Patents by Inventor Wayne Barnes

Wayne Barnes has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • T7 expression system
    Patent number: 9163246
    Abstract: The present invention provides an improved prokaryotic cell expression system employing a tightly controlled host strain construct that controls uninduced, leaky expression of proteins while still auto-inducing well. Various aspects of the present invention address and overcome the problem of uninduced basal expression by providing a host strain that comprises a T7 polymerase gene, and mutants thereof, inserted between lac Z and lac Y of the lac operon (a “ZRY” construct), downstream of an otherwise wild-type lac operon control region.
    Type: Grant
    Filed: August 8, 2008
    Date of Patent: October 20, 2015
    Inventor: Wayne Barnes
  • T7 EXPRESSION SYSTEM
    Publication number: 20110300576
    Abstract: The present invention provides an improved prokaryotic cell expression system employing a tightly controlled host strain construct that controls uninduced, leaky expression of proteins while still auto-inducing well. Various aspects of the present invention address and overcome the problem of uninduced basal expression by providing a host strain that comprises a T7 polymerase gene, and mutants thereof, inserted between lac Z and lac Y of the lac operon (a “ZRY” construct), downstream of an otherwise wild-type lac operon control region.
    Type: Application
    Filed: August 8, 2008
    Publication date: December 8, 2011
    Inventor: Wayne Barnes
  • Use of whole blood in PCR reactions
    Publication number: 20060084074
    Abstract: A method of obtaining DNA amplification of a nucleic acid target from a volume of whole blood comprising performing DNA amplification in a PCR assay mixture with a blood-resistant polymerase.
    Type: Application
    Filed: December 6, 2004
    Publication date: April 20, 2006
    Inventors: Milko Kermekchiev, Wayne Barnes
  • Use of whole blood in PCR reactions
    Publication number: 20050260606
    Abstract: A method of obtaining DNA amplification of a nucleic acid target from a volume of whole blood comprising performing DNA amplification in a PCR assay mixture with a blood-resistant polymerase.
    Type: Application
    Filed: May 20, 2004
    Publication date: November 24, 2005
    Inventors: Milko Kermekchiev, Wayne Barnes