Patents by Inventor Warren Jackson Pledger

Warren Jackson Pledger has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Electroporation buffer composition and method of use
    Patent number: 8551780
    Abstract: The subject invention concerns an electroporation buffer that allows for enhanced transfection efficiency and cell viability of cells during application of an electric current. Buffers of the invention provide for maximum transfer of target particles into cells while maintaining the health and growth potential of the cell population. Compositions of the invention comprise electroporation buffers of approximately physiological ionic strength and pH, and having serum or purified proteins, such as serum albumin, added thereto. The subject invention is suitable for use with any cell type. The subject invention also concerns methods of electroporation using an electroporation buffer of the invention.
    Type: Grant
    Filed: February 2, 2009
    Date of Patent: October 8, 2013
    Assignees: H. Lee Moffitt Cancer Center & Research Institute, Inc., University of South Florida, Proteacel, LLC
    Inventors: Deepak Kumar Agrawal, Warren Jackson Pledger, Jonathan A. Kluft, Asha Agrawal
  • METHOD FOR IMPROVED TRANSFER OF DNA INTO LIVING CELLS BY ELECTROPORATION
    Publication number: 20090197335
    Abstract: The subject invention concerns an electroporation buffer that allows for enhanced transfection efficiency and cell viability of cells during application of an electric current. Buffers of the invention provide for maximum transfer of target particles into cells while maintaining the health and growth potential of the cell population. Compositions of the invention comprise electroporation buffers of approximately physiological ionic strength and pH, and having serum or purified proteins, such as serum albumin, added thereto. The subject invention is suitable for use with any cell type. The subject invention also concerns methods of electroporation using an electroporation buffer of the invention.
    Type: Application
    Filed: February 2, 2009
    Publication date: August 6, 2009
    Applicant: Moffitt Cancer Center
    Inventors: Deepak Kumar Agrawal, Warren Jackson Pledger, Jonathan A. Kluft, Asha Agrawal