Abstract: The invention relates to a composition and method for prolonging the shelf life of banana by using interfering RNA, said method transfers a control cassette for Musa spp. ACC oxidase into banana by a novel gene transfer method, wherein said composition comprises an interfering RNA, a gene transfer expression vector and pharmaceutically acceptable carrier, wherein said interfering RNA can inhibit/knock-down the mRNA expression of Musa spp. ACC oxidase, inhibit further the biosynthesis of ethylene in banana, thereby delay the ripening of banana, and consequently, prolong the shelf life of banana.

Abstract: The invention provides a promoter that has high expression strength and can be over-expressed in various tissues of plant, said promoter is a promoter for banana polyubiquitin (polyubiquitin) gene MhUBQ1, and has a sequence as SEQ ID No: 3. The invention provides further a gene expression cassette that contains a promoter comprising a DNA sequence as SEQ ID No: 3, and a polynucleotide having a open reading frame (ORF) linked to the 3? terminal of said promoter, wherein said promoter can activate the transcription of said polynucleotide in a organism containing said gene expression cassette. The invention provides further a gene expression vector that contains a promoter having a DNA sequence as SEQ ID No: 3. Also, the invention provides a process for producing a transgenic plant or part of organ, tissue or cell thereof containing the above-described gene expression cassette.

Abstract: A promoter capable of activating expression specifically in both of the actively dividing young tissues and separate-related aging tissues in plants, characterized in that the promoter is a promoter of Oncidium ethylene receptor gene OgERS1, and has a sequence as shown in SEQ ID No: 3. A gene expression cassette is composed of a promoter having a DNA sequence as shown in SEQ ID No: 3, and a polynucleotide that is linked to the 3? terminal of the promoter and has an open reading frame, wherein the promoter can activate the transcription of the polynucleotide in a organism having the gene expression cassette therein. A gene expression vector is composed of a promoter having a DNA sequence as shown in SEQ ID No: 3. A plant or parts of organ, tissue or cell of the plant has been transformed to contain the gene expression cassette described above.

Abstract: The invention provides a promoter that has high expression strength and can be over-expressed in various tissues of plant, said promoter is a promoter for banana polyubiquitin (polyubiquitin) gene MhUBQ1, and has a sequence as SEQ ID No: 3. The invention provides further a gene expression cassette that contains a promoter comprising a DNA sequence as SEQ ID No: 3, and a polynucleotide having a open reading frame (ORF) linked to the 3? terminal of said promoter, wherein said promoter can activate the transcription of said polynucleotide in a organism containing said gene expression cassette. The invention provides further a gene expression vector that contains a promoter having a DNA sequence as SEQ ID No: 3. Also, the invention provides a process for producing a transgenic plant or part of organ, tissue or cell thereof containing the above-described gene expression cassette.

Abstract: The invention relates to a composition and method for prolonging the shelf life of banana by using interfering RNA. Said method transfers a control cassette for Musa spp. ACC oxidase into banana by a novel gene transfer method, wherein said composition comprises an interfering RNA, a gene transfer expression vector and pharmaceutically acceptable carrier. Said interfering RNA can inhibit/knock-down the mRNA expression of Musa spp. ACC oxidase and inhibit the biosynthesis of ethylene in banana, thereby delaying the ripening of banana, and consequently, prolonging the shelf life of banana.

Abstract: The invention provides an anther-specific expression promoter in plant, wherein said promoter is a promoter of Oncidium aureusidin synthase gene OgAS1, and has a sequence as SEQ ID No: 3. The invention provides further a gene expression cassette that comprised a promoter having a DNA sequence as SEQ ID No: 3, and a polynucleotide that encode an open reading frame and is linked to the 3? end of said promoter, wherein said promoter can activate the transcription of said polynucleotide in an organism containing said gene expression cassette. The invention provides also a gene expression vector that contains a promoter having DNA sequence as SEQ ID No: 3. The invention provides further a process for producing a transgenic plant or part of organ, tissue or cell of said transgenic plant containing the above-described gene expression cassette.

Abstract: The invention provides an anther-specific expression promoter in plant, wherein said promoter is a promoter of Oncidium aureusidin synthase gene OgAS1, and has a sequence as SEQ ID No: 3. The invention provides further a gene expression cassette that comprised a promoter having a DNA sequence as SEQ ID No: 3, and a polynucleotide that encode an open reading frame and is linked to the 3? end of said promoter, wherein said promoter can activate the transcription of said polynucleotide in an organism containing said gene expression cassette. The invention provides also a gene expression vector that contains a promoter having DNA sequence as SEQ ID No: 3. The invention provides further a process for producing a transgenic plant or part of organ, tissue or cell of said transgenic plant containing the above-described gene expression cassette.

Abstract: A promoter capable of activating expression specifically in both of the vigorously divisible young tissues and separate-related aging tissues in plants, characterized in that the promoter is a promoter of Oncidium ethylene receptor gene OgERS1, and has a sequence as shown in SEQ ID No: 3. A gene expression cassette is composed of a promoter having a DNA sequence as shown in SEQ ID No: 3, and a polynucleotide that is linked to the 3? terminal of the promoter and has an open reading frame, wherein the promoter can activate the transcription of the polynucleotide in a organism having the gene expression cassette therein. A gene expression vector is composed of a promoter having a DNA sequence as shown in SEQ ID No: 3. A plant or parts of organ, tissue or cell of the plant has been transformed to contain the gene expression cassette described above.