Abstract: The present disclosure provides an engineered Hansenula fungus that efficiently expresses CA10 (Coxsackievirus A10) virus-like particles and uses thereof. The engineered fungus includes a recombinant vector carrying the P1 and 3CD genes of the CA10 virus optimized according to preferred codons of Hansenula. The present disclosure also provides a preparation method for CA10 virus-like particles and vaccines prepared therefrom.

Abstract: The present invention provides a Hansenula engineering fungus that efficiently expresses CA10 virus-like particles and use thereof. The engineering fungus comprises a recombinant vector carrying P1 and 3CD genes of the CA10 virus, and the starting strain of the engineering fungus is uracil auxotroph. The Hansenula AU-0501, P1 and 3CD genes are optimized according to preferred codons of Hansenula. The present invention also provides a preparation method of CA10 virus-like particles, comprising: culturing engineering fungi, expressing CA10 virus-like particles, and separating and purifying virus-like particles by ultrafiltration and three-step chromatography.

Abstract: A container module includes a baseplate assembly and a plurality of columns, where the baseplate assembly includes a base frame and a baseplate fixed on the base frame, the plurality of columns are fixed on the base frame in parallel to each other, each column has an extension section extending above the baseplate assembly, and/or a lower end of each column has an extension section extending below the baseplate assembly. A container assembly includes at least two container modules that are stacked vertically and/or connected laterally. A data center includes a function device and the container module.