Patents by Inventor Vladimir R. Muzykantov

Vladimir R. Muzykantov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20170296675
    Abstract: The invention provides a method for delivering a cargo molecule into a cell using a targeted DNA-based carrier (e.g., DNA dendrimer). Compositions and kits useful in the practice of the methods are also provided.
    Type: Application
    Filed: June 15, 2017
    Publication date: October 19, 2017
    Inventors: Silvia Muro-Galindo, Vladimir R. Muzykantov
  • Publication number: 20170218378
    Abstract: The present invention relates to compositions and methods for delivering lysosomal proteins. The compositions and methods described herein permit the targeted delivery of exogenous lysosomal proteins to cell surface proteins that allow their internalization via non-clathrin pathways. The present invention further relates to the use of the compositions and methods for enzyme replacement therapy of lysosomal storage diseases. Nucleic acids, recombinant cells and kits useful for making and using the compositions of the invention are also provided.
    Type: Application
    Filed: April 18, 2017
    Publication date: August 3, 2017
    Inventors: Silvia MURO GALINDO, Vladimir R. MUZYKANTOV, Edward Howard SCHUCHMAN
  • Patent number: 9707299
    Abstract: The invention provides a method for delivering a cargo molecule into a cell using a targeted DNA-based carrier (e.g., DNA dendrimer). Compositions and kits useful in the practice of the methods are also provided.
    Type: Grant
    Filed: May 22, 2008
    Date of Patent: July 18, 2017
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Silvia Muro-Galindo, Vladimir R. Muzykantov
  • Patent number: 9655953
    Abstract: The present invention relates to compositions and methods for delivering lysosomal proteins. The compositions and methods described herein permit the targeted delivery of exogenous lysosomal proteins to cell surface proteins that allow their internalization via non-clathrin pathways. The present invention further relates to the use of the compositions and methods for enzyme replacement therapy of lysosomal storage diseases. Nucleic acids, recombinant cells and kits useful for making and using the compositions of the invention are also provided.
    Type: Grant
    Filed: October 16, 2014
    Date of Patent: May 23, 2017
    Assignees: Icahn School of Medicine at Mount Sinai, The Trustees of the University of Pennsylvania
    Inventors: Silvia Muro Galindo, Vladimir R. Muzykantov, Edward Howard Schuchman
  • Publication number: 20150132368
    Abstract: The present invention relates to compositions and methods for delivering lysosomal proteins. The compositions and methods described herein permit the targeted delivery of exogenous lysosomal proteins to cell surface proteins that allow their internalization via non-clathrin pathways. The present invention further relates to the use 10 of the compositions and methods for enzyme replacement therapy of lysosomal storage diseases. Nucleic acids, recombinant cells and kits useful for making and using the compositions of the invention are also provided.
    Type: Application
    Filed: October 16, 2014
    Publication date: May 14, 2015
    Inventors: Silvia Muro Galindo, Vladimir R. Muzykantov, Edward Howard Schuchman
  • Patent number: 8889127
    Abstract: The present invention relates to compositions and methods for delivering lysosomal proteins. The compositions and methods described herein permit the targeted delivery of exogenous lysosomal proteins to cell surface proteins that allow their internalization via non-clathrin pathways. The present invention further relates to the use of the compositions and methods for enzyme replacement therapy of lysosomal storage diseases. Nucleic acids, recombinant cells and kits useful for making and using the compositions of the invention are also provided.
    Type: Grant
    Filed: July 1, 2005
    Date of Patent: November 18, 2014
    Assignee: Icahn School of Medicine at Mount Sinai
    Inventors: Silvia Muro Galindo, Vladimir R. Muzykantov, Edward Howard Schuchman
  • Patent number: 8568786
    Abstract: A method of controlling a physical characteristic of polymeric nanocarrier-encapsulated protein particles includes altering or selecting a weight percentage of a hydrophobic polymer block in a total amphiphilic diblock copolymer of a primary emulsion of a double emulsion, freeze-thaw technique. The primary emulsion is formed using a freeze-thaw cycle of the amphiphilic diblock copolymer and a protein having a molecular weight of up to or equal to 300,000 Da. Selection of the hydrophobic polymer block percentage alters one or more characteristics of the resulting nanoparticles, such as shape. Thus, as one aspect, a method of producing filamentous polymeric nanocarrier-encapsulated protein (i.e.
    Type: Grant
    Filed: October 27, 2008
    Date of Patent: October 29, 2013
    Assignee: The Trustees of the Universtiy of Pennsylvania
    Inventors: Eric Simone, Vladimir R. Muzykantov, Thomas D. Dziubla
  • Publication number: 20130058929
    Abstract: Fusion proteins containing a ligand which specifically binds to a selected vascular bed linked to an anti-thrombotic molecule are provided. Also provided are methods for use of these fusion proteins to prevent coagulation, to dissolve blood clots and to protect against the risk of iatrogenic side effects including those arising from cancer therapy and specific vascular occluding agents.
