Patents by Inventor Wayne Morris Barnes

Wayne Morris Barnes has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Use of whole blood in PCR reactions
    Patent number: 8470563
    Abstract: A method of obtaining DNA amplification of a nucleic acid target from a volume of whole blood comprising performing DNA amplification in a PCR assay mixture with a blood-resistant polymerase.
    Type: Grant
    Filed: December 8, 2008
    Date of Patent: June 25, 2013
    Assignee: DNA Polymerase Technology Inc.
    Inventors: Milko B. Kermekchiev, Wayne Morris Barnes
  • USE OF WHOLE BLOOD IN PCR REACTIONS
    Publication number: 20090170060
    Abstract: A method of obtaining DNA amplification of a nucleic acid target from a volume of whole blood comprising performing DNA amplification in a PCR assay mixture with a blood-resistant polymerase.
    Type: Application
    Filed: December 8, 2008
    Publication date: July 2, 2009
    Applicant: DNA POLYMERASE TECHNOLOGY INC.
    Inventors: Milko B. Kermekchiev, Wayne Morris Barnes
  • Ribocloning: Recombinant DNA Construction using Primers with RIBO Bases
    Publication number: 20080262212
    Abstract: The present invention provides methods of linking nucleic acids without the use of restriction enzymes or any joining enzyme such as ligase. More specifically, the present invention provides methods for cloning or rearranging a double-stranded target DNA which is a PCR product into a double-stranded vector DNA which is a PCR product, said DNAs being amplified using primers that contain at least one ribonucleotide, preferably at or near the respective 3? ends of the primers, such that RNA-specific cleavage, preferably by RNAse A, will allow release of the primers to create long matching 3?-sticky ends.
    Type: Application
    Filed: April 11, 2008
    Publication date: October 23, 2008
    Inventor: Wayne Morris Barnes
  • Ribocloning: recombinant DNA construction using primers with ribo bases
    Patent number: 7393635
    Abstract: The present invention provides methods of linking nucleic acids without the use of restriction enzymes or any joining enzyme such as ligase. More specifically, the present invention provides methods for cloning or rearranging a double-stranded target DNA which is a PCR product into a double-stranded vector DNA which is a PCR product, said DNAs being amplified using primers that contain at least one ribonucleotide, preferably at or near the respective 3? ends of the primers, such that RNA-specific cleavage, preferably by RNAse A, will allow release of the primers to create long matching 3?-sticky ends.
    Type: Grant
    Filed: May 16, 2002
    Date of Patent: July 1, 2008
    Assignee: DNA Polymerase Technology, Inc.
    Inventor: Wayne Morris Barnes
  • Ribocloning: recombinant DNA construction using primers with ribo bases
    Publication number: 20030215924
    Abstract: The present invention provides methods of linking nucleic acids without the use of restriction enzymes or any joining enzyme such as ligase. More specifically, the present invention provides methods for cloning or rearranging a double-stranded target DNA which is a PCR product into a double-stranded vector DNA which is a PCR product, said DNAs being amplified using primers that contain at least one ribonucleotide, preferably at or near the respective 3′ ends of the primers, such that RNA-specific cleavage, preferably by RNAse A, will allow release of the primers to create long matching 3′-sticky ends.
    Type: Application
    Filed: May 16, 2002
    Publication date: November 20, 2003
    Inventor: Wayne Morris Barnes