Abstract: The present disclosure related to antisense oligonucleotide capable of specifically targeting the mRNA sequence of a polypyrimidine tract binding protein 1 (PTBP1) and the use thereof. The antisense oligonucleotide can reduce the expression of PTBP1, thereby treating diseases caused by abnormal expression of the PTBP1 gene.

Abstract: Disclosed are a detection method and detection system using suspension liquid biochip detection. The detection method using suspension liquid biochip comprises the following steps: an excitation step and a signal acquisition step. In the excitation step, a laser light emitted by a single laser device is used to excite a classification fluorescence of nanocrystalline fluorescent microspheres and a report fluorescence of an object to be detected; and in the signal acquisition step, fluorescence detection signals are obtained through multiple acquisition channels, respectively. In the present invention, the classification fluorescence in the nanocrystalline fluorescent microspheres and the report fluorescence in the object to be detected in a sample are excited by a single laser device, which is convenient for operation and reduces the production cost of the system, thereby reducing the detection cost.

Abstract: A preparation method of an indium phosphide quantum dot (InP QD) with a large Stokes shift includes: fully dissolving the indium precursor at a first temperature under isolation from oxygen and water, adding a phosphorus precursor, and heating to a second temperature to allow a reaction to obtain an indium phosphide (InP) core; adding a shell precursor to the InP core, and heating to a third temperature to obtain QD with a core-shell structure; and adding anionic and cationic precursors successively to the QD with the core-shell structure at the third temperature to obtain the InP QD with an emission peak adjustable in a range of 465 nm to 650 nm, a high quantum efficiency, and a large Stokes shift. The InP QD mainly has a large Stokes shift to inhibit self-absorption and non-radiative resonance energy transfer.

Abstract: Technologies are provided for generation of exertion-aware paths. Some embodiments include a computing system that can generate a path based at least on first data defining a terrain, second data defining a target level of total work, and third data defining a target level of perceived difficulty. The computing system also can generate a road surface along the path. Generating the road includes embedding metadata defining position-dependent resistance forces based on a physics model, the metadata controlling force feedback at virtual-reality equipment. The computing system also can supply the road surface to the virtual-reality equipment.

Abstract: A method for preparing fluorescent-encoded microspheres coated with metal nanoshells is disclosed herein. By using SPG method, metal nano-material modified with a certain ligand is used as a new surfactant in the emulsification process, and different kinds and different amounts of fluorescent materials are doped into polymer microspheres to prepare fluorescent-encoded microspheres with different fluorescent-encoded signals and uniformly coated metal nanoshells in one step. The prepared fluorescent-encoded microsphere comprises a metal nanoshell, a polymer, and a fluorescent-encoded material. The fluorescent-encoded microsphere has a particle size of 1 ?m˜20 ?m, CV of less than 10%, which can be used for protein/nucleic acid detection.

Abstract: Disclosed are a detection method and detection system using suspension liquid biochip detection. The detection method using suspension liquid biochip comprises the following steps: an excitation step and a signal acquisition step. In the excitation step, a laser light emitted by a single laser device is used to excite a classification fluorescence of nanocrystalline fluorescent microspheres and a report fluorescence of an object to be detected; and in the signal acquisition step, fluorescence detection signals are obtained through multiple acquisition channels, respectively. In the present invention, the classification fluorescence in the nanocrystalline fluorescent microspheres and the report fluorescence in the object to be detected in a sample are excited by a single laser device, which is convenient for operation and reduces the production cost of the system, thereby reducing the detection cost.

Abstract: Technologies are provided for generation of exertion-aware paths. Some embodiments include a computing system that can generate a path based at least on first data defining a terrain, second data defining a target level of total work, and third data defining a target level of perceived difficulty. The computing system also can generate a road surface along the path. Generating the road includes embedding metadata defining position-dependent resistance forces based on a physics model, the metadata controlling force feedback at virtual-reality equipment. The computing system also can supply the road surface to the virtual-reality equipment.

Abstract: A method for preparing fluorescent-encoded microspheres coated with metal nanoshells is disclosed herein. By using SPG method, metal nano-material modified with a certain ligand is used as a new surfactant in the emulsification process, and different kinds and different amounts of fluorescent materials are doped into polymer microspheres to prepare fluorescent-encoded microspheres with different fluorescent-encoded signals and uniformly coated metal nanoshells in one step. The prepared fluorescent-encoded microsphere comprises a metal nanoshell, a polymer, and a fluorescent-encoded material. The fluorescent-encoded microsphere has a particle size of 1 ?m˜20 ?m, CV of less than 10%, which can be used for protein/nucleic acid detection.