Abstract: The technology provided herein relates to novel variants of DNA-Polymerases exhibiting high termo-stability as well as a strong strand displacement activity; to nucleic acid molecules encoding said DNA-Polymerases, vectors, host cells containing the nucleic acids and methods for preparation and producing such enzymes; compositions comprising at least one of the DNA-Polymerases; and methods for using such enzymes in DNA sequencing and/or DNA amplification processes.

Abstract: The technology provided herein relates to novel variants of DNA-Polymerases exhibiting high termo-stability as well as a strong strand displacement activity; to nucleic acid molecules encoding said DNA-Polymerases, vectors, host cells containing the nucleic acids and methods for preparation and producing such enzymes; compositions comprising at least one of the DNA-Polymerases; and methods for using such enzymes in DNA sequencing and/or DNA amplification processes.

Abstract: The present invention provides a chimeric thermostable DNA polymerase that includes a region from a Tth DNA polymerase I, a region from a Taq DNA polymerase I and a DNA polymerase domain. The DNA polymerase domain comprises a portion of a DNA polymerase domain from the Tth DNA polymerase I operably linked to a portion of a DNA polymerase domain from the Taq DNA polymerase I. Also provided are a nucleic acid sequence and an amino acid sequence of the chimeric thermostable enzyme of the invention. The chimeric DNA polymerase enzyme of the invention is useful in DNA amplification reactions such as the polymerase chain reaction.

Abstract: The invention relates to a method of enhancing a DNA polymerase reaction by including in a reaction mixture containing a DNA polymerase a protein of DNA ligase.

Abstract: Compositions and reaction kits are provided for controlling an enzymatic process. The compositions and kits include a substantially water-insoluble, water-permeable polymer and a poorly water-soluble salt of an essential ionic enzymatic reactant. The disclosed compositions and methods are particularly useful for improving the specificity and performance of PCR.

Abstract: Compositions and reaction kits are provided for controlling an enzymatic process. The compositions and kits include a substantially water-insoluble, water-permeable polymer and a poorly water-soluble salt of an essential ionic enzymatic reactant. The disclosed compositions and methods are particularly useful for improving the specificity and performance of PCR.

Abstract: The present invention provides processes for controlling the start of an enzymatic reaction, which is catalysed by a metal ion dependent enzyme. The required metal ion is generated by a redox reaction initiated by heating a metal compound having a metal atom or metal ion with a redox agent. Also provided are kits for controlling the start of an enzymatic reaction. The processes and kits of the invention are useful for improving the specificity and performance of PCR.

Abstract: Compositions and reaction kits are provided for controlling an enzymatic process. The compositions and kits include a substantially water-insoluble, water-permeable polymer and a poorly water-soluble salt of an essential ionic enzymatic reactant. The disclosed compositions and methods are particularly useful for improving the specificity and performance of PCR.