Patents by Inventor Wataru Mizunashi
Wataru Mizunashi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11667939Abstract: The invention provides a method for producing methacrylyl-CoA that converts 3-hydroxyisobutyryl-CoA into methacrylyl-CoA using an enzyme having dehydratase activity as a method for producing methacrylyl-CoA using an enzyme catalyst. In this production method, conversion rate of 3-hydroxyisobutyryl-CoA into methacrylyl-CoA by the enzyme having dehydratase activity is 50% or higher. In this production method, furthermore, the enzyme having dehydratase activity derives from a microorganism belonging to the genus Pseudomonas or Rhodococcus.Type: GrantFiled: April 10, 2019Date of Patent: June 6, 2023Assignee: Mitsubishi Chemical CorporationInventors: Eiji Sato, Fujio Yu, Eiji Nakajima, Michiko Yamazaki, Wataru Mizunashi
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Patent number: 10907136Abstract: It is intended to provide an efficient method for expressing a protein of interest as an active soluble recombinant protein in a transformant. The present invention provides a method for producing an enzyme having improved activity per recombinant as compared with a reference form, comprising the steps of: (1) preparing recombinants each expressing a mutant having an amino acid sequence derived from the amino acid sequence of the reference form by the substitution of one or more cysteine residues by other amino acid residues; (2) selecting a plurality of mutants that exhibit 50% or more activity per recombinant relative to 100% activity of the reference form; and (3) expressing a mutant in which corresponding amino acid residues are substituted at two or more positions among the respective positions of the substituted amino acid residues of the mutants selected in step (2).Type: GrantFiled: February 28, 2019Date of Patent: February 2, 2021Assignee: Mitsubishi Chemical CorporationInventors: Fujio Yu, Wataru Mizunashi
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Patent number: 10851392Abstract: Provided is a method for producing a methacrylic acid ester using a biocatalyst, said method comprising a step for treating methacrylyl-CoA with an alcohol or phenol in the presence of an alcohol acyltransferase to synthesize the methacrylic acid ester. According to this production method, a methacrylic acid ester can be efficiently produced while largely reducing energy, resource and environmental load, compared with the conventional chemical production processes.Type: GrantFiled: September 10, 2013Date of Patent: December 1, 2020Assignee: Mitsubishi Chemical CorporationInventors: Eiji Sato, Fujio Yu, Wataru Mizunashi, Eiji Nakajima
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Patent number: 10676766Abstract: The invention relates to an engineered eukaryotic microorganism into which a gene encoding an acyl-CoA dehydrogenase is introduced and a method for producing methacrylic acid esters such as MMA and MMA-CoA and precursors thereof using the microorganism.Type: GrantFiled: April 20, 2018Date of Patent: June 9, 2020Assignees: The Regents of the University of California, Mitsubishi Chemical CorporationInventors: Michelle O'Malley, Kevin Solomon, Wataru Mizunashi, Fujio Yu
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Patent number: 10570426Abstract: Provided is a method for producing a methacrylic acid ester using a biocatalyst, said method comprising a step of reacting an alcohol or a phenol with methacrylyl-CoA in the presence of an alcohol acyltransferase originated from a plant selected from the group consisting of a plant belonging to the genus Osmanthus, a plant belonging to the genus Vitis, a plant belonging to the genus Citrus, a plant belonging to the genus Durio, a plant belonging to the genus Magnolia and a plant belonging to the genus Chamaemelum to thereby synthesize the methacrylic acid ester.Type: GrantFiled: March 5, 2015Date of Patent: February 25, 2020Assignee: Mitsubishi Chemical CorporationInventors: Yasuhisa Asano, Eiji Sato, Fujio Yu, Wataru Mizunashi
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Publication number: 20190256880Abstract: The invention provides a method for producing methacrylyl-CoA that converts 3-hydroxyisobutyryl-CoA into methacrylyl-CoA using an enzyme having dehydratase activity as a method for producing methacrylyl-CoA using an enzyme catalyst. In this production method, conversion rate of 3-hydroxyisobutyryl-CoA into methacrylyl-CoA by the enzyme having dehydratase activity is 50% or higher. In this production method, furthermore, the enzyme having dehydratase activity derives from a microorganism belonging to the genus Pseudomonas or Rhodococcus.Type: ApplicationFiled: April 10, 2019Publication date: August 22, 2019Applicant: Mitsubishi Chemical CorporationInventors: Eiji SATO, Fujio YU, Eiji NAKAJIMA, Michiko YAMAZAKI, Wataru MIZUNASHI
