Abstract: A control system for positioning a marker over a pre-existing roadway surface mark. The control system has one or more imagers having a field of view for imaging an area of roadway mark surface encompassing the roadway mark and a computer having a machine learning network to process the roadway mark image and position the marker over the pre-existing roadway mark.

Abstract: Provided herein are polynucleotides encoding chimeric antigen receptors (CARs) comprising a CD19 antigen binding domain that specifically binds to CD19 and is resistant to rituximab binding; and immune cells comprising these CD19-specific CARs, e.g., CAR-T cells. Also provided are methods of making and using these CD19-specific CARs, and immune cells comprising these CD19-specific CARs.

Abstract: The present invention relates to a composition comprising an aqueous dispersion of acrylic polymer particles; paraffin wax; graphite particles; and extender particles. The composition is useful for making coatings with low water pickup for liquid applied sound damping applications.

Abstract: Systems, methods and devices are provided for the automated centrifugal processing of samples. In some embodiments, an integrated fluidic processing cartridge is provided, in which a centrifugation chamber is fluidically interfaced, through a lateral surface thereof, with a microfluidic device, and wherein the integrated fluidic processing cartridge is configured to be inserted into a centrifuge for centrifugation. A cartridge interfacing assembly may be employed to interface with the integrated fluidic processing cartridge far performing various fluidic processing steps, such as controlling the flow of fluids into and out of the centrifugation chamber, and controlling the flow of fluids into the microfluidic device, and optionally for the further fluidic processing of fluids extracted to the microfluidic device.

Abstract: Systems, methods and devices are provided for the automated centrifugal processing of samples. In some embodiments, an integrated fluidic processing cartridge is provided, in which a centrifugation chamber is fluidically interfaced, through a lateral surface thereof, with a microfluidic device, and wherein the integrated fluidic processing cartridge is configured to be inserted into a centrifuge for centrifugation. A cartridge interfacing assembly may be employed to interface with the integrated fluidic processing cartridge for performing various fluidic processing steps, such as controlling the flow of fluids into and out of the centrifugation chamber, and controlling the flow of fluids into the microfluidic device, and optionally for the further fluidic processing of fluids extracted to the microfluidic device.

Abstract: A method for mixing, milling, and coating a plurality of constituents comprises placing the constituents in a container that includes a cylindrical inner surface; applying a first vibration to the container such that a motion of the vibration is parallel to a longitudinal axis of the container; and applying a second vibration to the container such that the motion of the vibration is not parallel to the longitudinal axis of the container.

Abstract: The present invention relates to a waterborne composition comprising an aqueous dispersion of first and second multistage polymer particles, wherein each of the first and second polymer particles comprises a water-occluded core and a high Tg polymeric shell wherein the second multistage polymer particles further comprise a polymeric binder layer superposing the shell. The composition of the present invention is useful for reducing the load of TiO2 in paint formulations, while producing coatings with excellent hiding and scrub resistance.

Abstract: Provided herein are formulations and drug product processes that can improve cell viability and minimize waste of the manufactured formulated cells for a drug product.

Abstract: The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non Faradaic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradaic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.

Abstract: The present invention relates to a composition comprising an aqueous dispersion of multistage polymer particles comprising a first and a second phase, wherein the first phase comprises structural units of a carboxylic acid monomer or a salt thereof, and a nonionic ethylenically unsaturated monomer; and wherein the second phase comprises a first and second polymer, wherein the first phase or the second phase first polymer or both comprise structural units of a high Tg hydrophobic monomer; and wherein the second phase second polymer comprises at least 80 percent structural units of styrene. The high Tg hydrophobic monomer is defined as being one or more of the following monomers: cyclohexyl methacrylate, isobornyl methacrylate, 4-t-butyl methacrylate, t-butylstyrene, or n-butyl methacrylate. The multistage polymer particles are useful as opaque polymers, which are used in pigmented coating formulations to reduce the load of TiO2.

Abstract: Systems, methods and devices are provided for the automated centrifugal processing of samples. In some embodiments, an integrated fluidic processing cartridge is provided, in which a centrifugation chamber is fluidically interfaced, through a lateral surface thereof, with a microfluidic device, and wherein the integrated fluidic processing cartridge is configured to be inserted into a centrifuge for centrifugation. A cartridge interfacing assembly may be employed to interface with the integrated fluidic processing cartridge for performing various fluidic processing steps, such as controlling the flow of fluids into and out of the centrifugation chamber, and controlling the flow of fluids into the microfluidic device, and optionally for the further fluidic processing of fluids extracted to the micro-fluidic device.