    Type: Application
    Filed: June 20, 2012
    Publication date: March 7, 2013
    Applicant: The Trustees of the University of Pennsylvania
    Inventors: VLADIMIR R. MUZYKANTOV, Claudia Gottstein, Bi-Sen Ding, Douglas B. Cines
  • Patent number: 8333973
    Abstract: Fusion proteins comprising a single chain antigen-binding domain (scFv) of a monoclonal antibody, linked to an anti-thrombotic agent, anti-inflammatory agent, or a pro-drug thereof are provided, where the polypeptide binds to a binding site (antigen) expressed on the surface of a red blood cell at a density greater than 5,000 copies per red blood cell. Pharmaceutical compositions comprising these fusion proteins, and methods of delivering an anti-thrombotic agent to the surface of a red blood cell via delivery of these fusion proteins, and methods of treating or preventing thrombosis, tissue ischemia, acute myocardial infarction (AMI), ischemic stroke, cerebrovascular disease, pulmonary embolism, or ischemic peripheral vascular disease via administration of the fusion proteins or compositions comprising same are also provided.
    Type: Grant
    Filed: December 31, 2008
    Date of Patent: December 18, 2012
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Vladimir R. Muzykantov, Sergei V. Zaitsev, Bi-Sen Ding, Douglas B. Cines
  • Patent number: 8329225
    Abstract: The present invention is a method for encapsulating active protein in a polymeric nanocarrier. The instant method employs homogenization at subzero temperatures so that enzyme activity is retained. Enzymes which can be encapsulated by the present method include, for example, antioxidant enzymes and xenobiotic detoxifying enzymes. Encapsulation of an enzyme protects it from protease degradation and increases therapeutic half-life. Advantageously, polymeric nanoparticles of the invention are permeable to enzyme substrates and therefore enzymes encapsulated by the instant method can exert their effect without release from the nanocarrier. Methods for decomposing a reactive oxygen species, protecting against vascular oxidative stress, and detoxifying a xenobiotic are also provided.
    Type: Grant
    Filed: June 10, 2009
    Date of Patent: December 11, 2012
    Assignee: Trustees of the University of Pennsylvania
    Inventors: Vladimir R. Muzykantov, Thomas D. Dziubla
  • Publication number: 20110262466
    Abstract: Compositions are provided comprising a thrombomodulin domain linked to a targeting moiety that binds to a determinant on the surface of a target endothelial cell or red blood cell, wherein the thrombomodulin domain may be the extracellular domain, the N-terminal lectin-like domain, or an epidermal growth factor (EGF)-like domain. The targeting moiety may be a single chain antigen-binding domain (scFv), and the targeting moiety and thrombomodulin domain of the composition may be linked as a continuous polypeptide chain. Methods of delivery and use of a composition described herein are provided, as well as methods of treating or preventing thrombosis, inflammation, tissue ischemia, sepsis, acute lung injury (ALI), acute myocardial infarction (AMI), ischemic stroke, cerebrovascular disease, pulmonary embolism, or ischemic peripheral vascular disease is provided.
    Type: Application
    Filed: October 16, 2009
    Publication date: October 27, 2011
    Applicant: The Trustees of the University of Pennsylvania
    Inventors: Vladimir R. Muzykantov, Bi-Sen Ding, Douglas B. Cines, Claudia Gottstein, Steven M. Albelda
  • Patent number: 7927629
    Abstract: A method of controlling a physical characteristic of polymeric nanocarrier-encapsulated protein particles includes altering or selecting a weight percentage of a hydrophobic polymer block in a total amphiphilic diblock copolymer of a primary emulsion of a double emulsion, freeze-thaw technique. The primary emulsion is formed using a freeze-thaw cycle of the amphiphilic diblock copolymer and a protein having a molecular weight of up to or equal to 300,000 Da. Selection of the hydrophobic polymer block percentage alters one or more characteristics of the resulting nanoparticles, such as shape. Thus, as one aspect, a method of producing filamentous polymeric nanocarrier-encapsulated protein (i.e.
    Type: Grant
    Filed: October 27, 2007
    Date of Patent: April 19, 2011
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Eric Simone, Vladimir R. Muzykantov, Thomas D. Dziubla
  • Publication number: 20100316571
    Abstract: A method of controlling a physical characteristic of polymeric nanocarrier-encapsulated protein particles includes altering or selecting a weight percentage of a hydrophobic polymer block in a total amphiphilic diblock copolymer of a primary emulsion of a double emulsion, freeze-thaw technique. The primary emulsion is formed using a freeze-thaw cycle of the amphiphilic diblock copolymer and a protein having a molecular weight of up to or equal to 300,000 Da. Selection of the hydrophobic polymer block percentage alters one or more characteristics of the resulting nanoparticles, such as shape. Thus, as one aspect, a method of producing filamentous polymeric nanocarrier-encapsulated protein (i.e.
    Type: Application
    Filed: October 27, 2008
    Publication date: December 16, 2010
    Applicant: The Trustees of the University of Pennsylvania
    Inventors: Eric Simone, Vladimir R. Muzykantov, Thomas D. Dziubla
  • Patent number: 7837997
    Abstract: Compositions and methods for prevention and treatment of uncontrolled formation of intravascular fibrin clots are provided wherein fibrinolytic or anticoagulant drugs are biocompatibly coupled to red blood cell carriers.
