Abstract: In one embodiment, the present invention relates to a non-enzymatic method for isolating stem cells from adipose tissue, wherein the method comprises treating adipose tissue with ultrasonic cavitation to break up the adipose tissue and lyses mature adipocytes, resulting in a stromal vascular fraction containing viable stromal/stem cells.

Abstract: In one embodiment, the present invention relates to a method for reducing the frequency and/or severity of headache in a subject comprising administering to the subject a composition comprising the stromal vascular fraction or stromal cells of adipose tissue. In another embodiment, the present invention relates to a method for reducing the frequency and/or severity of headache in a subject comprising administering to the subject a composition compositing bone marrow. In another embodiment, the present invention relates to a method for reducing the frequency or severity of headache in a subject comprising administering to the subject a composition comprising adult stem cells.

Abstract: In one embodiment, the present invention relates to a non-enzymatic method for isolating stem cells from adipose tissue, wherein the method comprises treating adipose tissue with ultrasonic cavitation to break up the adipose tissue and lyses mature adipocytes, resulting in a stromal vascular fraction containing viable stromal/stem cells.

Abstract: In one embodiment, the present invention relates to a method for treating tinnitus in a subject including administering to the subject a composition including a stromal vascular fraction from adipose tissue. In another embodiment, the present invention relates to a method for treating tinnitus in a subject including administering to the subject a composition including a bone marrow cellular fraction. In another embodiment, the present invention relates to a method of treating tinnitus in a subject including administering to the subject a composition including adult stem cells.

Abstract: The present invention provides improved methods for osteoarthritis therapy. In particular, the present invention provides a method for predicting the responsiveness of a subject to cell therapy for osteoarthritis comprising administering to the subject platelet-rich plasma (PRP) and assessing pain and/or mobility, wherein a decrease in pain and/or an increase in mobility indicates that the subject will be responsive to cell therapy.

Abstract: In one embodiment, the present invention relates to a method for reducing the frequency and/or severity of headache in a subject comprising administering to the subject a composition comprising the stromal vascular fraction or stromal cells of adipose tissue. In another embodiment, the present invention relates to a method for reducing the frequency and/or severity of headache in a subject comprising administering to the subject a composition compositing bone marrow. In another embodiment, the present invention relates to a method for reducing the frequency or severity of headache in a subject comprising administering to the subject a composition comprising adult stem cells.

Abstract: In one embodiment, the present invention relates to a non-enzymatic method for isolating stem cells from adipose tissue, wherein the method comprises treating adipose tissue with ultrasonic cavitation to break up the adipose tissue and lyses mature adipocytes, resulting in a stromal vascular fraction containing viable stromal/stem cells.

Abstract: A mounted circuit card is disclosed that has a housing with a circuit card assembly (“CCA”) attached to the housing with adhesive along at least an edge portion of the CCA, where the assembly includes at least one jagged edge portion of the CCA in contact with the adhesive, or at least one channeled portion of the housing in contact with the adhesive, or a combination of both a jagged edge portion of the CCA and channeled portion of the housing in contact with the adhesive.

Abstract: The present invention provides novel methods for producing nucleic acid fragment libraries that express highly diverse peptides or protein domains and, in particular, methods for producing nucleic acid fragment libraries wherein the nucleic acid fragments of the libraries are derived from two or more diverse characterized genomes.

Abstract: A mounted circuit card is disclosed that has a housing with a circuit card assembly (“CCA”) attached to the housing with adhesive along at least an edge portion of the CCA, where the assembly includes at least one jagged edge portion of the CCA in contact with the adhesive, or at least one channeled portion of the housing in contact with the adhesive, or a combination of both a jagged edge portion of the CCA and channeled portion of the housing in contact with the adhesive.

Abstract: The present invention provides antimicrobial peptides and analogs thereof that are cytostatic or cytotoxic toward at least one gram-negative bacterium of the genus Acinetobacter including laboratory or clinical isolates of A. baumannii with low or non-detectable cytotoxicity against mammalian cells. Optionally, the antimicrobial peptides and analogs thereof are also cytotoxic or cytostatic against other unrelated bacteria such as Staphylococcus aureus.