Abstract: Identification and monitoring of healthcare issues includes determining how to process input data based on at least one of identity of a user device that transmits the input data to the system and categorization of the input data. A new record is created in a database of healthcare issues in a healthcare facility where the user device is located when the input data is received. Anonymous reporting of issues is supported by storing an identifier of the user device that transmits the input data to the system, so that the anonymous reporter can be invited by a manager to join in an anonymous chat session. Recording of data during supervisor walkrounds is supported by setting up scheduled walkrounds with default labels to simplify input of identified healthcare issues, while allowing for custom labels to be entered when needed.

Abstract: Systems, methods and devices are provided for the automated centrifugal processing of samples. In some embodiments, an integrated fluidic processing cartridge is provided, in which a centrifugation chamber is fluidically interfaced, through a lateral surface thereof, with a microfluidic device, and wherein the integrated fluidic processing cartridge is configured to be inserted into a centrifuge for centrifugation. A cartridge interfacing assembly may be employed to interface with the integrated fluidic processing cartridge for performing various fluidic processing steps, such as controlling the flow of fluids into and out of the centrifugation chamber, and controlling the flow of fluids into the microfluidic device, and optionally for the further fluidic processing of fluids extracted to the micro-fluidic device.

Abstract: A deployable fiber optic cable for pairing with a connector, the cable including a plurality of partially bonded ribbon fibers each being sized and configured to be rolled into a circular cross section; an elongate member forming a slotted core including a plurality of rounded slots for longitudinally surrounding the circular cross sections of a corresponding plurality of the plurality of partially bonded ribbon fibers; a plurality of rugged fiber tubes each located adjacent a corresponding one of the plurality of slots and wherein each of the plurality of rugged fiber tubes longitudinally surround a corresponding plurality of the plurality of partially bonded ribbon fibers; a plurality of water-blocking yarn members each surrounding a corresponding one of the plurality of rugged fiber tubes; a rugged outer jacket; and a yarn strength member located between the rugged outer jacket and the slotted core.

Abstract: Provided herein are methods for preparing T cells for T cell therapy comprising contacting a cell population at a predetermined cell density, with a concentration of an anti-CD3/CD28 nanomatrix and culturing the cells thereby producing a T cell population comprising an increased percentage of at least one T cell subtype. In some embodiments, the method increases the percentage of stem memory T cells.

Abstract: An auxiliary light system for a tractor for illuminating an area in front of a loader of the tractor. The auxiliary light system can include a pair of light assemblies mounted on respective loader brackets connecting the loader to a main body of the tractor. The light assemblies can be controlled from an operator station of the tractor to move lights on the light assemblies between stowed and operational positions. The light assemblies can be installed as original equipment on the tractor or applied to the tractor as after-market equipment. Associated methods are also disclosed.

Abstract: The present invention relates to a method of preparing an aqueous dispersion of multistage polymer particles comprising contacting under emulsion polymerization conditions and in a staged fashion an aqueous dispersion of carboxylic acid functionalized core polymer particles with first monomers and second monomers. The core comprises structural units of a high Tg hydrophobic monomer and/or the first monomers comprise a high Tg hydrophobic monomer, and the second monomers comprise at least 80 percent styrene. The high Tg hydrophobic monomer is cyclohexyl methacrylate, isobornyl methacrylate, 4-t-butyl methacrylate, t-butylstyrene, or n-butyl methacrylate, or a combination thereof. The multistage polymer particles are useful as opaque polymers, which are used in pigmented coating formulations to reduce the load of TiO2. The particles exhibit excellent collapse resistance and unusually low dry bulk density, and do not require acrylonitrile to achieve this desired combination of properties.

Abstract: A battery system for use in a vehicle is disclosed. The battery system includes a tray having a plurality of battery receptacles. A plurality of batteries are also provided, each battery in the plurality of batteries being received in a battery receptacle. A conductive layer in electrical communication with the plurality of batteries and a terminal for electrically coupling to a vehicle are also provided in the battery system. A cover interlocks with the tray. The cover carries a computer and a diagnostic identification system for the plurality of batteries. A housing for a battery system and a vehicle including a battery system are also disclosed.

Abstract: Provided herein are polynucleotides encoding chimeric antigen receptors (CARs) comprising a CD19 antigen binding domain that specifically binds to CD19 and is resistant to rituximab binding; and immune cells comprising these CD19-specific CARs, e.g., CAR-T cells. Also provided are methods of making and using these CD19-specific CARs, and immune cells comprising these CD19-specific CARs.

Abstract: Provided herein are improved methods for robust TCR+ cell depletion and production of populations of TCR? cells, which can be beneficial to minimize the GvHD risk in patients receiving allogeneic CAR T cell therapy. Provided herein are methods that increase the efficiency of depleting TCR+ cells from a population of cells in order to significantly reduce any residual levels of TCR+ cells present in cell populations in which expression of endogenous TCR has been reduced or eliminated. Associated kits and cell populations are also provided.