Patents by Inventor W. Kline Bolton

W. Kline Bolton has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Tissue processing for immunofluorescence microscopy
    Patent number: 4971783
    Abstract: A tissue holding and processing technique suitable for immunofluorescence, histochemical, and light microscopic analysis of the same specimen. The tissue is first placed in formol sucrose, and then transferred to gum sucrose. The tissue is subsequently washed in phosphate buffered saline, then acetone-water, then acetone, and finally in xylene. Finally, the tissue is embedded in paraffin. Tissue processed according to the present invention may be stored indefinitely in the gum sucrose or paraffin.
    Type: Grant
    Filed: November 22, 1982
    Date of Patent: November 20, 1990
    Assignee: The University of VA Alumni Patents Foundation
    Inventors: W. Kline Bolton, Robert M. Mesnard