Abstract: Disclosed is a cartridge-based detection system to prepare an injected sample to be investigated for determination of a microbial pathogen or another analyte of interest using a detector features a field-safe cartridge. The system features a plurality of reagent chambers located within the cartridge. The system features a rotating cylindrical dial that facilitates a sequential introduction of various fluids contained in each reagent chamber into the dial whereby the fluid washes internally for contact therein. A dial channel is located through the dial. A dual window region of interrogation is centrally located in the dial. The dial is pivoted into position to fluidly connect each mated anterior and posterior reagent chamber via the dial channel. Methods of used are also disclosed.

Abstract: Disclosed is a cartridge-based detection system to prepare an injected sample to be investigated for determination of a microbial pathogen or another analyte of interest using a detector features a field-safe cartridge. The system features a plurality of reagent chambers located within the cartridge. The system features a rotating cylindrical dial that facilitates a sequential introduction of various fluids contained in each reagent chamber into the dial whereby the fluid washes internally for contact therein. A dial channel is located through the dial. A dual window region of interrogation is centrally located in the dial. The dial is pivoted into position to fluidly connect each mated anterior and posterior reagent chamber via the dial channel. Methods of used are also disclosed.

Abstract: The present invention describes a method for the binding of pathogenic microorganisms and their toxic proteins with ligands that have been covalently tethered at some distance from the surface of a substrate: distances of at least fifteen Å are required for microorganism binding ligand tethers and at least six Å are required for protein binding ligand tethers. The ligands described herein include heme compounds, siderophores, polysaccharides, and peptides specific for toxic proteins, outer membrane proteins and conjugated lipids. Non-binding components of the solution to be analyzed are separated from the bound fraction and binding is confirmed by detection of the analyte via microscopy, fluorescence, epifluorescence, luminescence, phosphorescence, radioactivity, or optical absorbance. By patterning numerous ligands in an array on a substrate surface it is possible to taxonomically identify the microorganism by analysis of the binding pattern of the sample to the array.