    Type: Grant
    Filed: December 18, 2006
    Date of Patent: November 23, 2010
    Assignees: Trustees of the University of Pennsylvania, University of Virginia Patent Foundation
    Inventors: Vladimir R. Muzykantov, Abd Al-Roof Higazi, Juan Carlos Murciano, Douglas Cines, Ronald P. Taylor
  • Publication number: 20100285015
    Abstract: Fusion proteins comprising a single chain antigen-binding domain (scFv) of a monoclonal antibody, linked to an anti-thrombotic agent, anti-inflammatory agent, or a pro-drug thereof are provided, where the polypeptide binds to a binding site (antigen) expressed on the surface of a red blood cell at a density greater than 5,000 copies per red blood cell. Pharmaceutical compositions comprising these fusion proteins, and methods of delivering an anti-thrombotic agent to the surface of a red blood cell via delivery of these fusion proteins, and methods of treating or preventing thrombosis, tissue ischemia, acute myocardial infarction (AMI), ischemic stroke, cerebrovascular disease, pulmonary embolism, or ischemic peripheral vascular disease via administration of the fusion proteins or compositions comprising same are also provided.
    Type: Application
    Filed: December 31, 2008
    Publication date: November 11, 2010
    Applicant: The Trustees of the University of Pennsylvania
    Inventors: Vladimir R. Muzykantov, Sergei V. Zaitsev, Bi-Sen Ding, Douglas B. Cines
  • Publication number: 20100151005
    Abstract: The invention provides a method for delivering a cargo molecule into a cell using a targeted DNA-based carrier (e.g., DNA dendrimer). Compositions and kits useful in the practice of the methods are also provided.
    Type: Application
    Filed: May 22, 2008
    Publication date: June 17, 2010
    Inventors: Silvia Muro-Galindo, Vladimir R. Muzykantov
  • Patent number: 7674466
    Abstract: Methods for targeting and prolonging association of a selected drug to the luminal surface of pulmonary vascular endothelium of an animal are provided wherein a selected drug is administered to an animal in combination with a non-internalizable ICAM-1 antibody which binds to an antigen on the luminal surface of the pulmonary vasculature. This method is particularly useful in dissolution of fibrin clots or prevention of the intravascular coagulation in the pulmonary vasculature.
    Type: Grant
    Filed: September 23, 2005
    Date of Patent: March 9, 2010
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Vladimir R. Muzykantov, Juan Carlos Murciano, D. Neil Granger
  • Publication number: 20090258078
    Abstract: The present invention is a method for encapsulating active protein in a polymeric nanocarrier. The instant method employs homogenization at subzero temperatures so that enzyme activity is retained. Enzymes which can be encapsulated by the present method include, for example, antioxidant enzymes and xenobiotic detoxifying enzymes. Encapsulation of an enzyme protects it from protease degradation and increases therapeutic half-life. Advantageously, polymeric nanoparticles of the invention are permeable to enzyme substrates and therefore enzymes encapsulated by the instant method can exert their effect without release from the nanocarrier. Methods for decomposing a reactive oxygen species, protecting against vascular oxidative stress, and detoxifying a xenobiotic are also provided.
    Type: Application
    Filed: June 10, 2009
    Publication date: October 15, 2009
    Applicant: The Trustees of the University of Pennsylvania
    Inventors: Vladimir R. Muzykantov, Thomas D. Dziubla
  • Patent number: 7597907
    Abstract: The present invention is a method for encapsulating active protein in a polymeric nanocarrier. The instant method employs homogenization at subzero temperatures so that enzyme activity is retained. Enzymes which can be encapsulated by the present method include, for example, antioxidant enzymes and xenobiotic detoxifying enzymes. Encapsulation of an enzyme protects it from protease degradation and increases therapeutic half-life. Advantageously, polymeric nanoparticles of the invention are permeable to enzyme substrates and therefore enzymes encapsulated by the instant method can exert their effect without release from the nanocarrier. Methods for decomposing a reactive oxygen species, protecting against vascular oxidative stress, and detoxifying a xenobiotic are also provided.
    Type: Grant
    Filed: November 4, 2005
    Date of Patent: October 6, 2009
    Assignee: Trustees of the University of Pennsylvania
    Inventors: Vladimir R. Muzykantov, Tom Dziubla
  • Publication number: 20090202511
    Abstract: The present invention relates to compositions and methods for delivering lysosomal proteins. The compositions and methods described herein permit the targeted delivery of exogenous lysosomal proteins to cell surface proteins that allow their internalization via non-clathrin pathways. The present invention further relates to the use 10 of the compositions and methods for enzyme replacement therapy of lysosomal storage diseases. Nucleic acids, recombinant cells and kits useful for making and using the compositions of the invention are also provided.
    Type: Application
    Filed: July 1, 2005
    Publication date: August 13, 2009
    Inventors: Silvia Muro Galindo, Vladimir R. Muzykantov, Edward Howard Schuchman