Abstract: A sampling assembly and a sampling method are provided. A self-calibration unit controls a first switch to be turned on, to enable a first sampling signal to be input to a sampling unit. The sampling unit processes the first sampling signal to obtain a second sampling signal, and outputs the second sampling signal to the self-calibration unit. The self-calibration unit controls the first switch to be turned off, controls a second switch to be turned on, and outputs a first calibration signal to the sampling unit. The sampling unit processes the first calibration signal to obtain a second calibration signal, and outputs the second calibration signal to the self-calibration unit. The self-calibration unit determines an error signal based on the first calibration signal and the second calibration signal. The self-calibration unit obtains a calibrated third sampling signal based on the second sampling signal and the error signal.

Abstract: A biosensing system configured to detect a target DNA and methods of detecting a target DNS are presented. The biosensing system comprises a biosensor comprising a suspension of a hybridization buffer containing dispersed gold nanoparticles, sodium chloride, and single-stranded DNA probes configured to undergo DNA hybridization with the target DNA.

Abstract: A sampling assembly and a sampling method are provided. A self-calibration unit controls a first switch to be turned on, to enable a first sampling signal to be input to a sampling unit. The sampling unit processes the first sampling signal to obtain a second sampling signal, and outputs the second sampling signal to the self-calibration unit. The self-calibration unit controls the first switch to be turned off, controls a second switch to be turned on, and outputs a first calibration signal to the sampling unit. The sampling unit processes the first calibration signal to obtain a second calibration signal, and outputs the second calibration signal to the self-calibration unit. The self-calibration unit determines an error signal based on the first calibration signal and the second calibration signal. The self-calibration unit obtains a calibrated third sampling signal based on the second sampling signal and the error signal.

Abstract: A low-cost photothermal biosensing method and apparatus for the quantitative genetic detection of pathogens such as MTB DNA on a paper hybrid device using a thermometer. First, DNA capture probes were simply immobilized on paper through a one-step surface modification process. After DNA sandwich hybridization, oligonucleotide-functionalized gold nanoparticles (AuNPs) were introduced on paper and then catalyzed the oxidation reaction of 3,3?,5,5?-tetramethylbenzidine (TMB). The produced oxidized TMB, acting as a strong photothermal agent, was used for the photothermal biosensing of MTB DNA under 808 nm laser irradiation. Under optimal conditions, the on-chip quantitative detection of the target DNA was readily achieved using an inexpensive thermometer as a signal recorder. Illustrative embodiments do not require any expensive analytical instrumentation, but can achieve higher sensitivity and there are no color interference issues, compared to conventional colorimetric methods.

Abstract: Embodiments described herein are related to a one-step surface modification method with 3-aminopropyl trimethoxysilane (APTMS), which was developed and applied for DNA immobilization on a paper-based device via the ionic adsorption.

Abstract: A biosensing system configured to detect a target DNA and methods of detecting a target DNS are presented. The biosensing system comprises a biosensor comprising a suspension of a hybridization buffer containing dispersed gold nanoparticles, sodium chloride, and single-stranded DNA probes configured to undergo DNA hybridization with the target DNA.

Abstract: A type of microfluidic platform, photothermal bar-chart chip (PT-Chip), uses on-chip nanomaterial-mediated photothermal effect as a tunable microfluidic driving force to drive ink bar-charts in a visual quantitative readout fashion. The photothermal bar-chart pumping performance can be adjusted remotely by tuning the irradiation parameters, without the need to change any on-chip parameters. The PT-Chip enables a POC visual quantitative diagnostics, by forming nanomaterial-mediated photothermal effects-driven bar-chart microchip for visual quantitative immuno-sensing. In this immunoassay, biomolecules are visually quantified by directly reading the distance that fluids move on the PT-Chip.

Abstract: A network virtualization authority (NVA) device generates a virtual network virtualization edge (NVE) device for members of a link aggregation group and assigns an internet protocol (IP) address to the virtual NVE.

Abstract: A Virtual extensible Local Area Network Tunnel Endpoint (VTEP) receives a multicast group state indicator comprising a state attribute associated with a group identifier of a multicast group, wherein the multicast group is a first multicast group or a second multicast group. The VTEP sets a traffic send and receive state regarding a traffic for a destination VxLAN in the multicast group according to the state attribute for the multicast group in the multicast group state indicator. The traffic is migrated from the first multicast group to the second multicast group.

