Abstract: The present application discloses two anti-GDF15 neutralizing monoclonal antibodies and uses thereof. The variable regions of the light chain and heavy chain of the antibodies G1 and G2 with neutralizing activity are provided. Anti-GDF15 mouse monoclonal antibodies were prepared by the hybridoma technology, anti-GDF15 hybridoma cell lines capable of stable secretion with high specificity were screened out, ascites were prepared to obtain high-specificity and high-affinity anti-GDF15 monoclonal antibodies and two anti-GDF15 monoclonal antibodies with neutralizing activity were identified for the first time by constructing an antibody neutralization detection system.

Abstract: Recombinant human type III collagen for promoting angiogenesis, and a preparation method and an application method thereof are provided, which relate to the field of genetic engineering technologies. The amino acid of the recombinant human type III collagen is as shown in SEQ ID NO: 1. The method replaces nine different sites of a full-length natural type III collagen with a GFOGER sequence from type I collagen specifically binding to integrin sites, thereby enhancing promote angiogenic function without changing a total length of natural full-length amino acids. Meanwhile, the method replaces four natural integrin sites with lower adhesion rates, thus the recombinant type III collagen have higher adhesion, to further promote angiogenesis. Through this modification method, the angiogenesis rate will be increased by more than 37%, and the molecular weight of protein translated by the recombinant human type III collagen is moderate, making it easy to prepare.

Abstract: A ?-glycosidase SS-BGL mutant for modifying ginsenoside and an application thereof are provided, which relate to the field of genetic engineering technologies. The ?-glycosidase SS-BGL mutant is a mutant mutating asparagines at 128th position and 302th position of the amino acid sequence as shown in SEQ ID NO: 1 of ?-glycosidase SS-BGL into aspartic acids respectively. The ?-glucosidase SS-BGL mutant improves the thermal stability of the natural ?-glucosidase SS-BGL at extremely high temperature, and is more conducive to the application of SS-BGL in ginsenoside preparation.

Abstract: A method of in-situ electrolyte preparation in a flow battery includes providing a vanadium-based electrolyte solution having vanadium ions of predominantly vanadium V4+ to a first electrode and a second electrode of at least one cell of a flow battery. The vanadium V4+ at the first electrode is converted to vanadium V3+ and the vanadium V4+ at the second electrode is converted to vanadium V5+ by providing electrical energy to the electrodes. A reducing agent is then provided to the vanadium V5+ at the second electrode to reduce the V5+ to vanadium V4+. The vanadium V3+ at the first electrode is then converted to vanadium V2+ and the vanadium V4+ at the second electrode is then converted to vanadium V5+ by providing electrical energy to the electrodes. A simple method to produce predominantly vanadium V4+ electrolyte from a V5+ source, such as V2O5, is also taught.

Abstract: Disclosed herein is a process for coating a component, comprising applying a bond coat on a substrate of a component; applying a thermal barrier material to the bond coat; and applying a coating containing polynuclear aluminum oxide/hydroxide clusters, to the thermal barrier material. Disclosed herein too is a gas turbine engine component, comprising a superalloy substrate; a bond coat on the substrate; a thermal barrier material on the bond coat; and a coating containing polynuclear aluminum oxide/hydroxide clusters on the thermal barrier material.

Abstract: A ?-glycosidase SS-BGL mutant for modifying ginsenoside and application thereof are provided, which relate to the field of genetic engineering technologies. The ?-glycosidase SS-BGL mutant is ?-glycosidase SS-BGL with the amino acid sequence as shown in SEQ ID NO: 1 having mutation at one or more sites. A single-site mutation includes: A1, making glutamine at 96th position of SEQ ID NO: 1 to be mutated to glutamic acid; A2, making asparagine at 97th position of SEQ ID NO: 1 to be mutated to aspartate; A3, making asparagine at 128th position of SEQ ID NO: 1 to be mutated to aspartate; A4, making asparagine at 302th position of SEQ ID NO: 1 to be mutated to aspartate. A double-site mutation is a combination of A3 and A4, a three-site mutation is a combination of A1, A2 and A4, and a four-site mutation is a combination of A1, A2, A3 and A4.

Abstract: A ?-glycosidase SS-BGL mutant for modifying ginsenoside and application thereof are provided, which relate to the field of genetic engineering technologies. The ?-glycosidase SS-BGL mutant is ?-glycosidase SS-BGL with the amino acid sequence as shown in SEQ ID NO: 1 having mutation at one or more sites. A single-site mutation includes: A1, making glutamine at 96th position of SEQ ID NO: 1 to be mutated to glutamic acid; A2, making asparagine at 97th position of SEQ ID NO: 1 to be mutated to aspartate; A3, making asparagine at 128th position of SEQ ID NO: 1 to be mutated to aspartate; A4, making asparagine at 302th position of SEQ ID NO: 1 to be mutated to aspartate. A double-site mutation is a combination of A3 and A4, a three-site mutation is a combination of A1, A2 and A4, and a four-site mutation is a combination of A1, A2, A3 and A4.

Abstract: A ?-glycosidase SS-BGL mutant for modifying ginsenoside and an application thereof are provided, which relate to the field of genetic engineering technologies. The ?-glycosidase SS-BGL mutant is a mutant mutating asparagines at 128th position and 302th position of the amino acid sequence as shown in SEQ ID NO: 1 of ?-glycosidase SS-BGL into aspartic acids respectively. The ?-glucosidase SS-BGL mutant improves the thermal stability of the natural ?-glucosidase SS-BGL at extremely high temperature, and is more conducive to the application of SS-BGL in ginsenoside preparation.

