Isaakovna Zhdanova, Mikhail Markovich Gusyatiner, Alexandr Konstantinovich Sokolov, Tatyana Alexandrovna Bachina, Nikolai Kazimirovich Yankovsky, Jury Dmitrievich Tsygankov, Andrei Jurievich Chistoserdov, Tatyana Grigorievna Plotnikova, Irina Olegovna Shakalis, Alla Valentinovna Belareva, Raisa Alexandrovna Arsatiants, Albert Fedorovich Sholin, Tamara Mikhailovna Pozdnyakova
  • Bacterial strain of escherichia coli BKIIM B-3996 as the producer of L-threonine
    Publication number: 20010049129
    Abstract: A bacterial strain of Escherichia coli BKIIM B-3996, a producer of L-threonine, containing a recombinant plasmid pVIC40 and deposited on Nov. 19, 1987 in the collection of microorganism cultures at the USSR Antibiotics Research Institute under Reg. No. 1867.
    Type: Application
    Filed: July 13, 2001
    Publication date: December 6, 2001
    Applicant: Ajinomoto Co., Inc.
    Inventors: Vladimir Georgievich Debabov, Jury Ivanovich Kozlov, Evgeny Moiseevich Khurges, Vitaly Arkadievich Livshits, Nelli Isaakovna Zhdanova, Mikhail Markovich Gusyatiner, Alexandr Konstantinovich Sokolov, Tatyana Alexandrovna Bachina, Nikolai Kazimirovich Yankovsky, Jury Dmitrievich Tsygankov, Andrei Jurievich Chistoserdov, Tatyana Grigorievna Plotnikova, Irina Olegovna Shakalis, Alla Valentinovna Belareva, Raisa Alexandrovna Arsatiants, Albert Fedorovich Sholin, Tamara Mikhailovna Pozdnyakova
  • Escherichia coli strain and method for producing L-threonine
    Patent number: 6297031
    Abstract: E. coli strains derived form E. coli strain VNIIgenetika 472t23 and obtained by a process involving transduction by bacteriophage P1 which bears a transposon which inactivates threonine dehydrogenase activity and isolation of a transductant lacking threonine dehydrogenase activity are useful for producing L-threonine.
    Type: Grant
    Filed: June 22, 2000
    Date of Patent: October 2, 2001
    Assignee: Ajinomoto Co., Inc.
    Inventors: Vladimir Georgievich Debabov, Jury Ivancvich Kozlov, Evgeny Mciseevich Khurges, Vitaly Arkadievich Livshits, Nelli Isaakovna Zhdanova, Mikhail Markovich Gusyatiner, Alexandr Konstantinovich Sokolov, Tatyana Alexandrovna Bachina, Nikolai Kazimirovich Yankovsky, Jury Dmitrievich Tsygankov, Andrei Jurievich Christoserdov, Tatyana Grigorievna Plotnikova, Irina Clegovna Shakalis, Alla Valentinovna Belareva, Raisa Alexandrovna Arsatiants, Albert Fedorovich Sholin, Tamara Mikhailovna Pozdnyakova
  • Bacterial strain of Escherichia coli BKIIM B-3996 as the producer of L-threonine
    Patent number: 6165756
    Abstract: A bacterial strain of Escherichia coli BKIIM B-3996, a producer of L-threonine, containing a recombinant plasmid pVIC40 and deposited on Nov. 19, 1987 in the collection of microorganism cultures at the USSR Antiobiotics Research Institute under Reg. No. 1867.
    Type: Grant
    Filed: September 7, 1999
    Date of Patent: December 26, 2000
    Assignee: Ajinomoto Co., Inc.
    Inventors: Vladimir Georgievich Debabov, Jury Ivanovich Kozlov, Evgeny Moiseevich Khurges, Vitaly Arkadievich Livshits, Nelli Isaakovna Zhdanova, Mikhail Markovich Gusyatiner, Alexandr Konstantinovich Sokolov, Tatyana Alexandrovna Bachina, Nikolai Kazimirovich Yankovsky, Jury Dmitrievich Tsygankov, Andrei Jurievich Chistoserdov, Tatyana Grigorievna Plotnikova, Irina Clegovna Shakalis, Alla Valentinovna Belareva, Raisa Alexandrovna Arsatiants, Albert Fedorovich Sholin, Tamara Mikhailovna Pozdnyakova
  • L-threonine-producing microbacteria and a method for the production of L-threonine
    Patent number: 6132999
    Abstract: The present invention provides a novel microbial strain and a method for effectively producing L-threonine. The novel strain can grow on a medium containing molasses, a much cheaper raw material than sucrose. The exemplary novel strain E. coli BKIIM B-5318 bears the plasmid pPRT614, which has threonine biosynthesis genes (thr A, B and C), the expression of which is regulated by a lambda-phage PR promoter and temperature-sensitive C1 repressor. The present microorganism is prototrophic with regard to isoleucine. The strain E. coli BKIIM B-5318 produces more than 70 g/l of L-threonine when cultured at 38-41.degree. C. for 32 hours in a medium containing molasses.
    Type: Grant
    Filed: January 21, 1998
    Date of Patent: October 17, 2000
    Assignee: Ajinomoto Co., Inc.