Abstract: The present invention provides novel methods for producing nucleic acid fragment libraries that express highly diverse peptides or protein domains and, in particular, methods for producing nucleic acid fragment libraries wherein the nucleic acid fragments of the libraries are derived from two or more diverse characterized genomes.

Abstract: The present invention relates to the use of immunomodifying agents to effect change in the T helper-type 1 (TH1) or T helper-type 2 (TH2) arms of the immune response and thereby treat TH1 or TH2 mediated diseases. In particular, the present invention relates to a method of altering a specific immune response in an individual comprising: i). administering to an individual in need thereof an effective amount of an antigen in immunotherapeutic form, wherein said immune response is down regulated; and ii). subsequently administering to the individual an effective amount of an immunomodifying agent comprising said antigen in immunogenic form.

Abstract: Methods for producing nucleic acid fragment libraries that express highly diverse peptide domains, wherein the nucleic acid fragments are derived form microorganisms or eukaryotes with compact genomes. Also peptides derived form the libraries wherein the peptides are selected on the basis of their abilities to bind selected target proteins, including c-jun and antibodies raised against dust mite allergens.

Abstract: Isolated DNA encoding allergens of Dermatophagoides (house dust mites) particularly of the species Dermatophagoides farinae and Dermatophagolides pteronyssinus, which are protein allergens or peptides which include at least one epitope of the protein allergen. In particular, DNA encoding two major D. farinae allergens, Der f I and Der f II and DNA encoding a D. pteronyssinus allergen, Der p I. In addition, the proteins or peptides encoded by the isolated DNA, their use as diagnostic and therapeutic reagents and methods of diagnosing and treating sensitivity to house dust mite allergens.

Abstract: The present invention provides a method for identifying a modulator or mediator of a biological activity, which activity includes antigenicity and or immunogenicity, said method comprising the step of: (i) producing a gene fragment expression library derived from defined nucleotide sequence fragments; and (ii) assaying the expression library for at least an amino acid sequence derived from step (i) for a biological activity wherein that activity is different from any activity the amino acid sequence may have in its native environment.

Abstract: Isolated nucleic acids encoding allergens of the species Dermatophagoides pteronyssinus and Dermatophagoides farinae, Der p VII and Der f VII, respectively, are disclosed. A cDNA encoding a peptide having a Der p VII activity and a predicted molecular weight of about 22, 177 daltons is described. A cDNA encoding a peptide having Der f VII activity is also described. The nucleic acids of the invention can be used as probes to detect the presence of Der p VII or Der f VII nucleic acid in a sample or for the recombinant production of peptides having a Der p VII or Der f VII activity. Peptides having a Der p VII or Der f VII activity can be used in compositions suitable for pharmaceutical administration or methods of diagnosing sensitivity to house dust mite allergens.

Abstract: Isolated nucleic acids encoding an allergen of Dermatophagoides pteronyssinus, Der p III, are disclosed. A cDNA encoding a peptide having a Der p III activity and a predicted molecular weight of about 24,985 daltons is also described. The nucleic acids can be used as probes to detect the presence of Der p III nucleic acid in a sample or for the recombinant production of peptides having an activity of Der p III. Peptides having an activity of Der p III can be used in compositions suitable for pharmaceutical administration or methods of diagnosing sensitivity to house dust mites.

Abstract: The present invention provides novel methods for producing nucleic acid fragment libraries that express highly diverse peptides or protein domains and, in particular, methods for producing nucleic acid fragment libraries wherein the nucleic acid fragments of the libraries are derived from two or more diverse characterized genomes.

Abstract: A well production apparatus includes a down-hole gear pump and a transport assembly to which the gear pump is attached. The transport assembly is formed from a string of modular pipe assemblies having one or more passages for carrying production fluid from the bottom of the well to the surface. The passages can be arranged in a side-by-side configuration, and include pressure and return lines such as used to drive a hydraulic motor connected to the gear pump. The modular pipe assemblies can have two, three, four, or more passages, and can be stepped relative to each other to give a unique assembly orientation. The modular assemblies have mating fitting that can be tightened without the need to spin the pipe joints at the same time.