Patents by Inventor Wanlin Liu

Wanlin Liu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for enrichment and isolation of endogenous transcription factor and complexes thereof and corresponding tandem arrays of concatenated transcription factor response elements
    Patent number: 8846884
    Abstract: The present invention provides a method for enrichment and isolation of endogenous transcription factors and their complexes. Also, this invention provides corresponding tandem arrays of concatenated transcription factor response elements (catTFRE). The method employs the property of transcription factors binding to sequence-specific DNA elements during regulation of gene expression. The catTFREs are designed and synthesized as concatenate dual copies of DNA response elements for various transcription factors. The DNA sequence of synthesized catTFRE is cloned to a target vector. Biotinylated catTFRE with 200 bp arms is prepared by PCR strategy. For enrichment and isolation of endogenous transcription factors and their complexes, the biotinylated catTFRE is immobilized to streptavidin-coated magnetic beads and then incubated with nuclear extract. Thereby endogenous transcription factors and their complexes are isolated from nuclear extract.
    Type: Grant
    Filed: December 28, 2012
    Date of Patent: September 30, 2014
    Assignees: Baylor College of Medicine, Institute of Radiation Medicine, China Academy of Military Medical Sciences
    Inventors: Jun Qin, Chen Ding, Qiongming Liu, Mingwei Liu, Wanlin Liu, Lei Song