Abstract: The present disclosure may disclose methods and systems for treating an object. The method may include imaging an object fixed on a positioning device using an imaging device. The method may include obtaining a plan image of the object. The method may include generating information of a region of interest (ROI) of the object based on the plan image of the object. The method may include generating a treatment plan based on the information of the ROI. The treatment plan may include a plan isocenter on the plan image. The method may further include treating a target portion of the object based on the treatment plan using a treatment device. The object may be fixed on the positioning device from a moment that the object is started to fixed on the positioning device to an end of the treatment of the target portion.

Abstract: The different advantageous embodiments provide a method for loading and editing text files. A text file is identified as input. A determination is made as to whether the text file is a columnar text file. If the text file is columnar, data information is retrieved for the text file. Pre-defined cache settings are identified. A determination is made as to whether the data information retrieved fits within the pre-defined cache settings. If the data information retrieved fits within the pre-defined cache settings, a determination is made as to whether the first row of the text file is a header row in a view. If the first row of the text file is not a header row, the text file is normalized. A view mode is selected for load and edit operations of the text file.

Abstract: The different advantageous embodiments provide a method for loading and editing text files. A text file is identified as input. A determination is made as to whether the text file is a columnar text file. If the text file is columnar, data information is retrieved for the text file. Pre-defined cache settings are identified. A determination is made as to whether the data information retrieved fits within the pre-defined cache settings. If the data information retrieved fits within the pre-defined cache settings, a determination is made as to whether the first row of the text file is a header row in a view. If the first row of the text file is not a header row, the text file is normalized. A view mode is selected for load and edit operations of the text file.

Abstract: According to a preferred embodiment, a method for identifying the analytical transitional cross-sections of a rigid transport element is disclosed. The method includes providing a 3D model of a rigid transport element, a list of total edges from the 3D model, a list of total faces from the 3D model, and a list of connecting edges from the 3D model, identifying all edges from said list of total edges which have connectivity with any edge in the list of connecting edges and are part of a cross-section, placing the identified edges into a list of cross-section edges, and identifying the analytical cross-section that lies on the plane defined by the edges in said list of cross-section edges.

Abstract: According to a preferred embodiment, a method for identifying the analytical transitional cross-sections of a rigid transport element is disclosed. The method includes providing a 3D model of a rigid transport element, a list of total edges from the 3D model, a list of total faces from the 3D model, and a list of connecting edges from the 3D model, identifying all edges from said list of total edges which have connectivity with any edge in the list of connecting edges and are part of a cross-section, placing the identified edges into a list of cross-section edges, and identifying the analytical cross-section that lies on the plane defined by the edges in said list of cross-section edges.

Abstract: Construction of a recombinant gene fusion encoding a human IgG1 heavy chain constant region and a single-chain variable fragment antibody of 1A4A1 monoclonal antibody is disclosed. The recombinant antibody of the present invention confers human immune effector functions on murine antibodies. After expression in bacteria as inclusion bodies, the recombinant antibody was purified and refolded in vitro. The recombinant soluble antibody retains high antigen-binding affinity to VEE and possesses some human IgG crystallizable fragment domain functions. On non-reducing gel electrophoresis analysis, disulfide bond formation was found in the hinge region of the recombinant antibody. The present invention shows that the recombinant antibody is in a native, functionally active form and it provides the basis to characterize the recombinant antibody for efficacy in vivo.

Abstract: A recombinant gene encoding a single-chain variable fragment (scFv) antibody against Venezuelan equine encephalitis virus (VEE) being cloned into a prokaryotic T7 RNA polymerase-regulated expression vector was disclosed. A streptavidin-binding peptide (SBP) sequence fused to a 6His tag is then attached downstream to the scFv gene. The recombinant fusion protein is expressed in bacteria and then purified by immobilized metal affinity chromatography. ELISA and Western blotting results revealed that the fusion protein not only retained VEE antigen binding and specificity properties similar to those of its parent native monoclonal antibody, but also possessed streptavidin-binding activity. This discovery obviates the need for chemical biotinylation of antibodies and the risk associated with antibody denaturation and provides a stable and reproducible reagent for rapid and efficient immunoassay of VEE.

