Abstract: An information processing device comprises: an image processor which generates surface information from point cloud data generated on the basis of position information of an object, using a value of a first parameter and a value of a second parameter; and a display controller which causes a display to display a surface image on the basis of the generated surface information, wherein the image processor generates a plurality of pieces of surface information, using a plurality of values of the first parameter and a plurality of values of the second parameter, and the display controller causes the display to display a plurality of surface images on the basis of the plurality of pieces of generated surface information.

Abstract: An image-processing device including: a determination unit that determines a captured image on the basis of a ratio of cells and a distribution of the cells in the captured image acquired by imaging a sample including cells; and an area setting unit that sets an area used for analyzing the cells of the sample on the basis of a result of the determination acquired by the determination unit.

Abstract: A microscope apparatus includes an illumination optical system that emits activating light and exciting light, an image capturing unit that captures an image formed by an imaging optical system, an image processing unit that carries out image processing using an image capturing result from the image capturing unit, and a controller. The controller provides a plurality of image generation periods and interrupt periods; causes, in the image generation periods, the activated fluorescent material to be irradiated with the exciting light and causes the image capturing unit to capture an image of the fluorescent light from the activated fluorescent material in a plurality of frame periods; and sets an intensity of the exciting light in the interrupt periods to be lower than an intensity in the image generation periods or causes the emission of the exciting light to stop in the interrupt periods.

Abstract: An information processing device comprises: an image processor which generates surface information from point cloud data generated on the basis of position information of an object, using a value of a first parameter and a value of a second parameter; and a display controller which causes a display to display a surface image on the basis of the generated surface information, wherein the image processor generates a plurality of pieces of surface information, using a plurality of values of the first parameter and a plurality of values of the second parameter, and the display controller causes the display to display a plurality of surface images on the basis of the plurality of pieces of generated surface information.

Abstract: An image-processing device including: a determination unit that determines a captured image on the basis of a ratio of cells and a distribution of the cells in the captured image acquired by imaging a sample including cells; and an area setting unit that sets an area used for analyzing the cells of the sample on the basis of a result of the determination acquired by the determination unit.

Abstract: A microscope, including: an illumination optical system that irradiates a specimen with illumination light from an oblique direction; an observation optical system including an objective lens; and a controller that moves at least one of a stage to hold the specimen and the objective lens in a same direction as an optical axis of the objective lens. The controller changes, when moving at least one of the stage and the objective lens, an incident angle of the illumination light with respect to the specimen.

Abstract: An information processing device includes a machine learner that, in a neural network having an input layer to which data representing an image of fluorescence is input and an output layer that outputs a feature quantity of the image of fluorescence, calculates a coupling coefficient between the input layer and the output layer, using an output value that is output from the output layer when input value teacher data is input to the input layer, and feature quantity teacher data.

Abstract: A microscope, including: an illumination optical system that irradiates a specimen with illumination light from an oblique direction; an observation optical system including an objective lens; and a controller that moves at least one of a stage to hold the specimen and the objective lens in a same direction as an optical axis of the objective lens. The controller changes, when moving at least one of the stage and the objective lens, an incident angle of the illumination light with respect to the specimen.

Abstract: A microscope apparatus includes an illumination optical system that emits activating light and exciting light, an image capturing unit that captures an image formed by an imaging optical system, an image processing unit that carries out image processing using an image capturing result from the image capturing unit, and a controller . The controller provides a plurality of image generation periods and interrupt periods; causes, in the image generation periods, the activated fluorescent material to be irradiated with the exciting light and causes the image capturing unit to capture an image of the fluorescent light from the activated fluorescent material in a plurality of frame periods; and sets an intensity of the exciting light in the interrupt periods to be lower than an intensity in the image generation periods or causes the emission of the exciting light to stop in the interrupt periods.

Abstract: The microscope device includes an imager, an image processor, and a controller. In a first period, the controller causes excitation light to be emitted and causes the imager to image a fluorescent image from an activated fluorescent substance in a plurality of frame periods. In a second period, the controller causes a fiducial marker to be irradiated with auxiliary light and causes the imager to image a fluorescent image from the fiducial marker, causes irradiation with the excitation light in the second period to stop or causes the intensity thereof to be reduced to be lower than that in the first period, and causes irradiation with the auxiliary light in the first period to stop or causes the intensity thereof to be reduced to be lower than that in the second period. The image processor uses an imaging result obtained in the second period to correct at least a part of an imaging result obtained in the first period and uses at least a part of the corrected imaging result to generate one image.

Abstract: The present invention provides an artificial mutant of GFP having a novel emission peak, i.e., a fluorescent protein having an emission peak at 424 nm comprising an amino acid sequence represented by SEQ ID NO: 1, in which each of the amino acid residues at the 66th position and the 175th position is replaced and at least one of the amino acid residues at the 72nd position and the 206th position is further replaced, or a fluorescent protein having an emission peak at 424 nm and a pH-independent fluorescence intensity, in which each of the amino acid residues at the 65th, 145th, 148th, 46th and/or 203rd positions is further substituted. The fluorescent protein of the invention emits fluorescence having an emission peak at 424 nm and can be visually distinguished by its ultramarine color from other fluorescent proteins. The fluorescent protein has a pH-independent fluorescence intensity which is not affected by pH changes.