    Inventors: Vladimir Georgievich Debabov, Uri Ivanovich Kozlov, Evgenyi Moiseevich Khurges, Vytalyi Arkadievich Lifshits, Nelli Isaakovna Zhdanova, Michail Markovich Gusyatiner, Alexander Konstantinovich Sokolov, Tatiana Alexandrovna Bachina, Andrei Yurevich Christoserdov, Uri Dmitrievich Tsigankov, Nikolai Kazimirovich Yankovsky, Sergi Vladimirovich Mashko, Alla Lvovna Lapidus, Oksana Fedorovna Gavrilova, Oleg Alexandrovich Rodionov
  • Bacterial strain of Escherichia coli BKIIM B-3996 as the producer of L-threonine
    Patent number: 5976843
    Abstract: A bacterial strain of Escherichia coli BKIIM B-3996, a producer of L-threonine, containing a recombinant plasmid pVIC40 and deposited on Nov. 19, 1987 in the collection of microorganism cultures at the USSR Antibiotics Research Institute under Reg. No. 1867.
    Type: Grant
    Filed: July 9, 1997
    Date of Patent: November 2, 1999
    Assignee: Ajinomoto Co., Inc.
    Inventors: Vladimir Georgievich Debabov, Jury Ivanovich Kozlov, Evgeny Moiseevich Khurges, Vitaly Arkadievich Livshits, Nelli Isaakovna Zhdanova, Mikhail Markovich Gusyatiner, Alexandr Konstantinovich Sokolov, Tatyana Alexandrovna Bachina, Nikolai Kazimirovich Yankovsky, Jury Dmitrievich Tsygankov, Andrei Jurievich Chistoserdov, Tatyana Grigorievna Plotnikova, Irina Olegovna Shakalis, Alla Valentinovna Belareva, Raisa Alexandrovna Arsatiants, Albert Fedorovich Sholin, Tamara Mikhailovna Pozdnyakova
  • Strain of Rhodococcus rhodochrous as a producer of nitrile hydratase
    Patent number: 5827699
    Abstract: This invention provides a new strain Rhodococcus rhodochrous having a high nitrile hydratase activity and capable of hydrating aliphatic and aromatic nitriles to corresponding amides. An isolated culture of Rhodococcus rhodochrous VKM Ac-1515D is also disclosed for use in the production of nitrile hydratase. An enzymatic inducer is not required in the growth medium, however, the growth medium does include a salt, a carbon source, and a nitrogen source.
    Type: Grant
    Filed: August 15, 1995
    Date of Patent: October 27, 1998
    Assignee: Gosudarstvenny. Nauchno-Issledovatelsky Institut Genetiki I Selektsii Promshlennykh Mikroorganizmov
    Inventors: Alexandr Stepanovich Yanenko, Olga Borisovna Astaurova, Sergei Petrovich Voronin, Tatyana Vasilievna Gerasimova, Nikolai Borisovich Kirsanov, Vladimir Nikolaevich Paukov, Inga Nikolaevna Polyakova, Vladimir Georgievich Debabov
  • Bacterial strain of escherichia coli BKIIM B-3996 as the producer of L-threonine
    Patent number: 5705371
    Abstract: A bacterial strain of Escherichia coli BKIIM B-3996, a producer of L-threonine, containing a recombinant plasmid pVIC40 and deposited on Nov. 19, 1987 in the collection of microorganism cultures at the USSR Antibiotics Research Institute under Reg. No. 1867.
    Type: Grant
    Filed: April 16, 1996
    Date of Patent: January 6, 1998
    Assignee: Ajinomoto Co., Inc.
    Inventors: Vladimir Georgievich Debabov, Jury Ivancvich Kozlov, Evgeny Moiseevich Khurges, Vitaly Arkadievich Livshits, Nelli Isaakcvna Zhdanova, Mikhail Markovich Gusyatiner, Alexandr Konstantinovich Sokolov, Tatyana Alexandrovna Bachina, Nikolai Kazimirovich Yankovsky, Jury Dmitrievich Tsygankov, Andrei Jurievich Chistoserdov, Tatyana Grigorievna Plotnikova, Irina Clegovna Shakalis, Alla Valentinovna Belareva, Raisa Alexandrovna Arsatiants, Albert Fedorovich Sholin, Tamara Mikhailcvna Pozdnyakova
  • Strains of Escherichia coli which produce isoleucine or valine and a method for their production
    Patent number: 5658766
    Abstract: A method for constructing microorganism strains which produce amino acids comprising: combining in one bacterial genome (a) a mutation affecting the aminoacyl-tRNA synthetase corresponding to the selected amino acid, conferring cells auxotrophy which cannot be fully suppressed by addition of usual concentration this amino acid into the culture medium and (b) a mutation which destroys the negative regulation of selected amino acid biosynthesis to yield a strain capable of increased production of the selected amino acid.Strains producing isoleucine and valine.Methods to produce isoleucine and valine by culturing those strains.
    Type: Grant
    Filed: February 20, 1996
    Date of Patent: August 19, 1997
    Assignee: Ajinomoto Co., Inc.
    Inventors: Vitaly Arkadievich Livshits, Vladimir Georgievich Debabov, Aaveilova Oksaua Fedorovva, Zakataeva Natalya Pavlovva, Rustem Saidovich Shakulov, Tatyana Alexandrovna Bachina, Evgeny Moiseevich Khurges