Abstract: Embodiments described herein are related to a one-step surface modification method with 3-aminopropyl trimethoxysilane (APTMS), which was developed and applied for DNA immobilization on a paper-based device via the ionic adsorption.

Abstract: A method for constructing a network enabling layer-2 interconnection of data centers includes: a current data center registers at a neighbor server when the current data center gets online, obtains information of all registered data centers, generates a virtual bridge port for each registered data center; after the current data center comes online, the current data center obtains information of all newly registered data centers according to a pre-set rule, generates a virtual bridge port for each newly registered data center; establishes a tunnel to each registered data center by using the virtual bridge port corresponding to the registered data center, and transmit data via the tunnel.

Abstract: A Virtual extensible Local Area Network Tunnel Endpoint (VTEP) receives a multicast group state indicator comprising a state attribute associated with a group identifier of a multicast group, wherein the multicast group is a first multicast group or a second multicast group. The VTEP sets a traffic send and receive state regarding a traffic for a destination VxLAN in the multicast group according to the state attribute for the multicast group in the multicast group state indicator. The traffic is migrated from the first multicast group to the second multicast group.

Abstract: A network virtualization authority (NVA) device generates a virtual network virtualization edge (NVE) device for members of a link aggregation group and assigns an internet protocol (IP) address to the virtual NVE.

Abstract: An edge device searches a MAC table to obtain information of a public network egress interface for a packet which is to be sent from a local site to a remote site to access a service, replaces a VLAN ID in the packet with a VLAN ID used for transmission in a public network, and sends the packet in which the VLAN ID has been replaced to a remote site according to the information of the public network egress interface.

Abstract: A method for constructing a network enabling layer-2 interconnection of data centers includes: a current data center registers at a neighbor server when the current data center gets online, obtains information of all registered data centers, generates a virtual bridge port for each registered data center; after the current data center comes online, the current data center obtains information of all newly registered data centers according to a pre-set rule, generates a virtual bridge port for each newly registered data center; establishes a tunnel to each registered data center by using the virtual bridge port corresponding to the registered data center, and transmit data via the tunnel.

Abstract: According to an example, a method for avoiding a loop in a network, which is performed by each ED in each VPN site, comprises: interacting with other EDs in a same VPN site to elect a DED; assigning an authorized forwarding instance to each ED in the same VPN site when the ED is elected as the DED; obtaining the authorized forwarding instance from the DED when the ED is not elected as the DED; allowing traffic of the authorized forwarding instance transmitted from or to a public network, and blocking traffic of unauthorized forwarding instance transmitted from or to the public network.

Abstract: A first BEB device may receive a first LSP packet transmitted from each second BEB device. According to the received first LSP packet, the first BEB device may calculate multicast tunnel information of the service instance from the first BEB device to the second BEB device in the second B-VLAN. The first BEB device may update multicast tunnel information of the service instance in a data plane according to the multicast tunnel information of the service instance from the first BEB device to the second BEB device.

Abstract: A method for constructing a network enabling layer-2 interconnection of data centers includes: a current data center registers at a neighbor server when the current data center gets online, obtains information of all registered data centers, generates a virtual bridge port for each registered data center; after the current data center comes online, the current data center obtains information of all newly registered data centers according to a pre-set rule, generates a virtual bridge port for each newly registered data center; establishes a tunnel to each registered data center by using the virtual bridge port corresponding to the registered data center, and transmit data via the tunnel.

Abstract: A method and an edge device for learning a Medium Access Control (MAC) address are provided. According to an example, based on a request message from a first node to a second node, a session cache item is created in the edge device. The session cache item includes: IP addresses of the first and second nodes, and a first relationship between a MAC address of the first node and an output port corresponding to the first node. Based on a response message from the second node to the first node, the session cache item is updated to include a second relationship between a MAC address of the second node and an output port corresponding to the second node. The first and second relationships are then provided to a data plane of the edge device to facilitate data packet forwarding between the first node and the second node.

Abstract: A method and an edge device for learning a Medium Access Control (MAC) address are provided. According to an example, based on a request message from a first node to a second node, a session cache item is created in the edge device. The session cache item includes: IP addresses of the first and second nodes, and a first relationship between a MAC address of the first node and an output port corresponding to the first node. Based on a response message from the second node to the first node, the session cache item is updated to include a second relationship between a MAC address of the second node and an output port corresponding to the second node. The first and second relationships are then provided to a data plane of the edge device to facilitate data packet forwarding between the first node and the second node.