Patents by Inventor Wanda A. Cromlish

Wanda A. Cromlish has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Intact cell assay for protein tyrosine phosphatases using recombinant baculoviruses
    Patent number: 6329137
    Abstract: The invention relates to an intact cell assay for direct quantitation of protein tyrosine phosphatase (PTP) activity using the baculovirus expression system. A PTP expressed in transformed host insect cells is processed and localized in their predicted subcellular compartments. Assays are performed on the PTP expressing host cells challenged with a substrate such as, p-nitrophenyl phosphate. This substrate is hydrolysed to p-nitrophenol by expressed phosphatase activity. Emergence of p-nitrophenol is determined spectrophotometrically and is a measure of PTP activity. Further, the assay and transformed host cells of this invention are particularly useful in a screening method for selecting potential inhibitors to PTPs. Basically, transformed host cells are pre-incubated with or without inhibitors and the assay conducted as described. This invention relates to an intact cell assay with a direct readout of PTP activity that is useful in a cell-based screening method for selecting inhibitors of PTPs.
    Type: Grant
    Filed: March 7, 2000
    Date of Patent: December 11, 2001
    Assignee: Merck Frosst Canada & Co.
    Inventors: Wanda Cromlish, Brian Kennedy
  • Human cyclooxygenase-2 cDNA and assays for evaluating cyclooxygenase-2 activity
    Patent number: 5543297
    Abstract: The invention discloses a human cyclooxygenase-2 cDNA, a human cyclooxygenase-2 and assays for preferentially and independently measuring cyclogenase-2 or cyclooxygenase-1 activity present in a given sample.
    Type: Grant
    Filed: May 6, 1993
    Date of Patent: August 6, 1996
    Assignee: Merck Frosst Canada, Inc.
    Inventors: Wanda A. Cromlish, Brian P. Kennedy, Gary O'Neill, Philip J. Vickers, Elizabeth Wong, Joseph A. Mancini
  • Human cyclooxygenase-2 cDNA and assays for evaluating cyclooxygenase-2 activity
    Patent number: RE39696
    Abstract: The invention discloses a human cyclooxygenase-2 cDNA, a human cyclooxygenase-2 and assays for preferentially and independently measuring cyclogenase-2 or cyclooxygenase-1 activity present in a given sample.
    Type: Grant
    Filed: November 20, 2000
    Date of Patent: June 19, 2007
    Assignee: Merck Frosst Canada Ltd.
    Inventors: Wanda A. Cromlish, Brian P. Kennedy, Gary O'Neill, Philip J. Vickers, Elizabeth Wong, Joseph A. Mancini