Patents by Inventor Vladimir Knezevic
Vladimir Knezevic has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8779312Abstract: The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.Type: GrantFiled: November 25, 2009Date of Patent: July 15, 2014Assignees: United States of America/NIH, 20/20 Genesystems, Inc.Inventors: Vladimir Knezevic, Dan-Paul Hartmann, Michael R. Emmert-Buck
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Patent number: 7838222Abstract: The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.Type: GrantFiled: March 22, 2006Date of Patent: November 23, 2010Assignees: United States of America/ NIH, 20/20 Genesystems, Inc.Inventors: Vladimir Knezevic, Dan-Paul Hartmann, Michael R. Emmert-Buck
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Publication number: 20100137160Abstract: The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.Type: ApplicationFiled: November 25, 2009Publication date: June 3, 2010Inventors: Vladimir KNEZEVIC, Dan-Paul Hartmann, Michael R. Emmert-Buck
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Publication number: 20060211011Abstract: The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.Type: ApplicationFiled: March 22, 2006Publication date: September 21, 2006Inventors: Vladimir Knezevic, Dan-Paul Hartmann, Michael Emmert-Buck
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Patent number: 6969615Abstract: The present disclosure is directed to devices, arrays, kits and methods for detecting biomolecules in a tissue section (such as a fresh or archival sample, tissue microarray, or cells harvested by an LCM procedure) or other substantially two-dimensional sample (such as an electrophoretic gel or cDNA microarray) by creating “carbon copies” of the biomolecules eluted from the sample and visualizing the biomolecules on the copies using one or more detector molecules (e.g., antibodies or DNA probes) having specific affinity for the biomolecules of interest. Specific methods are provided for identifying the pattern of biomolecules (e.g., proteins and nucleic acids) in the samples. Other specific methods are provided for the identification and analysis of proteins and other biological molecules produced by cells and/or tissue, especially human cells and/or tissue.Type: GrantFiled: November 20, 2001Date of Patent: November 29, 2005Assignees: 20/20 GeneSystems, Inc., The United States of America as represented by the Department of Health and Human ServicesInventors: Vladimir Knezevic, Michael R. Emmert-Buck, Galina Baibakova, Dan-Paul Hartmann, Stephen M. Hewitt, Capre Denise Mitchell, Kevin Gardner
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Publication number: 20050255473Abstract: The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.Type: ApplicationFiled: August 1, 2003Publication date: November 17, 2005Inventors: Vladimir Knezevic, Dan-Paul Hartmann, Michael Emmert-Buck
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Publication number: 20050238535Abstract: Provided herein are methods for preliminary analysis of suspect samples, which can be used in triaging possible contaminated sites (e.g., sites contaminated or thought to be contaminated by biowarfare agents). In some embodiments, the methods involve testing for the presence of protein in the suspect sample; optionally, the sample can also be tested for the presence of sugar, and/or for pH determination. Specific embodiment methods are carried out in tubes or other reaction vessels, others are carried out in a pad format, and still others are carried out in a test strip format. Kits for carrying out the described methods are also provided.Type: ApplicationFiled: January 27, 2005Publication date: October 27, 2005Inventors: Vladimir Knezevic, Dan-Paul Hartmann, Jonathan Cohen, Elizabeth Marcus, Gerard Troy
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Patent number: 6958242Abstract: Provided herein are methods for preliminary analysis of suspect samples, which can be used in triaging possible contaminated sites (e.g., sites contaminated or thought to be contaminated by biowarfare agents). In some embodiments, the methods involve testing for the presence of protein in the suspect sample; optionally, the sample can also be tested for the presence of sugar, and/or for pH determination. Specific embodiment methods are carried out in tubes or other reaction vessels, others are carried out in a pad format, and still others are carried out in a test strip format. Kits for carrying out the described methods are also provided.Type: GrantFiled: May 14, 2004Date of Patent: October 25, 2005Assignee: 20/20/GeneSystems, Inc.Inventors: Vladimir Knezevic, Dan-Paul Hartmann, Jonathan Cohen, Elizabeth Marcus
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Publication number: 20050148090Abstract: Provided herein are methods for preliminary analysis of suspect samples, which can be used in triaging possible contaminated sites (e.g., sites contaminated or thought to be contaminated by biowarfare agents). In some embodiments, the methods involve testing for the presence of protein in the suspect sample; optionally, the sample can also be tested for the presence of sugar, and/or for pH determination. Specific embodiment methods are carried out in tubes or other reaction vessels, others are carried out in a pad format, and still others are carried out in a test strip format. Kits for carrying out the described methods are also provided.Type: ApplicationFiled: May 14, 2004Publication date: July 7, 2005Inventors: Vladimir Knezevic, Dan-Paul Hartmann, Jonathan Cohen, Elizabeth Marcus