Abstract: According to one embodiment of the present invention, an electronic method for generating a trimline for trimming sheet metal to be stamped to obtain a stamped part is disclosed. The method includes providing a CAD part file representing a part that can be stamped from a sheet metal material; receiving a selection of a part surface on the part to be stamped and a line on the surface; receiving an input of a sheet metal thickness for the sheet metal material; and generating a trimline based on the CAD part file, the line on the surface, and the sheet metal thickness.

Abstract: A genetically biotinylated single chain fragment variable (scFv) antibody against Venezuelan equine encephalitis virus (VEE) being applied in a system consisting of an immunofiltration-enzyme assay (IFA) with a light addressable potentiometric sensor (LAPS) for the rapid identification of VEE is disclosed. The IFA entails formation of an immunocomplex sandwich consisting of VEE, biotinylated antibody, fluoresceinated antibody and streptavidin, capturing the sandwich by filtration on biotinylated membrane, and detecting the sandwich by anti-fluorescein urease conjugate. The concentration ratio of biotinylated to fluoresceinated antibodies is investigated and optimized. The IFA/LAPS assay sensitivity was approximately equal to that of a conventional enzyme-linked immunosorbant assay utilizing polystyrene plates and a chromogenic substrate, however, less time and effort were required for performance of the IFA/LAPS assay.

Abstract: According to one embodiment of the present invention, an electronic method for generating a trimline for trimming sheet metal to be stamped to obtain a stamped part is disclosed. The method includes providing a CAD part file representing a part that can be stamped from a sheet metal material; receiving a selection of a part surface on the part to be stamped and a line on the surface; receiving an input of a sheet metal thickness for the sheet metal material; and generating a trimline based on the CAD part file, the line on the surface, and the sheet metal thickness.

Abstract: A recombinant gene encoding a single-chain variable fragment (scFv) antibody against Venezuelan equine encephalitis virus (VEE) being cloned into a prokaryotic T7 RNA polymerase-regulated expression vector was disclosed. A streptavidin-binding peptide (SBP) sequence fused to a 6His tag is then attached downstream to the scFv gene. The recombinant fusion protein is expressed in bacteria and then purified by immobilized metal affinity chromatography. ELISA and Western blotting results revealed that the fusion protein not only retained VEE antigen binding and specificity properties similar to those of its parent native monoclonal antibody, but also possessed streptavidin-binding activity. This discovery obviates the need for chemical biotinylation of antibodies and the risk associated with antibody denaturation and provides a stable and reproducible reagent for rapid and efficient immunoassay of VEE.

Abstract: A genetically biotinylated single chain fragment variable (scFv) antibody against Venezuelan equine encephalitis virus (VEE) being applied in a system consisting of an immunofiltration-enzyme assay (IFA) with a light addressable potentiometric sensor (LAPS) for the rapid identification of VEE is disclosed. The IFA entails formation of an immunocomplex sandwich consisting of VEE, biotinylated antibody, fluoresceinated antibody and streptavidin, capturing the sandwich by filtration on biotinylated membrane, and detecting the sandwich by anti-fluorescein urease conjugate. The concentration ratio of biotinylated to fluoresceinated antibodies is investigated and optimized. The IFA/LAPS assay sensitivity was approximately equal to that of a conventional enzyme-linked immunosorbant assay utilizing polystyrene plates and a chromogenic substrate, however, less time and effort were required for performance of the IFA/LAPS assay.

Abstract: Construction of a recombinant gene fusion encoding a human IgG1 heavy chain constant region and a single-chain variable fragment antibody of 1A4A1 monoclonal antibody is disclosed. The recombinant antibody of the present invention confers human immune effector functions on murine antibodies. After expression in bacteria as inclusion bodies, the recombinant antibody was purified and refolded in vitro. The recombinant soluble antibody retains high antigen-binding affinity to VEE and possesses some human IgG crystallizable fragment domain functions. On non-reducing gel electrophoresis analysis, disulfide bond formation was found in the hinge region of the recombinant antibody. The present invention shows that the recombinant antibody is in a native, functionally active form and it provides the basis to characterize the recombinant antibody for efficacy in vivo.