Abstract: The invention provides a fusion protein comprising the Plasmodium merozoite surface protein-1 (MSP1) and the Plasmodium apical membrane antigen 1 (AMA-1), the encoding DNA sequence, the vector containing the sequence, the host cell containing the vector, and the genetic engineering method for preparing the fusion protein and the usage for producing anti-malarial vaccine. The AMA-1/MSP1 fusion protein of the present invention has excellent immunogenicity and can cause an effective immune response against Plasmodium in individuals.

Abstract: The present invention relates to a method for producing recombinants intended for use in the complete cell-surface protein gp190/MSP1 from plasmodium, especially plasmodium falciparum, as well as the complete DNA sequence of this protein and the appropriate host organisms suited for expressing said sequence, whereby the protein concerned can be entirely synthesized outside the parasite. Also, the inventive method enables sufficient production of above-mentioned protein and its supply as a vaccine. Finally disclosed is a process for stabilizing genes with high At concentration.

Abstract: Recombinant manufacturing process for a complete malaria antigen gp190/MSP1 The invention concerns a recombinant manufacturing process for the complete gp190/MSP1 surface protein of Plasmodium, in particular P. falciparum, as well as the complete DNA sequence of this protein and suitable host organisms for expression of the sequence, by means of which the protein in its entirety can for the first time be synthesized outside the parasite. The invention opens up for the first time the possibility of manufacturing the gp190/MSP1 surface protein in sufficient quantity; in addition there is the matter of making gp190/MSP1 available as a vaccine. Finally the invention sets out a process for the stabilization of AT-rich genes (FIG. 1).

Abstract: The invention provides a fusion protein comprising the Plasmodium merozoite surface protein-1 (MSP1) and the Plasmodium apical membrane antigen 1 (AMA-1), the encoding DNA sequence, the vector containing the sequence, the host cell containing the vector, and the genetic engineering method for preparing the fusion protein and the usage for producing anti-malarial vaccine. The AMA-1/MSP1 fusion protein of the present invention has excellent immunogenicity and can cause an effective immune response against Plasmodium in individuals.