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Patent number: 6770485Abstract: Provided herein are methods for preliminary analysis of suspect samples, which can be used in triaging possible contaminated sites (e.g., sites contaminated or thought to be contaminated by biowarfare agents). In some embodiments, the methods involve testing for the presence of protein in the suspect sample; optionally, the sample can also be tested for the presence of sugar, and/or for pH determination. Specific embodiment methods are carried out in tubes or other reaction vessels, others are carried out in a pad format, and still others are carried out in a test strip format. Kits for carrying out the described methods are also provided.Type: GrantFiled: February 21, 2003Date of Patent: August 3, 2004Assignee: 20/20 GeneSystems, Inc.Inventors: Vladimir Knezevic, Dan-Paul Hartmann, Jonathan Cohen, Elizabeth Marcus
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Publication number: 20040081979Abstract: The present disclosure is directed to devices, arrays, kits and methods for detecting biomolecules in a tissue section (such as a fresh or archival sample, tissue microarray, or cells harvested by an LCM procedure) or other substantially two-dimensional sample (such as an electrophoretic gel or cDNA microarray) by creating “carbon copies” of the biomolecules eluted from the sample and visualizing the biomolecules on the copies using one or more detector molecules (e.g., antibodies or DNA probes) having specific affinity for the biomolecules of interest. Specific methods are provided for identifying the pattern of biomolecules (e.g., proteins and nucleic acids) in the samples. Other specific methods are provided for the identification and analysis of proteins and other biological molecules produced by cells and/or tissue, especially human cells and/or tissue.Type: ApplicationFiled: December 1, 2003Publication date: April 29, 2004Inventors: Vladimir Knezevic, Michael R Emmert-Buck, Galina Baibakova, Dan-Paul Hartmann, Stephen M Hewitt, Capre Denise Mitchell, Kevin Gardner
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Publication number: 20040081987Abstract: The present invention is directed to a device and a method for detecting biomolecules in a tissue section or other two-dimensional sample by creating “carbon copies” of the biomolecules eluted from the sample and visualizing the biomolecules on the copies using antibodies or DNA probes having specific affinity for the biomolecules of interest. Thin membranes in a stacked or layered configuration are applied to the sample, such as a tissue section, and reagents and reaction conditions are provided so that the biomolecules are eluted from the sample and transferred onto each of the stacked membranes thereby producing multiple replicas of the biomolecular content of the sample.Type: ApplicationFiled: July 25, 2003Publication date: April 29, 2004Applicants: 20/20 GeneSystems, Inc., The Govt. of the USA as represented by the Secretary of the Dept. of Health and Human Sevs.Inventors: Vladimir Knezevic, Michael R. Emmert-Buck
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Publication number: 20030180961Abstract: Provided herein are methods for preliminary analysis of suspect samples, which can be used in triaging possible contaminated sites (e.g., sites contaminated or thought to be contaminated by biowarfare agents). In some embodiments, the methods involve testing for the presence of protein in the suspect sample; optionally, the sample can also be tested for the presence of sugar, and/or for pH determination. Specific embodiment methods are carried out in tubes or other reaction vessels, others are carried out in a pad format, and still others are carried out in a test strip format. Kits for carrying out the described methods are also provided.Type: ApplicationFiled: February 21, 2003Publication date: September 25, 2003Applicant: 20/20 GeneSystems, Inc.Inventors: Vladimir Knezevic, Dan-Paul Hartmann, Jonathan Cohen, Elizabeth Marcus
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Patent number: 6602661Abstract: The present invention is directed to a device and a method for detecting biomolecules in a tissue section or other two-dimensional sample by creating “carbon copies” of the biomolecules eluted from the sample and visualizing the biomolecules on the copies using antibodies or DNA probes having specific affinity for the biomolecules of interest. Thin membranes in a stacked or layered configuration are applied to the sample, such as a tissue section, and reagents and reaction conditions are provided so that the biomolecules are eluted from the sample and transferred onto each of the stacked membranes thereby producing multiple replicas of the biomolecular content of the sample.Type: GrantFiled: November 20, 2000Date of Patent: August 5, 2003Assignees: 20/20 GeneSystems, Inc., The United States of America as represented by the Department of Health and Human ServicesInventors: Vladimir Knezevic, Michael R. Emmert-Buck
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Publication number: 20020012920Abstract: The invention relates to method and kits for facilitating the identification and analysis of proteins and other biological molecules produced by cells and/or tissue, especially human cells and/or tissue. The invention employs a plurality of differentially prepared and/or processed membranes which permit the identification and analysis of proteins, even when present in complex mixtures.Type: ApplicationFiled: January 4, 2001Publication date: January 31, 2002Inventors: Kevin Gardner, Michael Emmert-Buck, Vladimir Knezevic