Abstract: The present invention relates to systems and methods for reducing noise in image data. Preferred embodiments relate to methods for analyzing two-photon in vivo imaging of biological systems. With neuronal population imaging with subcellular resolution, this modality offers an approach for gaining a fundamental understanding of brain anatomy and physiology. Analysis of calcium imaging data requires denoising, that is separating the signal from complex physiological noise. To analyze two-photon brain imaging data, for example, harmonic regression plus colored noise model and an efficient cyclic descent algorithm for parameter estimation. This approach reliably separates stimulus-evoked fluorescence response from background activity and noise, assesses goodness of fit, and estimates confidence intervals and signal-to-noise ratio.

Abstract: A tripolar antenna is described having at least two electromagnetic signal transmitting/receiving elements arranged such that their axes of signal transmission/reception sensitivity are not parallel, wherein said elements are provided on or at least partially in a substrate of dielectric material so portions of said at least two elements are coplanar and dielectrically isolated from one another. In a preferred arrangement, two dipoles are provided on the substrate in perpendicular orientation and in the plane containing of the surface of said dielectric material. Most preferably a further third element is provided so as to render the antenna tripolar, said third element comprising a first circular disk element secured to an upper surface of the dielectric material, and a second slightly larger circular disk element, concentrically positioned on the corresponding opposite and lower surface of the dielectric.

Abstract: The present invention relates to systems and methods for reducing noise in image data. Preferred embodiments relate to methods for analyzing two-photon in vivo imaging of biological systems. With neuronal population imaging with subcellular resolution, this modality offers an approach for gaining a fundamental understanding of brain anatomy and physiology. Analysis of calcium imaging data requires denoising, that is separating the signal from complex physiological noise. To analyze two-photon brain imaging data, for example, harmonic regression plus colored noise model and an efficient cyclic descent algorithm for parameter estimation. This approach reliably separates stimulus-evoked fluorescence response from background activity and noise, assesses goodness of fit, and estimates confidence intervals and signal-to-noise ratio.

Abstract: A tripolar antenna is described having at least two electromagnetic signal transmitting/receiving elements arranged such that their axes of signal transmission/reception sensitivity are not parallel, wherein said elements are provided on or at least partially in a substrate of dielectric material so portions of said at least two elements are coplanar and dielectrically isolated from one another. In a preferred arrangement, two dipoles are provided on the substrate in perpendicular orientation and in the plane containing of the surface of said dielectric material. Most preferably a further third element is provided so as to render the antenna tripolar, said third element comprising a first circular disk element secured to an upper surface of the dielectric material, and a second slightly larger circular disk element, concentrically positioned on the corresponding opposite and lower surface of the dielectric.