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Publication number: 20190185825Abstract: It is intended to provide an efficient method for expressing a protein of interest as an active soluble recombinant protein in a transformant. The present invention provides a method for producing an enzyme having improved activity per recombinant as compared with a reference form, comprising the steps of: (1) preparing recombinants each expressing a mutant having an amino acid sequence derived from the amino acid sequence of the reference form by the substitution of one or more cysteine residues by other amino acid residues; (2) selecting a plurality of mutants that exhibit 50% or more activity per recombinant relative to 100% activity of the reference form; and (3) expressing a mutant in which corresponding amino acid residues are substituted at two or more positions among the respective positions of the substituted amino acid residues of the mutants selected in step (2).Type: ApplicationFiled: February 28, 2019Publication date: June 20, 2019Applicant: Mitsubishi Chemical CorporationInventors: Fujio YU, Wataru Mizunashi
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Patent number: 10323264Abstract: The invention provides a method for producing methacrylyl-CoA that converts 3-hydroxyisobutyryl-CoA into methacrylyl-CoA using an enzyme having dehydratase activity as a method for producing methacrylyl-CoA using an enzyme catalyst. In this production method, conversion rate of 3-hydroxyisobutyryl-CoA into methacrylyl-CoA by the enzyme having dehydratase activity is 50% or higher. In this production method, furthermore, the enzyme having dehydratase activity derives from a microorganism belonging to the genus Pseudomonas or Rhodococcus.Type: GrantFiled: July 25, 2014Date of Patent: June 18, 2019Assignee: Mitsubishi Chemical CorporationInventors: Eiji Sato, Fujio Yu, Eiji Nakajima, Michiko Yamazaki, Wataru Mizunashi
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Patent number: 10294500Abstract: To provide a method for directly and efficiently producing methacrylic acid in a single step from renewable raw materials and/or biomass arising from the utilization of the renewable raw materials. Further provided is a method for producing methacrylic acid using microbes having the ability to produce methacrylic acid, from renewable raw materials and/or biomass arising from the utilization of the renewable raw materials, as a carbon source and/or energy source. The method for producing methacrylic acid enables methacrylic acid to be safely and easily produced from biomass, without using petroleum-derived raw materials, by utilizing microbes having the ability to produce methacrylic acid.Type: GrantFiled: September 10, 2013Date of Patent: May 21, 2019Assignee: Mitsubishi Chemical CorporationInventors: Eiji Sato, Michiko Yamazaki, Eiji Nakajima, Fujio Yu, Toshio Fujita, Wataru Mizunashi
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Publication number: 20180346942Abstract: The invention relates to an engineered eukaryotic microorganism into which a gene encoding an acyl-CoA dehydrogenase is introduced and a method for producing methacrylic acid esters such as MMA and MMA-CoA and precursors thereof using the microorganism.Type: ApplicationFiled: April 20, 2018Publication date: December 6, 2018Applicants: The Regents of the University of California, Mitsubishi Chemical CorporationInventors: Michelle O'MALLEY, Kevin SOLOMON, Wataru MIZUNASHI, Fujio YU
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Publication number: 20170022525Abstract: Provided is a method for producing a methacrylic acid ester using a biocatalyst, said method comprising a step of reacting an alcohol or a phenol with methacrylyl-CoA in the presence of an alcohol acyltransferase originated from a plant selected from the group consisting of a plant belonging to the genus Osmanthus, a plant belonging to the genus Vitis, a plant belonging to the genus Citrus, a plant belonging to the genus Durio, a plant belonging to the genus Magnolia and a plant belonging to the genus Chamaemelum to thereby synthesize the methacrylic acid ester.Type: ApplicationFiled: March 5, 2015Publication date: January 26, 2017Applicant: Mitsubishi Rayon Co., Ltd.Inventors: Yasuhisa ASANO, Eiji SATO, Fujio YU, Wataru MIZUNASHI
