Abstract: A rapid PCR detection device includes a body, a disposable microfluidic unit, a magnetron micro-fluid unit, a linear actuator, a PCR thermal cycling unit and an image recognition unit. The microfluidic unit is made of a transparent material, wherein a transparent film is arranged in the middle of the microfluidic channel and has at least one hole for a micro-fluid to flow in the microfluidic channel. The magnetron micro-fluid unit drives the micro-fluid, so that the micro-fluid is divided into a plurality of droplets each guided to a lower layer of the microfluidic channel. The linear actuator drives the disposable microfluidic unit to an amplification zone. The PCR thermal cycling unit performs PCR thermal cycling in the amplification zone. The image recognition unit illuminates the droplets with a fluorescent light and determines the number of DNA fragments in the droplets according to the detected fluorescent intensity.