Abstract: The present application provides a detection system and a detection method of genomic carcinogenesis information based on cell-free DNA, particularly plasma cell-free DNA. The system includes a library construction apparatus, a sequencing apparatus and an information analysis apparatus, the library construction apparatus is configured to convert 5-methylcytosine (5-mC) in the cell-free DNA in a to-be-detected sample into 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC) and convert non-methylated cytosine (C) into uracil (U) by using enzymes, and the information analysis apparatus is capable of analyzing methylation density of genome, fragment size distribution, fragment 5? end motif and/or chromosome stability. With the adoption of the system and the method, early, sensitive and accurate detection and screening of various cancers can be synchronously implemented.

Abstract: The invention relates to a method for discovering specific functional antibodies, in particular to a method for discovering specific functional antibodies based on sequence composition and frequency analysis of variable regions of immune host antibodies. Compared with conventional high-throughput antibody sequencing method, this method can quickly and accurately obtain the full-length sequence of the variable region of antibody containing candidate CDR3, the success rate of antibody gene pairing is high and it is suitable for the detection of few samples, and improves the efficiency of obtaining specific functional antibodies.

Abstract: A method and a kit for determining genome instability based on next generation sequencing (NGS) are disclosed. The new method is used to determine whether there is homologous recombination defect by calculating a comprehensive value of one or more of pathogenic germline and somatic mutations, such as SNV, indels, and CNVs, and Biallelic germline and somatic mutations, pathogenic mutational signature, copy number variation (CNV) in homologous recombination repair (HRR) gene, genomic structural variation and genome instability. The genomics DNA is interrupted and added with an A adapter; then corresponding polymerase chain reaction (PCR) is conducted, and Whole genome sequencing is performed; the hybrid capture is conducted with designed probes of HRR genes and SNPs, and a captured DNA library is subjected to amplification and library sequencing; and then professional bioinformatics software is used for evaluation to determine the homologous recombination deficiency (HRD) status.

Abstract: Methods are disclosed for determining the minimal residual cancer status of an individual utilizing assays that detect cancer associated genetic variation in extracellular DNA. The disclosed methods provide for personalized cancer detection based on the genetic profile of solid cancer tissue of an individual under study. The disclosed methods further provide for noise reduction in the sequencing of extracellular DNA and reduced false positive rates in minimal residual cancer status determination.

Abstract: Methods are disclosed for determining the minimal residual cancer status of an individual utilizing assays that detect cancer associated genetic variation in extracellular DNA. The disclosed methods provide for personalized cancer detection based on the genetic profile of solid cancer tissue of an individual under study. The disclosed methods further provide for noise reduction in the sequencing of extracellular DNA and reduced false positive rates in minimal residual cancer status determination.

Abstract: Embodiments of the invention provides methods for determining a methylation score of DNA and determining a ctDNA Fraction (CTDF) value. Additional embodiments are as described herein.

Abstract: Disclosed are a next generation sequencing-based detection panel for glioma, a detection kit, a detection method and an application thereof. The detection panel contains glioma-related genes and loci, wherein the glioma-related genes and loci contain SNP locus on chromosome 1, SNP locus on chromosome 19, MGMT, ATRX, H3F3A, ACVR1, CTC, HIST1H3B, MLH1, PLCG1, SMO, AKT1, CTNNB1, HIST1H3C, MSH2, PMS2, TERT, ATRX, DAXX, HRAS, MSH6, PPMID, TP53, BCOR, DDX3X, IDH1, MYC, PTCH1 and the like.

Abstract: A device and a method for detecting tumor mutation burden (TMB) based on capture sequencing are disclosed. The device includes: a panel design module configured to uniformly add population single-nucleotide polymorphism (SNP) sites to a genome and screen out gene regions that show the highest consistency with whole exome sequencing (WES); a data acquisition module configured to acquire tissue and plasma samples of a target object and acquire sequencing data of the samples; an alignment module configured to align the sequencing data with a reference genome to acquire mutation data results; a somatic mutation analysis module configured to perform somatic analysis on the mutation data results to obtain somatic mutation results; a filtering module configured to remove unreal mutation sites from the somatic mutation results; and a calculation module configured to calculate the TMB.

