Abstract: In various aspects, the present disclosure provides methods, compositions, reaction mixtures, kits, and systems for preparing nucleic acid libraries, such as for polynucleotide sequencing. In some embodiments, preparation methods comprise tailing reactions, ligation reactions for attaching an adapter, and an amplification reaction between ligation reactions.

Abstract: In various aspects, the present disclosure provides methods, compositions, reaction mixtures, kits, and systems for preparing nucleic acid libraries, such as for polynucleotide sequencing. In some embodiments, preparation methods comprise tailing reactions, ligation reactions for attaching an adapter, and an amplification reaction between ligation reactions.

Abstract: Provided is a second generation sequencing-based method for simultaneously detecting microsatellite locus stability and genomic changes (prettyMSI), especially an application of the detection method in assisting in the diagnosis of patients with colorectal cancer and a corresponding kit. Microsatellite loci are selected from the 22 microsatellite loci as shown in table 1, or any combination of 15, 16, 17, 18, 19, 20, and 21 microsatellite loci of the 22 microsatellite loci.