Abstract: The invention relates to a cell culture device for a three-dimensional cell culture with one or more cell culture units, characterized in that a cell culture unit comprises: i. at least one matrix holder, with a central opening for uptake of a matrix; ii. a support, which is fixedly or reversibly connected to the at least one matrix holder; and iii. a well strip, consisting of a vessel with at least one cavity, which can comprise at least one matrix holder up to the upper end of the central opening or more, wherein the matrix holder is vertically oriented.

Abstract: The present invention includes an in-situ method, comprising a) determining a molecule selected from the group consisting of cell surface molecules and extracellular matrix molecules in a two- or three-dimensional cell culture system comprising living cells and cell culture medium, comprising the steps of i) providing an analyte probe consisting of a detection element, which binds the molecule, and one or more identification elements; ii) binding of the analyte probe to the molecule in the cell culture system, wherein the growth ability of the contained living cells is not substantially impaired by this step; iii) optionally removing unbound analyte probes; iv) releasing the analyte probe; v) transferring the analyte probe into a container which differs from the cell culture system; vi) detecting the identification element(s); and b) continuing the cell cultivation in the cell culture system.

Abstract: The present invention includes an in-situ method, comprising a) determining a molecule selected from the group consisting of cell surface molecules and extracellular matrix molecules in a two- or three-dimensional cell culture system comprising living cells and cell culture medium, comprising the steps of i) providing an analyte probe consisting of a detection element, which binds the molecule, and one or more identification elements; ii) binding of the analyte probe to the molecule in the cell culture system, wherein the growth ability of the contained living cells is not substantially impaired by this step; iii) optionally removing unbound analyte probes; iv) releasing the analyte probe; v) transferring the analyte probe into a container which differs from the cell culture system; vi) detecting the identification element(s); and b) continuing the cell cultivation in the cell culture system.

Abstract: The invention relates to a method of in vitro culturing or expanding human or animal tissue (2, 2?). The method comprises: obtaining a sample of human or animal tissue; downsizing tissue (21, 21?) of the sample; generating an assembly by placing the downsized tissue on a scaffold or hydrogel (22, 22?); arranging the assembly inside a culture chamber of a 3D perfusion system (23, 23?); and perfusing the assembly in the 3D perfusion system for a predefined time (24, 24?). The method according to the invention allows for preparing and directly culturing in vitro fresh tissue specimens for a high efficient in vitro culturing using a perfused bioreactor system.

Abstract: The invention relates to a method of in vitro culturing or expanding human or animal tissue (2, 2?). The method comprises: obtaining a sample of human or animal tissue; downsizing tissue (21, 21?) of the sample; generating an assembly by placing the downsized tissue on a scaffold or hydrogel (22, 22?); arranging the assembly inside a culture chamber of a 3D perfusion system (23, 23?); and perfusing the assembly in the 3D perfusion system for a predefined time (24, 24?). The method according to the invention allows for preparing and directly culturing in vitro fresh tissue specimens for a high efficient in vitro culturing using a perfused bioreactor system.