Patents by Inventor Wanxia GAI

Wanxia GAI has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240384334
    Abstract: Methods and systems are described herein that include using multiplexed digital amplification reactions to measure the sizes of a plurality of nucleic acid molecules. In various embodiments, the digital amplification reactions use multiple pairs of separate forward and reverse primer pairs to amplify targeted regions of the nucleic acid molecules, where the targeted regions are separated by a specified number of base pairs. In other embodiments, the digital amplification reactions use multiple primer pairs that share a common pair to amplify targeted regions that overlap with one another. The methods and systems are particularly useful for determining the size distributions of the nucleic acid molecules, or classifying a pathology of a subject from whom the nucleic acid molecules are sampled. Advantageously, the methods and systems can measure the sizes of long nucleic acid molecules without relying on inefficient amplification of very long amplicons.
    Type: Application
    Filed: May 8, 2024
    Publication date: November 21, 2024
    Inventors: Yuk-Ming Dennis Lo, Kwan Chee Chan, Peiyong Jiang, Wanxia Gai, Cheuk Yin Yu
  • Publication number: 20240191282
    Abstract: Provided herein are compositions comprising tissue-specific markers for identifying a tissue of origin of a cell-free nucleic acid, e.g., a cell-free DNA molecule. Also provided herein are methods, compositions, and systems for identifying a tissue of origin of a cell-free nucleic acid by determining an absolute amount of cell-free nucleic acids comprising the tissue-specific marker. Also provided herein are methods, compositions, and systems for detecting a cancer in a tissue of an organism by analyzing tissue-specific markers.
    Type: Application
    Filed: December 13, 2023
    Publication date: June 13, 2024
    Inventors: Yuk-Ming Dennis LO, Rossa Wai Kwun CHIU, Kwan Chee CHAN, Wanxia GAI, Lu JI
  • Patent number: 11999950
    Abstract: Particular forward and reverse primers may be used to link distant regions of the same large DNA molecule into a smaller DNA molecule. A reverse primer R1 can have a first portion complementary to an ending sequence of region A and can have a second portion having an overlapping sequence. A forward primer F2 can have a first portion complementary to a starting sequence of region B, where the forward primer includes a complementary overlapping sequence (e.g., the same first portion or a second portion) that is complementary to the overlapping sequence. The first portion of F2 may be the entire primer. The smaller DNA molecules can be used to determine haplotypes of regions. Kits including the particular forward and reverse primers are also described.
    Type: Grant
    Filed: May 11, 2020
    Date of Patent: June 4, 2024
    Assignee: The Chinese University of Hong Kong
    Inventors: Kwan Chee Chan, Wanxia Gai, Yuk-Ming Dennis Lo
  • Publication number: 20240141333
    Abstract: Particular forward and reverse primers may be used to link distant regions of the same large DNA molecule into a smaller DNA molecule. A reverse primer R1 can have a first portion complementary to an ending sequence of region A and can have a second portion having an overlapping sequence. A forward primer F2 can have a first portion complementary to a starting sequence of region B, where the forward primer includes a complementary overlapping sequence (e.g., the same first portion or a second portion) that is complementary to the overlapping sequence. The first portion of F2 may be the entire primer. The smaller DNA molecules can be used to determine haplotypes of regions. Kits including the particular forward and reverse primers are also described.
    Type: Application
    Filed: June 22, 2023
    Publication date: May 2, 2024
    Inventors: Kwan Chee Chan, Wanxia Gai, Yuk-Ming Dennis Lo
  • Patent number: 11884966
    Abstract: Provided herein are compositions comprising tissue-specific markers for identifying a tissue of origin of a cell-free nucleic acid, e.g., a cell-free DNA molecule. Also provided herein are methods, compositions, and systems for identifying a tissue of origin of a cell-free nucleic acid by determining an absolute amount of cell-free nucleic acids comprising the tissue-specific marker. Also provided herein are methods, compositions, and systems for detecting a cancer in a tissue of an organism by analyzing tissue-specific markers.
    Type: Grant
    Filed: March 15, 2019
    Date of Patent: January 30, 2024
    Assignee: GRAIL, LLC
    Inventors: Yuk-Ming Dennis Lo, Rossa Wai Kwun Chiu, Kwan Chee Chan, Wanxia Gai, Lu Ji
  • Patent number: 11718850
    Abstract: Particular forward and reverse primers may be used to link distant regions of the same large DNA molecule into a smaller DNA molecule. A reverse primer R1 can have a first portion complementary to an ending sequence of region A and can have a second portion having an overlapping sequence. A forward primer F2 can have a first portion complementary to a starting sequence of region B, where the forward primer includes a complementary overlapping sequence (e.g., the same first portion or a second portion) that is complementary to the overlapping sequence. The first portion of F2 may be the entire primer. The smaller DNA molecules can be used to determine haplotypes of regions. Kits including the particular forward and reverse primers are also described.
    Type: Grant
    Filed: May 11, 2020
    Date of Patent: August 8, 2023
    Assignee: The Chinese University of Hong Kong
    Inventors: Kwan Chee Chan, Wanxia Gai, Yuk-Ming Dennis Lo
  • Publication number: 20200354785
    Abstract: Particular forward and reverse primers may be used to link distant regions of the same large DNA molecule into a smaller DNA molecule. A reverse primer R1 can have a first portion complementary to an ending sequence of region A and can have a second portion having an overlapping sequence. A forward primer F2 can have a first portion complementary to a starting sequence of region B, where the forward primer includes a complementary overlapping sequence (e.g., the same first portion or a second portion) that is complementary to the overlapping sequence. The first portion of F2 may be the entire primer. The smaller DNA molecules can be used to determine haplotypes of regions. Kits including the particular forward and reverse primers are also described.
    Type: Application
    Filed: May 11, 2020
    Publication date: November 12, 2020
    Inventors: Kwan Chee Chan, Wanxia Gai, Yuk-Ming Dennis Lo
  • Publication number: 20190390257
    Abstract: Provided herein are compositions comprising tissue-specific markers for identifying a tissue of origin of a cell-free nucleic acid, e.g., a cell-free DNA molecule. Also provided herein are methods, compositions, and systems for identifying a tissue of origin of a cell-free nucleic acid by determining an absolute amount of cell-free nucleic acids comprising the tissue-specific marker. Also provided herein are methods, compositions, and systems for detecting a cancer in a tissue of an organism by analyzing tissue-specific markers.
    Type: Application
    Filed: March 15, 2019
    Publication date: December 26, 2019
    Inventors: Yuk-Ming Dennis LO, Rossa Wai Kwun CHIU, Kwan Chee CHAN, Wanxia GAI, Lu Ji