Abstract: A micro-fluidic detector applied for detecting analytes in a fluid sample is disclosed. The micro-fluidic detector comprises a mixing area, a flow area and at least one detection module. The mixing area has a conductive top plate and a plurality of first electrodes for mixing a first fluid and a second fluid so as to form the fluid sample. The flow area has two second electrodes, positioned side by side, for driving the fluid sample to flow. The detection module is used for detecting analytes in the fluid sample flowing in the flow area. The mixing area uniformly mixes the first fluid and the second fluid via an electric field generated by applying a voltage potential, with respect to the conductive top plate, to one by one of the first electrodes in the mixing area. The flow area drives the fluid sample via an electric field generated by the second electrodes.