Abstract: A snow removal device and snow removal equipment. The snow removal device includes a machine body assembly and a snow-discharging rotary drum, in which the machine body assembly includes a machine body and a transition ring seat; the machine body has a machine body snow outlet; the transition ring seat is connected to the machine body and arranged surrounding the machine body snow outlet; the transition ring seat has an inner ring surface, and an end of the inner ring surface facing away from the machine body snow outlet has a first circular rim; and an inlet end of the snow-discharging rotary drum is rotatably connected to the machine body assembly, and the inlet end has a second circular rim that overlaps the first circular rim.

Abstract: Provided are a modified Klenow fragment and an application thereof; specifically provided is a modified Klenow fragment, wherein at least one position or functionally equivalent position among F762, A842, I709 and P603 in the amino acid sequence of the modified Klenow fragment contains at least one amino acid substitution mutation. The modified Klenow fragment has higher DNA polymerase activity than a wild-type Klenow fragment and can be applied to sequencing.

Abstract: The present application discloses a base calling method and apparatus, an electronic device, and a storage medium. The method for base calling includes: determining a first sequencing information based on intensity features of a first spot in images corresponding to a consecutive cycles of base extension reactions including a designated cycle of base extension reaction, where a is a natural number greater than or equal to 1; and determining a base type of the designated cycle of base extension reaction based on the first sequencing information and a basecall model, where the basecall model is determined based on a second sequencing information corresponding to the a consecutive cycles of base extension reactions in a training sample and base type of at least one cycle of base extension reaction in the a consecutive cycles of base extension reactions, and the second sequencing information includes the first sequencing information.

Abstract: The present application relates to the field of sequencing, and specifically relates to a method for adjusting a first optical parameter during sequencing and an application thereof. The present application discloses an adaptive optical adjustment method, which involves imaging a small region on a chip, estimating the sequencing brightness, and adjusting the laser power or exposure time to ensure that the base brightness falls within the range meeting the base calling requirements. This helps the sequencer stabilize sequencing signals despite performance fluctuations in the chip, a laser, a reagent, or an optical system.

Abstract: Provided are a method and device for determining fetal DNA concentration. The method for determining the concentration of fetal DNA includes: acquiring multiple sequencing reads of a cell-free DNA (cfDNA) sample under test; aligning the multiple sequencing reads with a reference genome to obtain alignment results; and determining the concentration of fetal DNA based on the alignment results. The concentration of fetal DNA can be determined based on the alignment results between the sequencing reads and the reference genome so that applications to various sequencing platforms including a single-molecule sequencing platform can be satisfied, and the accuracy of determination of the concentration of fetal DNA can be improved.

Abstract: Provided are a modified Klenow fragment and an application thereof; specifically provided is a modified Klenow fragment, wherein at least one position or functionally equivalent position among F762, A842, 1709 and P603 in the amino acid sequence of the modified Klenow fragment contains at least one amino acid substitution mutation. The modified Klenow fragment has higher DNA polymerase activity than a wild-type Klenow fragment and can be applied to sequencing.

Abstract: Disclosed are a method and an apparatus for base calling, a device, and a storage medium. The method includes: acquiring a first mapping relationship between correct/incorrect classification information of an initial base calling result of a sequencing cycle and first sequencing information of the initial base calling result of a sequencing cycle, where the first sequencing information includes first initial base calling information based on a designated sequencing cycle; and determining the correct/incorrect classification information of the initial base calling result of a sequencing cycle to be processed based on the first mapping relationship and the first sequencing information of the sequencing cycle to be processed. The method for base calling according to the method reduces the impact of a contextual sequence on the base calling and improves the accuracy of the base calling.

Abstract: Disclosed are a fluorescence detection reagent, a method for incorporating a labeled nucleotide by using the reagent, and a kit or a mixture system. The fluorescence detection reagent comprises an enzymatic deoxygenation system and a plurality of reducing agents, and does not comprise DABCO. The composition is suitable for an application scenario in relation to fluorescence signal detection, in particular an application comprising single molecule fluorescence signal detection.

Abstract: The present application discloses a method and an apparatus for determining a quality parameter in basecall. The method comprises: acquiring calling result data of a basecall model for a nucleic acid sample of interest, the calling result data comprising base probability distribution for a current base extension reaction in the nucleic acid sample of interest; calculating a first parameter on the basis of the base probability distribution; and filtering the first parameters on the basis of a preset Q0 value to give a target quality parameter. In the present application, the target quality parameter is determined using the information output by the basecall model, such that the target quality parameter can more accurately determine the basecall results learned by a machine.

Abstract: Provided are a modified Klenow fragment and an application thereof; specifically provided is a modified Klenow fragment, wherein at least one position or functionally equivalent position among F762, A842, I709 and P603 in the amino acid sequence of the modified Klenow fragment contains at least one amino acid substitution mutation. The modified Klenow fragment has higher DNA polymerase activity than a wild-type Klenow fragment and can be applied to sequencing.