Abstract: The invention discloses a medium combination for a rapid propagation medium for blueberry stem segment tissue, wherein the pre-culture medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising 0.1-0.5 mg/L IAA, 0.05-0.6 mg/L CPPU; the rapid culture medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising: 0.2-0.5 mg/L ZT; the seedling growth medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising: 0.1-2.0 mg/L 2-ip; and the rooting medium takes ½ MS medium as basis medium, comprising: 0.3-4.0 mg/L NAA. The invention further discloses a method of using the above medium combination to conduct rapid propagation of blueberry stem segment tissue, the propagation rate of this method is rapid.

Abstract: The invention provides a callus induction medium for blueberry tissue culture, it takes WPM medium as basic medium, comprises: 0.5-5.0 mg/L CPPU and 0.1-0.4 mg/L 2-ip. The present invention also provides a callus culture method of blueberry, the step thereof comprises: inoculating the blueberry explant into the above callus induction medium to conduct induction culture, to form blueberry callus. The present invention also discloses the medium combination and culture method to culture the above blueberry callus to blueberry tissue culture plant. For the above medium and culture method, the differentiation effect is good, efficiency is high, and can conduct continuous differentiation, and the effect to multiple varieties are all better.

Abstract: The invention discloses a medium combination for a rapid propagation medium for blueberry stein segment tissue, wherein the pre-culture medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising 0.1-0.5 mg/L IAA, 0.05-0.6 mg/L CPPU; the rapid culture medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising: 0.2-0.5 mg/L ZT; the seedling growth medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising: 0.1-2.0 mg/L 2-ip; and the rooting medium takes ½ MS medium as basis medium, comprising: 0.3-4.0 mg/L NAA. The invention further discloses a method of using the above medium combination to conduct rapid propagation of blueberry stein segment tissue, the propagation rate of this method is rapid.