Abstract: The present invention relates to a device and an apparatus device for processing a biological and/or chemical sample. The device comprises at least one sample processing chamber having an inlet at a first end and a penetrable sealing layer at a second end that forms at least a part of an inner wall of the sample processing chamber. The sealing layer is adapted to seal off the sample processing chamber from the environment until said sealing layer is penetrated to form an outlet. The device and apparatus also includes an absorption layer, wherein upon penetration of said sealing layer, said absorption layer is in fluid contact with said sealing layer and is capable of absorbing fluid released from said sample processing chamber, via the outlet. The present invention also relates to a fluid separation device. The fluid separation device comprises a penetrable sealing layer adapted to form a wall of a sample processing chamber of the device of the invention.