Abstract: An LED light bulb, comprising: a lamp housing, a bulb base, connected with the lamp housing; a stem with a stand extending to the center of the lamp housing, disposed in the lamp housing; a single flexible LED filament, disposed in the lamp housing, points of the flexible LED filament in an xyz coordinates are defined as X, Y, and Z, an x-y plane of the xyz coordinates is perpendicular to the height direction of the light bulb, a z-axis of xyz coordinates is parallel with the stem, the filament is bent into spiral in an axial direction, the diameter of the outer spiral coil of the filament is large than the diameter of the intermediate spiral coil of the LED filament in the YZ plane.

Abstract: An LED light bulb, comprising of: a lamp housing; a bulb base connected to the lamp housing; a stem connected to the bulb base and located in the lamp housing; a single flexible LED filament, disposed in the lamp housing, the flexible LED filament comprising: a plurality of LED sections; a plurality of conductive sections, located between the adjacent two LED sections, where each of the conductive sections includes a conductor, the conductor is copper foil; at least two conductive electrodes; and a light conversion layer comprising a phosphor layer and a silicon layer superposed on the phosphor layer, disposed on the LED chips and at least two sides of the conductive electrodes, and a portion of the conductive electrodes is exposed by the light conversion layer, the LED chip comprising a upper surface and a lower surface opposite to the upper surface of the LED chip, the phosphor layer directly contacts the upper surface of the LED chip, and a base layer contacts the lower surface of the LED chip; the base la

Abstract: An LED light bulb, comprising: a lamp housing, a bulb base, connected with the lamp housing; a stem with a stand extending to the center of the lamp housing, disposed in the lamp housing; a single flexible LED filament, disposed in the lamp housing, points of the flexible LED filament in an xyz coordinates are defined as X, Y, and Z, an x-y plane of the xyz coordinates is perpendicular to the height direction of the light bulb, a z-axis of xyz coordinates is parallel with the stem, where the projection of the flexible LED filament on the XY plane, YZ plane and XZ plane respectively has a length L1, L2 and L3, the length L1, the length L2, and the length L3 are substantially in a ratio of 1:(0.5 to 1):(0.6 to 0.9).

Abstract: An LED light bulb, consisting of: a lamp housing doped with a golden yellow material or its surface coated with a yellow film; a bulb base connected to the lamp housing; a stem connected to the bulb base and located in the lamp housing, the stem comprises a stand extending to the center of the lamp housing; and a single LED filament, disposed in the lamp housing, the LED filament comprising: a light conversion layer, coated on at least two sides of the LED chip and the conductive electrodes, and a portion of each of the conductive electrodes is not coated with the light conversion layer, the light conversion layer has at least one top layer and one base layer, the top layer and the base layer are disposed on the opposing surface of the LED chip, wherein the top layer of the light conversion layer in the conductive section comprises a wavy concave structure with groove, the two adjacent grooves of the wavy concave structure have different width at the positions aligned in the axial direction of the LED filamen

Abstract: An LED light bulb, comprising of: a lamp housing; a bulb base connected to the lamp housing; a stem connected to the bulb base and located in the lamp housing, the stem comprises a stand extending to the center of the lamp housing; a single LED filament, disposed in the lamp housing, the LED filament comprising: a plurality of LED sections, each of the LED sections includes at least two LED chips that are electrically connected to each other; a plurality of conductive sections, each of the conductive sections is located between the two adjacent LED sections and configured to electrically connect the two adjacent LED sections, each of the conductive sections includes at least one conductor that connects the two adjacent LED sections, each of the conductor has at least one through hole; a light conversion layer comprising a base layer and a top layer, wherein the base layer of the light conversion layer is filled into the through hole of the conductor and then contacting the surface of the top layer.

