Abstract: The present disclosure relates in some aspects to methods and compositions for in situ detection of an analyte in a biological sample embedded in a matrix that is attached to metal nanoparticles. In some embodiments, the detection involves generation of a fluorescent signal that is enhanced by the metal nanoparticles. The fluorescent signal can be detected at a 3-dimensional (3D) location in the matrix which corresponds to the 3D location of the analyte in the biological sample.

Abstract: The present disclosure relates in some aspects to methods and compositions for assessing system performance for in situ analyte detection. In some aspects, performance of an individual instrument can be assessed, or the performance of two or more instruments can be assessed and optionally compared. In some aspects, disclosed herein is a method comprising using rolling circle amplification products (RCPs) deposited on a cell-free and tissue-free quality control (QC) slide to assess performance of instrument workflow, where an instrument is used to decode signals associated with the RCPs on the QC slide. Quality metrics associated with the decoding (e.g., a percentage of RCPs successfully decoded to genes) can be used to qualify a system comprising the instrument and reagents for in situ analyte detection in cells or tissue samples, e.g., using in situ probe hybridization or in situ sequencing performed on the instrument.

Abstract: A cardiac information dynamic display system comprises: one or more electrodes configured to record sets of electric potential data representing cardiac activity at a plurality of time intervals; and a cardiac information console, comprising: a signal processor configured to: calculate sets of cardiac activity data at the plurality of time intervals using the recorded sets of electric potential data, wherein the cardiac activity data is associated with surface locations of one or more cardiac chambers; and a user interface module configured to display a series of images, each image comprising: a graphical representation of the cardiac activity. Methods of providing cardiac activity data are also provided.

Abstract: A cardiac information dynamic display system comprises: one or more electrodes configured to record sets of electric potential data representing cardiac activity at a plurality of time intervals; and a cardiac information console, comprising: a signal processor configured to: calculate sets of cardiac activity data at the plurality of time intervals using the recorded sets of electric potential data, wherein the cardiac activity data is associated with surface locations of one or more cardiac chambers; and a user interface module configured to display a series of images, each image comprising: a graphical representation of the cardiac activity. Methods of providing cardiac activity data are also provided.

Abstract: A cardiac information dynamic display system comprises: one or more electrodes configured to record sets of electric potential data representing cardiac activity at a plurality of time intervals; and a cardiac information console, comprising: a signal processor configured to: calculate sets of cardiac activity data at the plurality of time intervals using the recorded sets of electric potential data, wherein the cardiac activity data is associated with surface locations of one or more cardiac chambers; and a user interface module configured to display a series of images, each image comprising: a graphical representation of the cardiac activity. Methods of providing cardiac activity data are also provided.

Abstract: Embodiments of the present invention provide a method and a system for controlling fuel supply of a dual-fuel vehicle. The method includes: detecting an operation status of a pressure sensor by an electronic control unit, when an engine is at a gas state; receiving a pressure signal of the pressure sensor by the electronic control unit, when the pressure sensor works properly, the pressure signal indicating internal pressure of a gas tank; and controlling the engine to switch to fuel state by the electronic control unit, when the duration time of the internal pressure of the gas tank less than or equal to a predetermined pressure is greater than or equal to a predetermined time. The method can accurately control switching states according to the remainder of the gas in the gas tank, therefore avoid the engine frequent gas-fuel.

Abstract: In one aspect there is provided an embodiment of an image capture device comprising a camera, an image processor, a storage device and an interface. The camera is configured to capture images in ambient light of a human hand in a field of view (FOV) of the camera. The image processor is configured to process a first one of the images to detect a presence of the hand. The image capture device is configured to assign a position of the presence of the hand, track movement of the hand within the FOV by processing at least a second one of the images and generate a command based on the tracked movement of the hand within the FOV. The interface is configured to transmit the detection of the presence of the hand, the assigned position of the hand and the command to an external apparatus.

Abstract: One aspect provides an image capture device which, in one embodiment, includes a camera, image processor, and interface. The image processor is configured to determine a location of at least one eye of a presenter in a captured image captured by the camera. The image processor is also configured to cause a projection control processor external to the image capture device to modify a corresponding location of a projectable image.

Abstract: In one aspect there is provided an embodiment of an image capture device comprising a camera, an image processor, a storage device and an interface. The camera is configured to capture images in ambient light of a human hand in a field of view (FOV) of the camera. The image processor is configured to process a first one of the images to detect a presence of the hand. The image capture device is configured to assign a position of the presence of the hand, track movement of the hand within the FOV by processing at least a second one of the images and generate a command based on the tracked movement of the hand within the FOV. The interface is configured to transmit the detection of the presence of the hand, the assigned position of the hand and the command to an external apparatus.

Abstract: In one aspect there is provided an embodiment of an image capture device comprising a camera, an image processor, a storage device and an interface. The camera is configured to capture images in visible spectrum light of a human finger as part of a human hand in a field of view (FOV) of the camera. The image processor is configured to process a first one of the images to detect a presence of the finger. The image capture device is configured to detect the position of the presence of the finger tip, track movement of the finger tip within the FOV by processing at least a second one of the images and generate a command based on the tracked movement of the finger within the FOV. The method does not require any pre-detection training sequence with said finger prior to finger detection, and does not require the finger to be in specific relative angle or finger orientation in said FOV.