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Publication number: 20160145665Abstract: The invention provides a method for producing methacrylyl-CoA that converts 3-hydroxyisobutyryl-CoA into methacrylyl-CoA using an enzyme having dehydratase activity as a method for producing methacrylyl-CoA using an enzyme catalyst. In this production method, conversion rate of 3-hydroxyisobutyryl-CoA into methacrylyl-CoA by the enzyme having dehydratase activity is 50% or higher. In this production method, furthermore, the enzyme having dehydratase activity derives from a microorganism belonging to the genus Pseudomonas or Rhodococcus.Type: ApplicationFiled: July 25, 2014Publication date: May 26, 2016Applicant: Mitsubishi Rayon Co., Ltd.Inventors: Eiji SATO, Fujio YU, Eiji NAKAJIMA, Michiko YAMAZAKI, Wataru MIZUNASHI
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Patent number: 9212356Abstract: The present invention provides a modified ethylenediamine-N,N?-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N?-disuccinate:ethylenediamine lyase activity.Type: GrantFiled: April 2, 2014Date of Patent: December 15, 2015Assignee: MITSUBISHI RAYON CO., LTD.Inventors: Takanori Akiyama, Wataru Mizunashi, Tetsuji Nakamura
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Publication number: 20150191756Abstract: To provide a method for directly and efficiently producing methacrylic acid in a single step from renewable raw materials and/or biomass arising from the utilization of the renewable raw materials. Further provided is a method for producing methacrylic acid using microbes having the ability to produce methacrylic acid, from renewable raw materials and/or biomass arising from the utilization of the renewable raw materials, as a carbon source and/or energy source. The method for producing methacrylic acid enables methacrylic acid to be safely and easily produced from biomass, without using petroleum-derived raw materials, by utilizing microbes having the ability to produce methacrylic acid.Type: ApplicationFiled: September 10, 2013Publication date: July 9, 2015Applicant: MITSUBISHI RAYON CO., LTDInventors: Eiji Sato, Michiko Yamazaki, Eiji Nakajima, Fujio Yu, Toshio Fujita, Wataru Mizunashi
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Publication number: 20150184207Abstract: Provided is a method for producing a methacrylic acid ester using a biocatalyst, said method comprising a step for treating methacrylyl-CoA with an alcohol or phenol in the presence of an alcohol acyltransferase to synthesize the methacrylic acid ester. According to this production method, a methacrylic acid ester can be efficiently produced while largely reducing energy, resource and environmental load, compared with the conventional chemical production processes.Type: ApplicationFiled: September 10, 2013Publication date: July 2, 2015Applicant: Mitsubishi Rayon Co., Ltd.Inventors: Eiji Sato, Fujio Yu, Wataru Mizunashi, Eiji Nakajima
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Patent number: 8895285Abstract: The present invention provides a modified ethylenediamine-N,N?-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N?-disuccinate:ethylenediamine lyase activity.Type: GrantFiled: July 12, 2012Date of Patent: November 25, 2014Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Takanori Akiyama, Wataru Mizunashi, Tetsuji Nakamura
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Publication number: 20140248672Abstract: The present invention provides a modified ethylenediamine-N,N?-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N?-disuccinate:ethylenediamine lyase activity.Type: ApplicationFiled: April 2, 2014Publication date: September 4, 2014Applicant: MITSUBISHI RAYON CO., LTD.Inventors: Takanori AKIYAMA, Wataru MIZUNASHI, Tetsuji NAKAMURA
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Patent number: 8728792Abstract: The present invention provides a modified ethylenediamine-N,N?-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N?-disuccinate:ethylenediamine lyase activity.Type: GrantFiled: January 21, 2010Date of Patent: May 20, 2014Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Takanori Akiyama, Wataru Mizunashi, Tetsuji Nakamura
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Publication number: 20130184448Abstract: The present invention provides a modified ethylenediamine-N,N?-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N?-disuccinate:ethylenediamine lyase activity.Type: ApplicationFiled: July 12, 2012Publication date: July 18, 2013Applicant: MITSUBISHI RAYON CO., LTD.Inventors: Takanori AKIYAMA, Wataru MIZUNASHI, Tetsuji NAKAMURA
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Publication number: 20100151537Abstract: The present invention provides a modified ethylenediamine-N,N?-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N?-disuccinate:ethylenediamine lyase activity.Type: ApplicationFiled: January 21, 2010Publication date: June 17, 2010Applicant: Mitsubishi Rayon Co., Ltd.Inventors: Takanori Akiyama, Wataru Mizunashi, Tetsuji Nakamura