Abstract: An LED light bulb, consisting of: a lamp housing; a bulb base connected to the lamp housing; a stem connected to the bulb base and located in the lamp housing, the stem comprises a stand extending to the center of the lamp housing; a single LED filament, disposed in the lamp housing, the LED filament comprising: a plurality of LED sections, each of the LED sections includes at least two LED chips that are electrically connected to each other by a wire; a plurality of conductive sections, each of the conductive sections is located between the two adjacent LED sections and configured to electrically connect the two adjacent LED sections, each of the conductive section includes a conductor connecting the LED sections, and length of the wire being less than that of the conductor, the conductor comprises joint region and transition regions, the joint region is located between the transition regions and configured to connect the transition regions, wherein taking the central point of the LED chip as the center, the

Abstract: An LED light bulb, consisting of: a lamp housing; a bulb base connected to the lamp housing; a stem connected to the bulb base and located in the lamp housing, a single LED filament, disposed in the lamp housing, the LED filament comprising: two conductive supports, each of the two conductive supports connected with the stem and the flexible LED filament; a driving circuit, electrically connected with both the two conductive supports and the bulb base; and a plurality of supporting arms, each of the supporting arms comprise a first end and a second end opposite to the first end of the supporting arms, the first end of each of the supporting arms is connected with the stand while the second end of each of the supporting arms is connected with the LED filament; where anodes of LED sections are electrically connected together to serve as a first positive electrode P1 and/or a second positive electrode P2 of the flexible LED filament, cathodes of LED section serve as a first negative electrode N1 and/or a second

Abstract: An LED light bulb, consisting of: a lamp housing; a bulb base connected to the lamp housing; a stem connected to the bulb base and located in the lamp housing, the stem comprises a stand extending to the center of the lamp housing; a single LED filament, disposed in the lamp housing, the LED filament comprising: a plurality of LED sections, each of the LED sections includes at least two LED chips that are electrically connected to each other by a wire; a plurality of conductive sections, each of the conductive sections is located between the two adjacent LED sections and configured to electrically connect the two adjacent LED sections, each of the conductive section includes a conductor connecting the LED sections, and length of the wire being less than that of the conductor, the conductor comprises joint region and transition regions, the joint region is located between the transition regions and configured to connect the transition regions, each of the transition region has a opening, and the LED chip close

Abstract: An LED light bulb, comprising of: a lamp housing; a bulb base connected to the lamp housing; a stem connected to the bulb base and located in the lamp housing, the stem comprises a stand extending to the center of the lamp housing; a single LED filament, disposed in the lamp housing, the LED filament comprising: a light conversion layer, coated on at least two sides of the LED chip and the conductive electrodes, and a portion of each of the conductive electrodes is not coated with the light conversion layer, the light conversion layer has at least one top layer and one base layer, the top layer and the base layer are disposed on the opposing surface of the LED chip, wherein the light emitting surface of the LED chips overlaps with the center of the top layer; and two conductive supports, respectively connected with the stem and the LED filament.

Abstract: An LED filament comprises at least one LED section, a conductive section, two conductive electrodes and a light conversion layer. The conductive section is used to electrically connect two adjacent LED sections. The two conductive electrodes are electrically connected to each of the LED sections. Each of the LED sections includes at least two LED chips electrically connected to each other. The light conversion layer covers the LED sections, the conductive sections and the conductive electrodes, and a part of the two electrodes is exposed respectively. Since the LED filament includes the LED section and the conductive section, when the LED filament is bent, the stress is easily concentrated on the conductive section. Therefore, the breakage probability of the conductive wires connected within the LED section is reduced during bending. The quality of the LED filament and its application is improved.

Abstract: The disclosure provides methods of DNA amplification mediated by DNA ligase. Specifically disclosed is a method of amplifying a target region at DNA level, which comprises repeating cycles of amplification comprising the following steps: denaturing DNA template to obtain single target DNA strands; hybridizing a primer pair comprising an upstream primer and a downstream primer to the target DNA strand, wherein the upstream primer is hybridized to a first nucleic acid sequence of the target region, and the downstream primer is hybridized to a second nucleic acid sequence of the target region; the first nucleic acid sequence is downstream to the second nucleic acid sequence on the target DNA strand, with the downstream primer containing a phosphorylated 5? end; ligating the upstream primer or extension product thereof to the downstream primer or extension product thereof, to obtain a semi-amplification product. This disclosure also discloses a kit used to amplify a target region at DNA level.