Abstract: A sealing process includes applying a first reactant to a substrate having a porous structure, the first reactant comprising a chromium (III) precursor and a transition metal precursor and applying a second reactant to the first reactant, the second reactant comprising a rare earth element precursor and an alkaline earth element precursor to form reservoirs of trivalent chromium in pore space of the porous structure, and a physical barrier over the substrate and the reservoirs.

Abstract: A glycosyltransferase BS-YjiC mutant, a construction method and an application thereof provided, relating to the field of genetic engineering technologies. The glycosyltransferase BS-YjiC mutant is obtained by performing site-directed mutation on amino acids at the 125th, 178th, 313th, 125th and 178th, or 125th and 313th positions of a wild-type glycosyltransferase BS-YjiC with the amino acid sequence as shown in SEQ ID NO: 1. Compared with the wild-type glycosyltransferase BS-YjiC, the glycosyltransferase BS-YjiC mutant is more suitable for catalyzing protopanaxadiol (PPD) to generate rare ginsenosides F12 and Rh2, which is more conducive to the flexibility of the production process.

Abstract: A glycosyltransferase BS-YjiC mutant, a construction method and an application thereof provided, relating to the field of genetic engineering technologies. The glycosyltransferase BS-YjiC mutant is obtained by performing site-directed mutation on amino acids at the 125th, 178th, 313th, 125th and 178th, or 125th and 313th positions of a wild-type glycosyltransferase BS-YjiC with the amino acid sequence as shown in SEQ ID NO: 1. Compared with the wild-type glycosyltransferase BS-YjiC, the glycosyltransferase BS-YjiC mutant is more suitable for catalyzing protopanaxadiol (PPD) to generate rare ginsenosides F12 and Rh2, which is more conducive to the flexibility of the production process.

Abstract: A method is disclosed for treating an anodized metal surface. According to the method, polynuclear clusters comprising aluminum oxide hydroxide are applied to the anodized metal surface.

Abstract: The present disclosure provides a prime lens module and an in-vehicle camera system. From an object side to an imaging side thereof, the prime lens module sequentially includes: a first lens with a negative focal power, a second lens with a negative focal power, a third lens with a positive focal power, a fourth lens with a positive focal power, a fifth lens with a positive focal power, a sixth lens with a negative focal power, and a seventh lens with a positive focal power. The first lens is a meniscus spherical lens. The second lens is a biconcave spherical lens. The third lens is a biconvex spherical lens and bonded to the second lens. The fourth lens is a biconvex spherical lens or a biconvex aspheric lens. The fifth lens is a biconvex spherical lens. The sixth lens is a biconcave spherical lens and bonded to the fifth lens.

Abstract: Methods and operations for controlling effective delivery of fire suppressant are disclosed. The fire suppression system comprises a control valve operatively coupled with a cylinder containing a fire suppressant. The system comprises a controller operatively coupled with the control valve. The controller is configured to command the control valve to open, discharging a flow of the fire suppressant at a first flow rate; and adjust the flow of the fire suppressant to a second flow rate during the discharge.

Abstract: A method of applying a trivalent chromium or chromium-free conversion coating to a metallic substrate including mixing a dye compound that interacts with electromagnetic radiation outside the human visual spectrum but not electromagnetic radiation that is within the human visual spectrum to produce an observable emission into the trivalent chromium or chromium-free conversion coating mixture to allow for inspection of the coating after applied with a correlating electromagnetic radiation source.

Abstract: The present application discloses two anti-GDF15 neutralizing monoclonal antibodies and uses thereof. The variable regions of the light chain and heavy chain of the antibodies G1 and G2 with neutralizing activity are provided. Anti-GDF15 mouse monoclonal antibodies were prepared by the hybridoma technology, anti-GDF15 hybridoma cell lines capable of stable secretion with high specificity were screened out, ascites were prepared to obtain high-specificity and high-affinity anti-GDF15 monoclonal antibodies and two anti-GDF15 monoclonal antibodies with neutralizing activity were identified for the first time by constructing an antibody neutralization detection system.

Abstract: Disclosed herein is a process for coating a component, comprising applying a bond coat on a substrate of a component; applying a thermal barrier material to the bond coat; and applying a coating containing polynuclear aluminum oxide/hydroxide clusters, to the thermal barrier material. Disclosed herein too is a gas turbine engine component, comprising a superalloy substrate; a bond coat on the substrate; a thermal barrier material on the bond coat; and a coating containing polynuclear aluminum oxide/hydroxide clusters on the thermal barrier material.

Abstract: A fire extinguisher includes a cylinder for storing a fire extinguishing agent; a valve assembly disposed on the cylinder, the valve assembly comprising a closed position and an open position, the valve assembly configured to deliver the fire extinguishing agent from within the cylinder when in the open position; and an actuation assembly operably connected to the valve assembly, the actuation assembly comprising a button and a control device, the control device configured to actuate the valve assembly from the closed position to the open position when the button is contacted.

Abstract: A method is disclosed for treating an anodized metal surface. According to the method, polynuclear clusters comprising aluminum oxide hydroxide are applied to the anodized metal surface.

Abstract: A method is disclosed for treating an anodized metal surface. According to the method, polynuclear clusters comprising aluminum oxide hydroxide are applied to the anodized metal surface.