Abstract: A method and a kit for determining genome instability based on next generation sequencing (NGS) are disclosed. The new method is used to determine whether there is homologous recombination defect by calculating a comprehensive value of one or more of pathogenic germline and somatic mutations, such as SNV, indels, and CNVs, and Biallelic germline and somatic mutations, pathogenic mutational signature, copy number variation (CNV) in homologous recombination repair (HRR) gene, genomic structural variation and genome instability. The genomics DNA is interrupted and added with an A adapter; then corresponding polymerase chain reaction (PCR) is conducted, and Whole genome sequencing is performed; the hybrid capture is conducted with designed probes of HRR genes and SNPs, and a captured DNA library is subjected to amplification and library sequencing; and then professional bioinformatics software is used for evaluation to determine the homologous recombination deficiency (HRD) status.

Abstract: The invention relates to a method for discovering specific functional antibodies, in particular to a method for discovering specific functional antibodies based on sequence composition and frequency analysis of variable regions of immune host antibodies. Compared with conventional high-throughput antibody sequencing method, this method can quickly and accurately obtain the full-length sequence of the variable region of antibody containing candidate CDR3, the success rate of antibody gene pairing is high and it is suitable for the detection of few samples, and improves the efficiency of obtaining specific functional antibodies.

Abstract: In one embodiment of the present system invention, there is provided a dividing module positioned in the holding tank of a toilet to separate the holding tank into a main portion and a reserve portion. The dividing module has a height lower than the full water level in the holding tank. The dividing module includes the ability to selectively connect the main portion and the reserve portion in fluid communication of each other. The embodiment allows an operator to press the handle arm an initial time to maintain the main portion and the reserve portion in substantially fluid separation of each other, such that water in the main portion is utilized to flush the toilet and wherein when the operator presses the handle a second time in the same manner as the initial time, the dividing module connects the main portion and the reserve portion in substantially fluid communication with each other, such that water in the reserve portion and the main portion is utilized to flush the toilet.

Abstract: In one embodiment of the present system invention, there is provided a dividing module positioned in the holding tank of a toilet to separate the holding tank into a main portion and a reserve portion. The dividing module has a height lower than the full water level in the holding tank. The dividing module includes the ability to selectively connect the main portion and the reserve portion in fluid communication of each other. The embodiment allows an operator to press the handle arm an initial time to maintain the main portion and the reserve portion in substantially fluid separation of each other, such that water in the main portion is utilized to flush the toilet and wherein when the operator presses the handle a second time in the same manner as the initial time, the dividing module connects the main portion and the reserve portion in substantially fluid communication with each other, such that water in the reserve portion and the main portion is utilized to flush the toilet.

Abstract: An edible multilayer moisture barrier for food products is provided for separating food components having different water activities and preventing or significantly inhibiting movement of water between the food components. The edible multilayer moisture barrier of the present invention includes a lipid layer and a flexible hydrophobic layer.

Abstract: An edible multilayer moisture barrier for food products is provided for separating food components having different water activities and preventing or significantly inhibiting movement of water between the food components. The edible multilayer moisture barrier of the present invention includes a lipid layer and a flexible hydrophobic layer.

Abstract: Microwaveable pizza crusts, which when heated in a microwave oven, rise in a manner similar to conventionally-baked pizza crusts. Such pizza crusts are mesophase gel-containing. Moreover, such mesophase-containing pizza crusts, when microwaved, do not only rise as is observed with conventional pizza crusts, but microwave treatment of these pizza crusts results in highly palatable and crisp pizza crusts, similar to those that are conventionally prepared. The mesophase gels formed herein for use in pizza crust are highly viscous, even in the absence of polymeric protein or polysaccharide thickening or bulking agents. The mesophase gel is formed using at least two emulsifiers and an aqueous phase. The addition of the mesophase gel to the other pizza crust components results in a leavening action, and contributes to the palatability and firmness of the resulting pizza crust.

