Patents by Inventor Vladimir L. Florentiev

Vladimir L. Florentiev has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 5981734
    Abstract: A method for labeling oligonucleotide molecules, and for immobilizing oligonucleotide and DNA molecules is provided comprising modifying the molecules to create a chemically active group, and contacting activated fluorescent dyes to the region. A method for preparing an immobilization substrate is also provided comprising modifying a gel to contain desired functional groups which covalently interact with certain moieties of the oligonucleotide molecules. A method for immobilizing biomolecules and other molecules within a gel by copolymerization of allyl-substituted oligonucleotides, DNA and proteins with acrylamide is also provided.
    Type: Grant
    Filed: July 17, 1997
    Date of Patent: November 9, 1999
    Assignee: University of Chicago
    Inventors: Andrei Darievich Mirzabekov, Dimitri Y. Proudnikov, Edward N. Timofeev, Svetlana V. Kochetkova, Vladimir L. Florentiev, Valentine V. Shick
  • Patent number: 5552270
    Abstract: A method for sequencing DNA by hybridization that includes the following steps: forming an array of oligonucleotides at such concentrations that either ensure the same dissociation temperature for all fully complementary duplexes or allows hybridization and washing of such duplexes to be conducted at the same temperature; hybridizing said oligonucleotide array with labeled test DNA; washing in duplex dissociation conditions; identifying single-base substitutions in the test DNA by analyzing the distribution of the dissociation temperatures and reconstructing the DNA nucleotide sequence based on the above analysis. A device for carrying out the method comprises a solid substrate and a matrix rigidly bound to the substrate. The matrix contains the oligonucleotide array and consists of a multiplicity of gel portions. Each gel portion contains one oligonucleotide of desired length. The gel portions are separated from one another by interstices and have a thickness not exceeding 30 .mu.m.
    Type: Grant
    Filed: November 9, 1992
    Date of Patent: September 3, 1996
    Assignee: Institut Molekulyarnoi Biologii Imeni V.A.
    Inventors: Konstantin R. Khrapko, Alexandr A. Khorlin, Igor B. Ivanov, Gennady M. Ershov, Jury P. Lysov, Vladimir L. Florentiev, Andrei D. Mirzabekov