Abstract: We hereby disclose and claim a mathematically based system for design and implementation synthetic biology genetic circuitry architecture which uses genetic expression circuits which are linked together to achieve a therapeutic or diagnostic effect. The genetic architecture that enables the reversible and tunable desired gene product output is achieved by genetic control elements upstream and downstream of a promoter region. Reversible and tunable control is achieved through the computational design and pairing of transcriptional repression elements with their cognate transcriptional promoters to enable a broad range of therapeutic and diagnostic applications.

Abstract: Provided is a modular construction of synthetic gene circuits in mammalian cells using TALE transcriptional repressors.

Abstract: An expression system, comprising: a first nucleic acid molecule having a cell-specific promoter; a second nucleic acid molecule which encodes a transcriptional activator; a third nucleic acid molecule having a first recognition sequence of the transcriptional activator; a fourth nucleic acid molecule having a first promoter and a first regulatory element; a fifth nucleic acid molecule which encodes a first regulatory protein; a sixth nucleic acid molecule having a second recognition sequence of the transcriptional activator; a seventh nucleic acid molecule having a second promoter and a second regulatory element; an eighth nucleic acid molecule which encodes a second regulatory protein; as well as a ninth nucleic acid molecule which is configured to conditionally inhibit the expression of the first regulatory protein, and a tenth nucleic acid molecule which is configured to conditionally inhibit the expression of the second regulatory protein; the first regulatory element is adapted to inhibit the function of

Abstract: Provided is a modular construction of synthetic gene circuits in mammalian cells using TALE transcriptional repressors.