Abstract: Microwaveable sponge cakes, which when heated in a microwave oven, rise in a manner similar to conventionally-baked sponge cakes. Such sponge cakes are mesophase gel-containing. Moreover, such mesophase-containing sponge cakes, when microwaved, do not only rise as is observed with conventional sponge cakes, but microwave treatment of these sponge cakes results in highly palatable and light sponge cakes, similar to those that are conventionally prepared. The mesophase gels formed herein for use in sponge cake are highly viscous, even in the absence of polymeric protein or polysaccharide thickening or bulking agents. The mesophase gels is formed using two emulsifiers and an aqueous phase. The addition of the mesophase gel to the other sponge cake components results in a leavening action, and contributes to the palatability and lightness of the resulting sponge cake.

Abstract: Microwaveable sponge cakes, which when heated in a microwave oven, rise in a manner similar to conventionally-baked sponge cakes. Such sponge cakes are mesophase gel-containing. Moreover, such mesophase-containing sponge cakes, when microwaved, do not only rise as is observed with conventional sponge cakes, but microwave treatment of these sponge cakes results in highly palatable and light sponge cakes, similar to those that are conventionally prepared. The mesophase gels formed herein for use in sponge cake are highly viscous, even in the absence of polymeric protein or polysaccharide thickening or bulking agents. The mesophase gels is formed using two emulsifiers and an aqueous phase. The addition of the mesophase gel to the other sponge cake components results in a leavening action, and contributes to the palatability and lightness of the resulting sponge cake.

Abstract: The present invention relates to microwaveable pizza crusts, which when heated in a microwave oven, rise in a manner similar to conventionally-baked pizza crusts. Such pizza crusts are mesophase gel-containing. Moreover, such mesophase-containing pizza crusts, when microwaved, do not only rise as is observed with conventional pizza crusts, but microwave treatment of these pizza crusts results in highly palatable and crisp pizza crusts, similar to those that are conventionally prepared. The mesophase gels formed herein for use in pizza crust are highly viscous, even in the absence of polymeric protein or polysaccharide thickening or bulking agents. The mesophase gel is formed using at least two emulsifiers and an aqueous phase. The addition of the mesophase gel to the other pizza crust components results in a leavening action, and contributes to the palatability and firmness of the resulting pizza crust.

Abstract: The present invention relates to microwaveable sponge cakes, which when heated in a microwave oven, rise in a manner similar to conventionally baked sponge cakes. Such sponge cakes are mesophase gel-containing. Moreover, such mesophase-containing sponge cakes, when microwaved, do not only rise as is observed with conventional sponge cakes, but microwave treatment of these sponge cakes results in highly palatable and light sponge cakes, similar to those that are conventionally prepared. The mesophase gels formed herein for use in sponge cake are highly viscous, even in the absence of polymeric protein or polysaccharide thickening or bulking agents. The mesophase gels is formed using two emulsifiers and an aqueous phase. The addition of the mesophase gel to the other sponge cake components results in a leavening action, and contributes to the palatability and lightness of the resulting sponge cake.

Abstract: The present invention involves the preparation of a starch-based food product coating containing a mixture of seasoning and liquid and dry ingredients that is applied on the product and subsequently dried to provide a smooth flavor coating on the exterior of the product. The coating comprises a mixture of water, at least one edible oil, at least one native starch, at least one seasoning and, optionally, edible protein and an emulsion stabilizer. The present invention further involves a process for preparing such a coating, comprising disbursing the starch in warm water, adding the edible oil to the starch and water mixture, preparing an oil-in-water emulsion from the mixture, adding at least one seasoning to the emulsion, and mixing the resulting composition at a low speed that ensures uniform distribution of solid particles within the composition while minimizing incorporation of air bubbles.