Abstract: A myogenin-expressing fibroblast-like cell (MEFLC) line and a construction method and a use thereof are provided. The MEFLC line was deposited in the Guangdong Microbial Culture Collection Center (GDMCC) on Apr. 23, 2022 with an accession number of GDMCC NO: 62409. The construction method includes: 1) constructing a pCW-MYOG-T2A-Puro lentivirus; 2) infecting a human induced pluripotent stem cell (iPSC) line with the lentivirus, inducing the expression of an MYOG gene, and screening out a positive monoclonal cell line; 3) subjecting the positive monoclonal cell line to expanded cultivation, during which the expression of the MYOG gene is continuously induced; and 4) changing cultivation conditions, continuously inducing the expression of the MYOG gene, and screening out positive monoclonal cell lines to ensure the purity of MYOG-positive cells until a cell morphology changes significantly into fibroblastoid cells to obtain the MEFLC line.

Abstract: A marker and product for auxiliary diagnosis of valvular heart disease (VHD) is provided. The present disclosure provides for the first time using PLAUR as a biomarker for auxiliary diagnosis of VHD. When the PLAUR is used for auxiliary diagnosis, the diagnostic result can be obtained in only one working day (high detection speed) with sensitivity/accuracy is much higher than the sensitivity/accuracy of NT-ProBNP and hsCRP, and a high-throughput operation is enabled.

Abstract: A process for preparing methylene bis-(dialkylamino-dithioformate) in one step includes: simultaneously feeding all or part of four raw materials: dialkylamine, an aqueous solution of sodium hydroxide, dichloromethane and carbon disulfide through a constant-flow pump into a continuous flow reactor; performing the reactions in the continuous flow reactor under a temperature of 10 to 100° C. and with a residence time of 10 to 100 s; simply separating of the obtained reaction products to give the final product. The process synthesizes the product in one step by using the continuous flow reactor. The rapid mass transfer and heat transfer in the continuous flow reactor promote the main reaction, reduce side reactions, improve the product color, and shorten the operation time. Moreover, the yield is relatively high and the quality of the final product meets the requirements.

Abstract: A process for preparing methylene bis-(dialkylamino-dithioformate) in one step includes: simultaneously feeding all or part of four raw materials: dialkylamine, an aqueous solution of sodium hydroxide, dichloromethane and carbon disulfide through a constant-flow pump into a continuous flow reactor; performing the reactions in the continuous flow reactor under a temperature of 10 to 100° C. and with a residence time of 10 to 100 s; simply separating of the obtained reaction products to give the final product. The process synthesizes the product in one step by using the continuous flow reactor. The rapid mass transfer and heat transfer in the continuous flow reactor promote the main reaction, reduce side reactions, improve the product color, and shorten the operation time. Moreover, the yield is relatively high and the quality of the final product meets the requirements.

Abstract: Technologies are generally described for effectively damping the vibration of a platform. In some examples, a platform system for minimizing shock to an object includes a base, a platform effective to carry the object, a number of actuators coupled between the platform and the base and being configured to adjust an orientation of the platform based on control signals, a number of accelerometers positioned on the base, and a processor coupled to the accelerometers and the actuators. The processor is programmed to collect acceleration data from the accelerometers, determine an acceleration vector of the platform from the acceleration data, compensate for the acceleration vector by determining travels and travel directions for the actuators in an effort to maintain contact between the platform and the object, and generate control signals to actuate the actuators with the travels in the travel directions.

Abstract: Technologies are generally described for effectively damping the vibration of a platform. In some examples, a platform system for minimizing shock to an object includes a base, a platform effective to carry the object, a number of actuators coupled between the platform and the base and being configured to adjust an orientation of the platform based on control signals, a number of accelerometers positioned on the base, and a processor coupled to the accelerometers and the actuators. The processor is programmed to collect acceleration data from the accelerometers, determine an acceleration vector of the platform from the acceleration data, compensate for the acceleration vector by determining travels and travel directions for the actuators in an effort to maintain contact between the platform and the object, and generate control signals to actuate the actuators with the travels in the travel directions.