Abstract: TA preparation method for off-the-shelf human umbilical cord-derived mesenchymal stem cells includes culturing an isolated umbilical cord tissue blocks to obtain P0 cells; carrying out passage on the P0 cells twice to obtain P2 cells, and cryopreserving the P2 cells; the cryopreserved P2 cells are subjected to thawing and passage twice to obtain P4 cells, and the P4 cells are cryopreserved; the cryopreserved P4 cells are subjected to thawing and passage once to obtain P5 cells, and the P5 cells are sequentially digested, washed and resuspended to obtain the human umbilical cord-derived mesenchymal stem cells. The present invention also adopts cryopreservation method to develop a cryopreservation preparation of human umbilical cord-derived mesenchymal stem cells. The cryopreservation preparation is convenient for storage and can be directly administered intravenously after thawing without changing the liquid, avoiding possible contamination risk during dispensing, and is more conducive to clinical use.

Abstract: Disclosed is an application of substituted cinnamamide derivatives in the preparation of anti-anxiety medications, the substituted cinnammide derivatives are compounds having the structure of formula (I) or pharmaceutically acceptable salts thereof, wherein, R1 is —H, —OH, —F, —Cl, —Br, —I, —OCH3, —OCF3, —OCHF2, —OCH2F, —CF3, —CHF2, —CH2F, —CH3, —CH3CH2, —CF3CH2, —CN, —NO2, —NH2 or —COOR5; R2 is H, C1-C10 linear alkyl, C3-C10 branched alkyl, C3-C10 cyclic alkyl, C1-C10 hydroxyalkyl or a N-substituted piperazine-derived group; or R2 is a group forming with adjacent Y a tetrahydropyrrolyl group, a piperidyl group or a cyclohexanimido group.

Abstract: Disclosed is an application of substituted cinnamamide derivatives in the preparation of anti-anxiety medications, the substituted cinnammide derivatives are compounds having the structure of formula (I) or pharmaceutically acceptable salts thereof, wherein, R1 is —H, —OH, —F, —Cl, —Br, —I, —OCH3, —OCF3, —OCHF2, —OCH2F, —CF3, —CHF2, —CH2F, —CH3, —CH3CH2, —CF3CH2, —CN, —NO2, —NH2 or —COOR5; R2 is H, C1-C10 linear alkyl, C3-C10 branched alkyl, C3-C10 cyclic alkyl, C1-C10 hydroxyalkyl or a N-substituted piperazine-derived group; or R2 is a group forming with adjacent Y a tetrahydropyrrolyl group, a piperidyl group or a cyclohexanimido group.

Abstract: The present invention relates to substituted cinnamamide derivatives, the method for preparing thereof and the use thereof. Each of said derivatives has a structure of formula (I). The method for preparing the substituted cinnamamides and their derivatives of the present invention is also disclosed. Substituted piperonal derivatives are selected as starting materials to prepare the substituted cinnamamide derivatives of the present invention by Wittig reaction and acid-amine condensation reaction. Further, a use of the present compounds in preventing and treating depressive-type mental diseases is disclosed.

Abstract: The present invention relates to substituted cinnamamide derivatives, the method for preparing thereof and the use thereof. Each of said derivatives has a structure of formula (I). The method for preparing the substituted cinnamamides and their derivatives of the present invention is also disclosed. Substituted piperonal derivatives are selected as starting materials to prepare the substituted cinnamamide derivatives of the present invention by Wittig reaction and acid-amine condensation reaction. Further, a use of the present compounds in preventing and treating depressive-type mental diseases is disclosed.